scholarly journals Directed evolution of GFP with non-natural amino acids identifies residues for augmenting and photoswitching fluorescence

2015 ◽  
Vol 6 (2) ◽  
pp. 1159-1166 ◽  
Author(s):  
Samuel C. Reddington ◽  
Amy J. Baldwin ◽  
Rebecca Thompson ◽  
Andrea Brancale ◽  
Eric M. Tippmann ◽  
...  
Keyword(s):  
Amino Acids ◽  
Genetic Code ◽  
Directed Evolution ◽  
Target Protein ◽  
Natural Amino Acids

Genetic code reprogramming allows proteins to sample new chemistry through targeted introduction of non-natural amino acids. By combining with random codon replacement, residues traditionally overlooked can be identified as instilling new properties on a target protein.

Rational design of a function-based selection method for genetically encoding acylated lysine derivatives

2019 ◽  
Vol 17 (25) ◽  
pp. 6127-6130
Author(s):  
Hui Miao ◽  
Chenguang Yu ◽  
Anzhi Yao ◽  
Weimin Xuan
Keyword(s):  
Amino Acids ◽  
Genetic Code ◽  
Directed Evolution ◽  
Rational Design ◽  
Selection Method ◽  
Genetic Code Expansion

Genetic code expansion depends on the directed evolution of aaRS to recognize non-canonical amino acids. Herein, we reported a function-based method that enables rapidly evolving aaRS for acylated lysine derivatives.

scholarly journals Protein evolution with an expanded genetic code

2008 ◽  
Vol 105 (46) ◽  
pp. 17688-17693 ◽  
Author(s):  
Chang C. Liu ◽  
Antha V. Mack ◽  
Meng-Lin Tsao ◽  
Jeremy H. Mills ◽  
Hyun Soo Lee ◽  
...  
Keyword(s):  
Amino Acids ◽  
Genetic Code ◽  
Directed Evolution ◽  
Unnatural Amino Acids ◽  
Selective Advantage ◽  
Display System ◽  
Antibody Library ◽  
Evolution Of Proteins ◽  
Expanded Genetic Code ◽  
Selection Of

We have devised a phage display system in which an expanded genetic code is available for directed evolution. This system allows selection to yield proteins containing unnatural amino acids should such sequences functionally outperform ones containing only the 20 canonical amino acids. We have optimized this system for use with several unnatural amino acids and provide a demonstration of its utility through the selection of anti-gp120 antibodies. One such phage-displayed antibody, selected from a naïve germline scFv antibody library in which six residues in VH CDR3 were randomized, contains sulfotyrosine and binds gp120 more effectively than a similarly displayed known sulfated antibody isolated from human serum. These experiments suggest that an expanded “synthetic” genetic code can confer a selective advantage in the directed evolution of proteins with specific properties.

Site-specific incorporation of non-natural amino acids into proteins in mammalian cells with an expanded genetic code

2006 ◽  
Vol 1 (6) ◽  
pp. 2957-2962 ◽  
Author(s):  
Nobumasa Hino ◽  
Akiko Hayashi ◽  
Kensaku Sakamoto ◽  
Shigeyuki Yokoyama
Keyword(s):  
Amino Acids ◽  
Genetic Code ◽  
Mammalian Cells ◽  
Site Specific ◽  
Natural Amino Acids ◽  
Expanded Genetic Code

scholarly journals Genetic-code evolution for protein synthesis with non-natural amino acids

2011 ◽  
Vol 411 (4) ◽  
pp. 757-761 ◽  
Author(s):  
Takahito Mukai ◽  
Tatsuo Yanagisawa ◽  
Kazumasa Ohtake ◽  
Masatoshi Wakamori ◽  
Jiro Adachi ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Genetic Code ◽  
Code Evolution ◽  
Natural Amino Acids

Synthesis and characteristics of ethylenediamine-N,N'-di-S-α-isovalerate and isomers of its cobalt(III) complexes

1982 ◽  
Vol 47 (1) ◽  
pp. 210-216 ◽  
Author(s):  
Milan Strašák ◽  
František Bachratý ◽  
Jaroslav Majer
Keyword(s):  
Amino Acids ◽  
Absolute Configuration ◽  
Electronic Absorption ◽  
Electronic Absorption Spectra ◽  
Chemical Parameters ◽  
Methyl Group ◽  
Nmr Data ◽  
Physico Chemical ◽  
Natural Amino Acids ◽  
C Nmr

The synthesis and physico-chemical parameters are described of a new complexone based on natural amino acids, viz. ethylenediamine-N,N'-di-S-α-isovalerate (SS-EDDIV). 1H- and 13C-NMR data revealed that the methyl group in the substance are not equivalent. The isomers of the cobalt(III) complex with the asymmetric tetradentate SS-EDDIV ligand were prepared and separated; their characteristics are given. The absolute configuration of two of the five theoretically feasible isomers was determined based on their electronic absorption spectra and circular dichroism data.

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