A centrifuge-based stepwise chemical loading disc for the production of multiplex anisotropic metallic nanoparticles

RSC Advances ◽  
2015 ◽  
Vol 5 (3) ◽  
pp. 1846-1851 ◽  
Author(s):  
Byung Hyun Park ◽  
Ji Hyun Lee ◽  
Jae Hwan Jung ◽  
Seung Jun Oh ◽  
Doh C. Lee ◽  
...  
Keyword(s):  
High Throughput ◽  
Metallic Nanoparticles ◽  
Au Nps ◽  
High Throughput Manner ◽  
Chemical Loading

We have proposed a novel rotary microdevice in which multiplex anisotropic Au NPs could be synthesized under diverse conditions in a high-throughput manner.

Kinetic Solvent Effects in Organic Reactions

2017 ◽  
Author(s):  
Belinda Slakman ◽  
Richard West
Keyword(s):  
Solvent Effect ◽  
Reaction Kinetics ◽  
Computational Methods ◽  
High Throughput ◽  
Kinetic Modeling ◽  
Solvent Effects ◽  
Reaction Rates ◽  
Prior Work ◽  
Solvent Phase ◽  
High Throughput Manner

<div> <div> <div> <p>This article reviews prior work studying reaction kinetics in solution, with the goal of using this information to improve detailed kinetic modeling in the solvent phase. Both experimental and computational methods for calculating reaction rates in liquids are reviewed. Previous studies, which used such methods to determine solvent effects, are then analyzed based on reaction family. Many of these studies correlate kinetic solvent effect with one or more solvent parameters or properties of reacting species, but it is not always possible, and investigations are usually done on too few reactions and solvents to truly generalize. From these studies, we present suggestions on how best to use data to generalize solvent effects for many different reaction types in a high throughput manner. </p> </div> </div> </div>

scholarly journals Rapid formation of size-controllable multicellular spheroids via 3D acoustic tweezers

Lab on a Chip ◽  
2016 ◽  
Vol 16 (14) ◽  
pp. 2636-2643 ◽  
Author(s):  
Kejie Chen ◽  
Mengxi Wu ◽  
Feng Guo ◽  
Peng Li ◽  
Chung Yu Chan ◽  
...  
Keyword(s):  
High Throughput ◽  
Potential Field ◽  
Multicellular Spheroids ◽  
Rapid Formation ◽  
Acoustic Tweezers ◽  
High Throughput Manner

A 3D acoustic tweezers platform is developed to fabricate size-controllable multicellular spheroids in a rapid and high-throughput manner, utilizing the acoustic Gor'kov potential field and microstreaming.

A hollow fiber system for simple generation of human brain organoids

2017 ◽  
Vol 9 (9) ◽  
pp. 774-781 ◽  
Author(s):  
Yujuan Zhu ◽  
Li Wang ◽  
Fangchao Yin ◽  
Yue Yu ◽  
Yaqing Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Human Brain ◽  
High Throughput ◽  
Hollow Fiber ◽  
Cell Biology ◽  
Hollow Fibers ◽  
Stem Cell Biology ◽  
Fiber System ◽  
Novel Approach ◽  
High Throughput Manner

Here we present a novel approach to engineer hiPSC-derived brain organoids within hollow fibers in a simple and high throughput manner by integrating biomaterials with stem cell biology.

scholarly journals HypercubeME: two hundred million combinatorially complete datasets from a single experiment

2019 ◽  
Author(s):  
Laura Avino Esteban ◽  
Lyubov R. Lonishin ◽  
Daniil Bobrovskiy ◽  
Gregory Leleytner ◽  
Natalya S. Bogatyreva ◽  
...  
Keyword(s):  
Experimental Data ◽  
High Throughput ◽  
Recursive Algorithm ◽  
Random Mutagenesis ◽  
Higher Order ◽  
Single Experiment ◽  
Manual Curation ◽  
Complete Dataset ◽  
Genotype Space ◽  
High Throughput Manner

