Highly precise plasmonic and colorimetric sensor based on enzymatic etching of nanospheres for the detection of blood and urinary glucose

RSC Advances ◽  
2015 ◽  
Vol 5 (19) ◽  
pp. 14330-14332 ◽  
Author(s):  
Hongje Jang ◽  
Dal-Hee Min
Keyword(s):  
Glucose Oxidase ◽  
Human Blood ◽  
Ag Nanoparticles ◽  
Glucose Sensor ◽  
Peak Shift ◽  
Glucose Detection ◽  
Plasmon Peak ◽  
Urinary Glucose ◽  
Blood And Urine Samples ◽  
Human Blood And Urine

A highly precise glucose sensor was developed based on plasmon peak shift induced by the glucose oxidase mediated etching of Au–Ag nanoparticles. The platform enabled quantitative glucose detection in human blood and urine samples.

scholarly journals On-Chip Glucose Detection Based on Glucose Oxidase Encapsulated on a Platinum Modified Gold Microband Electrode

2021 ◽  
Author(s):  
Julia Madden ◽  
Colm Barrett ◽  
Fathima Laffir ◽  
Michael Thompson ◽  
Paul Galvin ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Glucose Sensor ◽  
Electrochemical Impedance ◽  
Reference Electrode ◽  
Linear Sweep Voltammetry ◽  
Fast Response ◽  
Small Sample ◽  
Ease Of Use ◽  
Glucose Detection ◽  
Serum Samples

We report a two-step electrodeposition process incorporating glucose oxidase onto a platinum- modified gold microband electrode with an o-phenylenediamine and ß-cyclodextrin mixture. The bare microband electrodes were fabricated on silicon using standard microfabrication methods i.e. lithography and etching techniques. The two-step electrode modification process was characterized using cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The enzymatic based microband biosensor exhibited a linear response to glucose from 2.5-15 mM using both linear sweep voltammetry and chronoamperometric measurements in buffer based solutions. The resulting miniaturized glucose sensor presented a number of advantages such as ease of use, fast response time, measuring within physiologically relevant glucose concentrations in addition to sensing in small sample volumes without the need for an external counter and reference electrode. The biosensor performance was tested in 30 µl volumes of undiluted fetal bovine serum. Whilst a reduction in signal was evident within 100 % serum samples, the sensor achieved linear glucose detection with increasing glucose concentrations (2-12 mM).

scholarly journals On-Chip Glucose Detection Based on Glucose Oxidase Encapsulated on a Platinum Modified Gold Microband Electrode

2021 ◽  
Author(s):  
Julia Madden ◽  
Colm Barrett ◽  
Fathima Laffir ◽  
Michael Thompson ◽  
Paul Galvin ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Glucose Sensor ◽  
Electrochemical Impedance ◽  
Reference Electrode ◽  
Linear Sweep Voltammetry ◽  
Fast Response ◽  
Small Sample ◽  
Ease Of Use ◽  
Glucose Detection ◽  
Serum Samples

We report a two-step electrodeposition process incorporating glucose oxidase onto a platinum- modified gold microband electrode with an o-phenylenediamine and ß-cyclodextrin mixture. The bare microband electrodes were fabricated on silicon using standard microfabrication methods i.e. lithography and etching techniques. The two-step electrode modification process was characterized using cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. The enzymatic based microband biosensor exhibited a linear response to glucose from 2.5-15 mM using both linear sweep voltammetry and chronoamperometric measurements in buffer based solutions. The resulting miniaturized glucose sensor presented a number of advantages such as ease of use, fast response time, measuring within physiologically relevant glucose concentrations in addition to sensing in small sample volumes without the need for an external counter and reference electrode. The biosensor performance was tested in 30 µl volumes of undiluted fetal bovine serum. Whilst a reduction in signal was evident within 100 % serum samples, the sensor achieved linear glucose detection with increasing glucose concentrations (2-12 mM).

Simultaneous analysis of mitotane and its main metabolites in human blood and urine samples by SPE-HPLC technique

2012 ◽  
Vol 26 (11) ◽  
pp. 1308-1314 ◽  
Author(s):  
Ana Mornar ◽  
Miranda Sertić ◽  
Nikša Turk ◽  
Biljana Nigović ◽  
Mirko Koršić
Keyword(s):  
Human Blood ◽  
Simultaneous Analysis ◽  
Urine Samples ◽  
Blood And Urine Samples ◽  
Human Blood And Urine ◽  
Hplc Technique

Simultaneous Determination of Epinephrine and Tyrosine Using a Glassy Carbon Electrode Amplified with ZnO-Pt/CNTs Nanocomposite

2019 ◽  
Vol 15 (2) ◽  
pp. 166-171 ◽  
Author(s):  
Ali Samadzadeh ◽  
Iran Sheikhshoaie ◽  
Hassan Karimi-Maleh
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Simultaneous Analysis ◽  
Urine Samples ◽  
Carbon Electrode ◽  
Linear Dynamic ◽  
Biological Compounds ◽  
Blood And Urine Samples ◽  
Human Blood And Urine

Background: Simultaneous analysis of epinephrine and tyrosine as two effective and important biological compounds in human blood and urine samples are very important for the investigation of human health. Objective: In this research, a highly effective voltammetric sensor fabricated for simultaneous analysis of epinephrine and tyrosine. The sensor was fabricated by the modification of glassy carbon electrode with ZnO-Pt/CNTs nanocomposite (ZnO-Pt/CNTs/GCE). The synthesized nanocomposite was characterized by SEM method. The ZnO-Pt/CNTs/GCE showed two separated oxidation signals at potential ~220 mV and 700 mV for epinephrine and tyrosine, respectively. Also, we detected linear dynamic ranges 0.5-250.0 µM and 1.0-220 µM with a limit of detections 0.1 µM and 0.5 µM for the determination of epinephrine and tyrosine, respectively. The ZnO-Pt/CNTs/GCE was used for the determination of epinephrine and tyrosine in blood serum and human urine samples.

Sign in / Sign up

Export Citation Format

Share Document