Effect of apple, baobab, red-chicory, and pear extracts on cellular energy expenditure and morphology of a Caco-2 cells using transepithelial electrical resistance (TEER) and scanning electron microscopy (SEM)

RSC Advances ◽  
2015 ◽  
Vol 5 (29) ◽  
pp. 22490-22498 ◽  
Author(s):  
Enrico Finotti ◽  
Riccardo Gezzi ◽  
Fabio Nobili ◽  
Ivana Garaguso ◽  
Mendel Friedman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Energy Expenditure ◽  
Cell Line ◽  
Electrical Resistance ◽  
Transepithelial Electrical Resistance ◽  
Intestinal Cell ◽  
Cellular Energy ◽  
Intestinal Cell Line ◽  
Scanning Electron

The present study investigated the effects of four food extracts on the Caco-2 intestinal cell line using a new transepithelial electrical resistance method (TEER) concurrent with electron microscopy (SEM).

scholarly journals Smart conductive inks

2008 ◽  
Vol 2 (4) ◽  
pp. 305-308
Author(s):  
Jose Rangel ◽  
◽  
Alicia del-Real ◽  
Victor Castano ◽  
◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electrical Resistance ◽  
Automatic System ◽  
Ethyl Acrylate ◽  
Conductive Ink ◽  
Scanning Calorimetry ◽  
Acrylic Polymers ◽  
Pressure Sensitive ◽  
Scanning Electron

A novel conductive ink, suitable for employment in a pressure-sensitive automatic system, was prepared and characterized via scanning electron microscopy, FTIR and differential scanning calorimetry. The ink was obtained as a composite by mixing a solution of ethyl acrylate-methyl acrylate (50/50 ratio) copolymer and carbon black and graphite into a solvent standard for acrylic polymers. The ink average electrical resistance ranges from 40 ohms/cm to 150 ohms/cm.

scholarly journals Ultrastructural view of astrocyte-astrocyte and astrocyte-synapse contacts within the hippocampus

2020 ◽  
Author(s):  
Conrad M. Kiyoshi ◽  
Sydney Aten ◽  
Emily P. Arzola ◽  
Jeremy A. Patterson ◽  
Anne T. Taylor ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electrical Resistance ◽  
Spatial Location ◽  
Face Scanning ◽  
Block Face ◽  
Synaptic Boutons ◽  
Neuron Interactions ◽  
Branching Architecture ◽  
Scanning Electron

SummaryAstrocytes branch out and make contact at their interfaces. However, the ultrastructural interactions of astrocytes and astrocytes with their surroundings, including the spatial-location selectivity of astrocyte-synapse contacts, remain unknown. Here, the branching architecture of three neighboring astrocytes, their contact interfaces, and their surrounding neurites and synapses have been traced and 3D reconstructed using serial block-face scanning electron microscopy (SBF-SEM). Our reconstructions reveal extensive reflexive, loop-like processes that serve as scaffolds to neurites and give rise to spongiform astrocytic morphology. At the astrocyte-astrocyte interface, a cluster of process-process contacts were identified, which biophysically explains the existence of low inter-astrocytic electrical resistance. Additionally, we found that synapses uniformly made contact with the entire astrocyte, from soma to terminal processes, and can be ensheathed by two neighboring astrocytes. Lastly, in contrast to densely packed vesicles at the synaptic boutons, vesicle-like structures were scant within astrocytes. Together, these ultrastructural details should expand our understanding of functional astrocyte-astrocyte and astrocyte-neuron interactions.