AbstractMotivationEpistasis, the context-dependence of the contribution of an amino acid substitution to fitness, is common in evolution. To detect epistasis, fitness must be measured for at least four genotypes: the reference genotype, two different single mutants and a double mutant with both of the single mutations. For higher-order epistasis of the order n, fitness has to be measured for all 2n genotypes of an n-dimensional hypercube in genotype space forming a “combinatorially complete dataset”. So far, only a handful of such datasets have been produced by manual curation. Concurrently, random mutagenesis experiments have produced measurements of fitness and other phenotypes in a high-throughput manner, potentially containing a number of combinatorially complete datasets.ResultsWe present an effective recursive algorithm for finding all hypercube structures in random mutagenesis experimental data. To test the algorithm, we applied it to the data from a recent HIS3 protein dataset and found all 199,847,053 unique combinatorially complete genotype combinations of dimensionality ranging from two to twelve. The algorithm may be useful for researchers looking for higher-order epistasis in their high-throughput experimental data.Availabilityhttps://github.com/ivankovlab/HypercubeME.git.

scholarly journals Optimized RNA-targeting CRISPR/Cas13d technology outperforms shRNA in identifying essential circRNAs

2020 ◽  
Author(s):  
Yang Zhang ◽  
Tuan M. Nguyen ◽  
Xiao-Ou Zhang ◽  
Tin Phan ◽  
John G. Clohessy ◽  
...  
Keyword(s):  
High Throughput ◽  
Biological Effect ◽  
High Rate ◽  
Circular Rnas ◽  
Hairpin Rna ◽  
Guide Rnas ◽  
Rna Targeting ◽  
Bona Fide ◽  
Short Hairpin ◽  
High Throughput Manner

AbstractCircular RNAs (circRNAs) are widely expressed, but their functions remain largely unknown. To study circRNAs in a high-throughput manner, short hairpin RNA (shRNA) screens1 have recently been used to deplete circRNAs by targeting their unique back-splicing junction (BSJ) sites. Here, we report frequent discrepancies between shRNA-mediated circRNA knockdown efficiency and the corresponding biological effect, raising pressing concerns about the robustness of shRNA screening for functional circRNAs. To address this issue, we leveraged the CRISPR/Cas13d system2 for circRNAs functional screenings. We optimized a strategy for designing single guide RNAs to deplete circRNAs. We then performed shRNA and CRISPR/Cas13d parallel screenings and demonstrated that shRNA-mediated circRNAs screening yielded a high rate of false positives phenotypes, while optimized CRISPR/Cas13d led to the identification of bona-fide functional circRNAs. Collectively, we developed a specific and reliable approach to functionalize circRNAs in a high-throughput manner.

scholarly journals Enhancing pre‐clinical in vitro cardiotoxicity assays with bioengineering strategies in a high‐throughput manner

2019 ◽  
Vol 33 (S1) ◽  
Author(s):  
Elliot Fisher ◽  
Hamed Ghazizadeh ◽  
Kevin M Gray ◽  
Nicholas A Geisse
Keyword(s):  
High Throughput ◽  
High Throughput Manner

scholarly journals High Throughput pMHC-I Tetramer Library Production Using Chaperone Mediated Peptide Exchange

2019 ◽  
Author(s):  
Sarah A. Overall ◽  
Jugmohit S. Toor ◽  
Stephanie Hao ◽  
Mark Yarmarkovich ◽  
Son Nguyen ◽  
...  
Keyword(s):  
T Cell ◽  
High Throughput ◽  
Molecular Chaperone ◽  
Robust Method ◽  
Single Experiment ◽  
Mhc I ◽  
Combined Analysis ◽  
Transcription Profiles ◽  
Peptide Exchange ◽  
High Throughput Manner

ABSTRACTPeptide exchange technologies are essential for the generation of pMHC-multimer libraries, used to probe highly diverse, polyclonal TCR repertoires. Using the molecular chaperone TAPBPR, we present a robust method for the capture of stable, empty MHC-I molecules which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner. Combined with tetramer barcoding using multi-modal cellular indexing technology (ECCITE-seq), our approach allows a combined analysis of TCR repertoires and other T-cell transcription profiles together with their cognate pMHC-I specificities in a single experiment.

