Sensitive mutant DNA biomarker detection based on magnetic nanoparticles and nicking endonuclease assisted fluorescence signal amplification

RSC Advances ◽  
2015 ◽  
Vol 5 (26) ◽  
pp. 20020-20024 ◽  
Author(s):  
Na Li ◽  
Zhong Feng Gao ◽  
Bei Hua Kang ◽  
Nian Bing Li ◽  
Hong Qun Luo
Keyword(s):  
Magnetic Nanoparticles ◽  
Signal Amplification ◽  
Fluorescence Signal ◽  
Biomarker Detection ◽  
Background Signal ◽  
Sensitive Mutant ◽  
Nicking Endonuclease ◽  
Low Background ◽  
Target Dna ◽  
Fluorescence Signal Amplification

Amplified fluorescence target DNA detection was developed combining nicking endonuclease assisted target recycling and magnetic nanoparticles with low background signal.

Fluorescence Signal Amplification by Cation Exchange in Ionic Nanocrystals

2009 ◽  
Vol 121 (9) ◽  
pp. 1616-1619 ◽  
Author(s):  
Jishan Li ◽  
Tierui Zhang ◽  
Jianping Ge ◽  
Yadong Yin ◽  
Wenwan Zhong
Keyword(s):  
Cation Exchange ◽  
Signal Amplification ◽  
Fluorescence Signal ◽  
Fluorescence Signal Amplification

scholarly journals A new two-mode fluorescence signal amplification strategy for protease activity assay based on graphene oxide

RSC Advances ◽  
2017 ◽  
Vol 7 (76) ◽  
pp. 47983-47989 ◽  
Author(s):  
Fangfang Wang ◽  
Jie Gao ◽  
Jianwei Zhao ◽  
Wenyue Zhang ◽  
Jie Bai ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Protease Activity ◽  
Fluorescence Signal ◽  
Activity Assay ◽  
Fluorescence Signal Amplification ◽  
Amplification Strategy

A new graphene oxide-based two-mode fluorescence signal amplification strategy for the detection of protease activity has been established.

A supersandwich fluorescence in situ hybridization strategy for highly sensitive and selective mRNA imaging in tumor cells

2016 ◽  
Vol 52 (2) ◽  
pp. 370-373 ◽  
Author(s):  
Jin Huang ◽  
He Wang ◽  
Xiaohai Yang ◽  
Yanjing Yang ◽  
Ke Quan ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tumor Cells ◽  
Signal Amplification ◽  
Fluorescence Signal ◽  
Target Mrna ◽  
Highly Sensitive ◽  
Fluorescence Signal Amplification

This strategy uses two fluorophore-labeled signal probes to generate a supersandwich product, which in turn generates numerous signal probes located at the target mRNA position, resulting in thein situfluorescence signal amplification.

scholarly journals Practical method for superresolution imaging of primary cilia and centrioles by expansion microscopy using an amplibody for fluorescence signal amplification

2020 ◽  
Vol 31 (20) ◽  
pp. 2195-2206 ◽  
Author(s):  
Yohei Katoh ◽  
Shuhei Chiba ◽  
Kazuhisa Nakayama
Keyword(s):  
Signal Amplification ◽  
Unit Volume ◽  
Primary Cilia ◽  
Practical Method ◽  
Fluorescence Signal ◽  
Practical Observation ◽  
High Brightness ◽  
Superresolution Imaging ◽  
Fluorescence Signal Amplification ◽  
Physical Expansion

Expansion microscopy was applied to the observation of primary cilia and centrioles. To compensate for the decrease in fluorescence signal per unit volume inherent in physical expansion, a fluorescence signal amplification tool, called the amplibody, was developed. The method enables the practical observation of cilia and centrioles with high brightness and resolution.

Proximity-dependent protein detection based on enzyme-assisted fluorescence signal amplification

2014 ◽  
Vol 51 ◽  
pp. 255-260 ◽  
Author(s):  
Yuyu Tan ◽  
Qiuping Guo ◽  
Xiayu Zhao ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Protein Detection ◽  
Fluorescence Signal ◽  
Fluorescence Signal Amplification ◽  
Dependent Protein

Fluorescence signal amplification on the gel biochips with a mirror surface and optimization of immunoassay procedure

2009 ◽  
Vol 427 (1) ◽  
pp. 171-174 ◽  
Author(s):  
Zh. I. Zubtsova ◽  
M. A. Filippova ◽  
E. N. Savvateeva ◽  
D. A. Zubtsov ◽  
V. R. Chechetkin ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Fluorescence Signal ◽  
Mirror Surface ◽  
Fluorescence Signal Amplification
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