Cryo-scanning electron microscopy applied to preadipocyte cell line 3T3L1

1998 ◽  
Vol 90 (3) ◽  
pp. 287-287
Author(s):  
Sylvie Marull ◽  
Ashley Wilson ◽  
Magali Zbinden ◽  
Frédérique Poirier ◽  
Gaëlle Saintigny ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Line ◽  
Cryo Scanning Electron Microscopy ◽  
Scanning Electron

Preparation and Stabilization of High Conductivity Zn-Coated Tetra-Needle-Like ZnO Whiskers

2011 ◽  
Vol 197-198 ◽  
pp. 362-366
Author(s):  
Zhi Mei Xia ◽  
Cui Feng Wan ◽  
Sheng Ming Jin ◽  
Mo Tang Tang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Zinc Oxide ◽  
Annealing Time ◽  
Electrical Resistance ◽  
Annealing Temperature ◽  
Electrical Resistance Measurement ◽  
X Ray Diffraction ◽  
X Ray ◽  
Scanning Electron

Tetra-needle-like zinc oxide whiskers (T-ZnOW) were coated with Zn and stabilized with Al2O3consequently. Coating conditions for preparation of low resistivity T-ZnOW were investigated. The Zn-coated T-ZnOW was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and electrical resistance measurement. The SEM and XRD results indicated that the tetra-needle-like structure of ZnO whiskers could be well maintained after coating with Zn and stabilizing with Al2O3. Experiment results showed that the resistivity of T-ZnOW was affected significantly by the annealing temperature, annealing time, coating amount of Zn and original content of Al3+. Al3+ions played an important role in stabilizing the resistivity of Zn-coated T-ZnOW. The resistivity of T-ZnOW decreased considerably from 108to 101Ω•cm when the Zn-coated amount and Al3+original content were 10.0 wt.% and 2.0 at.%, respectively.Introduction.

Encapsulation by Electrospraying of Anticancer Compounds from Jackfruit Extract (Artocarpus heterophyllus Lam): Identification, Characterization and Antiproliferative Properties

2020 ◽  
Vol 20 ◽  
Author(s):  
José Oscar Rivera-Aguilar ◽  
Montserrat Calderón-Santoyo ◽  
Elda Margarita González-Cruz ◽  
Jorge Alberto Ramos-Hernández ◽  
Juan Arturo Ragazzo-Sánchez
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Line ◽  
Whey Protein ◽  
Structural Changes ◽  
Biological Activities ◽  
Biological Properties ◽  
Therapeutic Effects ◽  
Scanning Calorimetry ◽  
Scanning Electron

Background: Compounds with biological activities had been reported in the jackfruit. These compounds are susceptible to structural changes such as isomerization and/or loss of bonds due to environmental factors. Then, the encapsulation for protecting is a necessary process. Objective: In this study, encapsulation of high-value biological compounds (HVBC) was performed using high degree of polymerization agave fructans (HDPAF) and whey protein (WP) as encapsulating materials to preserve the biological properties of the HVBC. Methods: The extract was characterized by HPLC-MS in order to show the presence of compounds with preventive or therapeutic effects on chronic degenerative diseases such as cancer. The micrographs by scanning electron microscopy (SEM), thermal analysis (TGA and DSC), photostabilization and antiproliferation of M12.C3.F6 cell line of capsules were evaluated. Results: The micrographs of the nanocapsules obtained by scanning electron microscopy (SEM) showed spherical capsules with sizes between 700 and 800 nm. No cracks, dents or deformations were observed. The thermogravimetric analysis (TGA) evidenced the decomposition of the unencapsulated extract ranging from 154 to 221 °C. On the other hand, the fructan-whey protein mixture demonstrated that nanocapsules have a thermoprotective effect because the decomposition temperature of the encapsulated extract increased 32.1 °C. Differential scanning calorimetry (DSC) exhibited similar values of the glass transition temperature (Tg) between the capsules with and without extract; which indicates that the polymeric material does not interact with the extract compounds. The photoprotection study revealed that nanocapsules materials protect the jackfruit extract compounds from the UV radiation. Finally, the cell viability on the proliferation of M12.C3.F6 cell line was not affected by powder nanocapsules without jackfruit extract, indicating that capsules are not toxic for these cells. However, microcapsules with jackfruit extract (50 µg/ml) were able to inhibit significantly the proliferation cells. Conclusion: The encapsulation process provides thermoprotection and photostability, and the antiproliferative activity of HVBC from jackfruit extract was preserved.

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