Green Synthesis of Gold Nanoparticles Using Longan Polysaccharide and their Reduction of 4-nitrophenol and Biological Applications

NANO ◽  
2020 ◽  
Vol 15 (01) ◽  
pp. 2050002 ◽  
Author(s):  
Xiaoyu Zhang ◽  
Liyuan Fan ◽  
Yanshuai Cui ◽  
Tianming Cui ◽  
Shengfu Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Green Synthesis ◽  
Metallic Nanoparticles ◽  
High Catalytic Activity ◽  
Synthetic Method ◽  
Biological Applications ◽  
Au Nps ◽  
Stabilizing Agents ◽  
Highly Dispersed ◽  
Negative Zeta Potential

The green synthesis of gold nanoparticles (Au NPs) for catalytic and biological applications has been drawing great attention. To compare with plant extracts, the polysaccharides may be good reducing and stabilizing agents. In this work, we describe the preparation of longan polysaccharide stabilized gold nanoparticles (Aun-LP NPs) by reduction of gold ions using a green synthetic method. The formation of gold nanoparticles (Au NPs) was confirmed by UV-Vis spectra. TEM showed that Au NPs had a small size (7.8–15.6[Formula: see text]nm) and were highly dispersed without any aggregation. XPS confirmed that the surface elemental composition of Aun-LP NPs was C, O, and Au. DLS demonstrated that Aun-LP NPs had good stability and negative zeta potential. In addition, Aun-LP NPs had high catalytic activity for the reduction of 4-nitrophenol. More importantly, Aun-LP NPs had ignorable cytotoxicity towards HeLa cells and showed good antioxidant activity. Taken together, the results indicated that longan polysaccharide can be used as reducing agents and stabilizers for the preparation of metallic nanoparticles, and the product had wide applications.

scholarly journals Rainbowarray Microsphere-Based Gene Detection Assay

2019 ◽  
Vol 25 (3) ◽  
pp. 258-266
Author(s):  
Ying Xu ◽  
Pei Zhang ◽  
Fan Yi ◽  
Jing Wei ◽  
Quan Du
Keyword(s):  
Target Detection ◽  
High Throughput ◽  
Quantitative Measurement ◽  
Clinical Testing ◽  
Multiplex Detection ◽  
Gene Detection ◽  
Microrna Regulation ◽  
Detection Assay ◽  
Cost Efficient ◽  
High Throughput Manner

Here, we have developed a set of fluorophore-labeled microspheres named rainbowarray microspheres. Based on the spectrally encoded microspheres, we further developed a liquid hybridization approach for multiplex target detection. Different from the prototype Luminex xMAP array, this technology enables feasible, flexible, and cost-efficient microsphere labeling and multiplex detection in a timely and high-throughput manner. To demonstrate the practicability of this technology, quantitative measurement of microRNA regulation was performed during the differentiation of 3T3-L1 cells, in which the expression of two microRNAs was determined at a 2 h interval during a process of 2 days. The flexibility and the timely and high-throughput properties of the technology enable it to be widely implemented in clinical testing.

Technological advances for interrogating the human kinome

2017 ◽  
Vol 45 (1) ◽  
pp. 65-77 ◽  
Author(s):  
Akanksha Baharani ◽  
Brett Trost ◽  
Anthony Kusalik ◽  
Scott Napper
Keyword(s):  
High Throughput ◽  
Therapeutic Intervention ◽  
Therapeutic Efficacy ◽  
Kinase Inhibitors ◽  
Cost Effective ◽  
Disease Prognosis ◽  
Technological Advances ◽  
Human Kinome ◽  
High Throughput Manner

There is increasing appreciation among researchers and clinicians of the value of investigating biology and pathobiology at the level of cellular kinase (kinome) activity. Kinome analysis provides valuable opportunity to gain insights into complex biology (including disease pathology), identify biomarkers of critical phenotypes (including disease prognosis and evaluation of therapeutic efficacy), and identify targets for therapeutic intervention through kinase inhibitors. The growing interest in kinome analysis has fueled efforts to develop and optimize technologies that enable characterization of phosphorylation-mediated signaling events in a cost-effective, high-throughput manner. In this review, we highlight recent advances to the central technologies currently available for kinome profiling and offer our perspectives on the key challenges remaining to be addressed.

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