Non-invasive chemical assessment of living human spermatozoa

RSC Advances ◽  
2015 ◽  
Vol 5 (14) ◽  
pp. 10424-10429 ◽  
Author(s):  
Eugen Edengeiser ◽  
Konrad Meister ◽  
Erik Bründermann ◽  
Steffen Büning ◽  
Simon Ebbinghaus ◽  
...  
Keyword(s):  
Raman Microspectroscopy ◽  
Human Spermatozoa ◽  
Physiological Conditions ◽  
Non Invasive ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman ◽  
Chemical Assessment

Confocal Raman microspectroscopy was used to chemically image single and living human spermatozoa under near-physiological conditions.

Confocal Raman microspectroscopy as an analytical tool to assess the mitochondrial status in human spermatozoa

The Analyst ◽  
2010 ◽  
Vol 135 (6) ◽  
pp. 1370 ◽  
Author(s):  
Konrad Meister ◽  
Diedrich A. Schmidt ◽  
Erik Bründermann ◽  
Martina Havenith
Keyword(s):  
Raman Microspectroscopy ◽  
Analytical Tool ◽  
Human Spermatozoa ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman

scholarly journals Current views on non-invasive in vivo determination of physiological parameters of the stratum corneum using confocal Raman microspectroscopy

2022 ◽  
Author(s):  
Maxim E. Darvin ◽  
Johannes Schleusener ◽  
Jürgen Lademann ◽  
Chun-Sik Choe
Keyword(s):  
Stratum Corneum ◽  
Raman Microspectroscopy ◽  
Physiological Parameters ◽  
Skin Barrier Function ◽  
Non Invasive ◽  
Intercellular Lipids ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman

Confocal Raman microspectroscopy is widely used in dermatology and cosmetology for analysis of the concentration of skin components (lipids, natural moisturizing factor molecules, water) and the penetration depth of cosmetic/medical formulations in the human stratum corneum (SC) in vivo. In recent years, it was shown that confocal Raman microspectroscopy can also be used for non-invasive in vivo depth-dependent determination of the physiological parameters of the SC, such as lamellar and lateral organization of intercellular lipids, folding properties of keratin, water mobility and hydrogen bonding states. The results showed that the strongest skin barrier function, which is primarily manifested by the orthorhombic organization of intercellular lipids, is provided at ≈20–40% SC depth, which is related to the maximal bonding state of water with surrounding components in the SC. The secondary and tertiary structures of keratin determine water binding in the SC, which is depth-dependent. This paper shows the technical possibility and advantage of confocal Raman microspectroscopy in non-invasive investigation of the skin and summarizes recent results on in vivo investigation of the human SC.

scholarly journals Molecular Composition Of Organelles In A Living Yeast Cell Studied By Confocal Raman Microspectroscopy

2003 ◽  
Vol 9 (S02) ◽  
pp. 1082-1083
Author(s):  
Yu-San Huang ◽  
Takeshi Karashima ◽  
Masayuki Yamamoto ◽  
Hiro-o Hamaguchi
Keyword(s):  
Yeast Cell ◽  
Raman Microspectroscopy ◽  
Molecular Composition ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman

Assessment of the “Skin Reservoir” of Urea by Confocal Raman Microspectroscopy and Reverse Iontophoresis in vivo

2007 ◽  
Vol 24 (10) ◽  
pp. 1897-1901 ◽  
Author(s):  
Valentine Wascotte ◽  
Peter Caspers ◽  
Johanna de Sterke ◽  
Michel Jadoul ◽  
Richard H. Guy ◽  
...  
Keyword(s):  
Raman Microspectroscopy ◽  
Reverse Iontophoresis ◽  
Skin Reservoir ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman

Correction of optical distortions in dry depth profiling with confocal Raman microspectroscopy

2011 ◽  
Vol 42 (6) ◽  
pp. 1330-1334 ◽  
Author(s):  
J. Pablo Tomba ◽  
María de la Paz Miguel ◽  
Claudio J. Perez
Keyword(s):  
Depth Profiling ◽  
Raman Microspectroscopy ◽  
Confocal Raman Microspectroscopy ◽  
Confocal Raman
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