Multifunctional biodegradable terbium-doped calcium phosphate nanoparticles: facile preparation, pH-sensitive drug release and in vitro bioimaging

RSC Advances ◽  
2014 ◽  
Vol 4 (95) ◽  
pp. 53122-53129 ◽  
Author(s):  
Han-Bing Shang ◽  
Feng Chen ◽  
Jin Wu ◽  
Chao Qi ◽  
Bing-Qiang Lu ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Drug Release ◽  
Ph Sensitive ◽  
Calcium Phosphate Nanoparticles ◽  
Facile Preparation

scholarly journals Calcium phosphate phase transition in the presence of Aminosilane

2014 ◽  
Vol 70 (a1) ◽  
pp. C67-C67
Author(s):  
Babak Mostaghaci ◽  
Brigitta Loretz ◽  
Robert Haberkorn ◽  
Guido Kickelbick ◽  
Claus-Michael Lehr
Keyword(s):  
Phase Transition ◽  
Calcium Phosphate ◽  
Blood Compatibility ◽  
Transfection Efficiency ◽  
Hek293 Cells ◽  
Step Method ◽  
Calcium Phosphate Nanoparticles ◽  
Amine Groups ◽  
The Impact

Calcium phosphate has been the point of interest for in vitro gene delivery for many years because of its biocompatibility and straight forward application. However, there are some limitations regarding in vivo administration of these particles mostly because of vast agglomeration of the particles and lack of strong bond between the particles and pDNA. We introduced a simple single step method to functionalize calcium phosphate nanoparticles with Aminosilanes having a different number of amine groups. The nanoparticles were characterized chemically and structurally and their toxicity and interaction with pDNA were studied as well. Results revealed that different crystalline phase of calcium phosphate nanoparticles (Brushite and Hydroxyapatite) with a size below 150 nm were prepared, depending on conditions of synthesis and phase, each with a narrow size distribution. The aminosilane agents caused oriented nucleation and growth of crystallites and can decrease the pH for producing hydroxyapatite phase. The phenomenon could be revealed with the presence of anisotropy in the structure of synthesized hydroxyapatite. The number of amine groups in the Aminosilane agent could change the phase transition pH. Brushite particles revealed to have stronger interaction with pDNA mostly because of their higher positive surface charge. Both particles showed blood compatibility and negligible toxicity. Transfection experiment revealed the capability of both brushite and hydroxyapatite particles to transfect A549 and HEK293 cells. The new modified nanoparticles can be stored in a dried state and re-dispersed easily at the time of administration. Moreover, the transfection efficiency is higher in comparison with conventional calcium phosphate. This study showed the impact of presence and type of the modifying agent on the crystal structure and the amount of surface functionalization of nanoparticles, which in consequence influenced their interaction with cells.

scholarly journals Design, Development, and Optimization of Polymeric Based-Colonic Drug Delivery System of Naproxen

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Pooja Sharma ◽  
Anuj Chawla ◽  
Pravin Pawar
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Ph Sensitive ◽  
Time Dependent ◽  
Wet Granulation ◽  
Design Development ◽  
Caecal Content ◽  
S 100 ◽  
Eudragit S 100

The aim of present investigation deals with the development of time-dependent and pH sensitive press-coated tablets for colon specific drug delivery of naproxen. The core tablets were prepared by wet granulation method then press coated with hydroxypropyl cellulose (HPC) or Eudragit RSPO : RLPO mixture and further coated with Eudragit S-100 by dip immerse method. Thein vitrodrug release study was conducted in different dissolution media such as pH 1.2, 6.8, and 7.4 with or without rat caecal content to simulate GIT conditions. Surface morphology and cross-sectional view of the tablets were visualized by scanning electron microscopy (SEM). All prepared batches were in compliance with the pharmacopoeial standards. The tablets which are compression coated with HPC followed by Eudragit S-100 coated showed highestin vitrodrug release of 98.10% in presence of rat caecal content. The SEM of tablets suggested that the number of pores got increased in pH 7.4 medium followed by dissolution of coating layer. The tablets coat erosion study suggested that the lag time depends upon the coating concentrations of polymers. A time-dependent hydrophilic polymer and pH sensitive polymer based press-coated tablets of naproxen were promising delivery for colon targeting.

Schiff base-containing dextran nanogel as pH-sensitive drug delivery system of doxorubicin: Synthesis and characterization

2018 ◽  
Vol 33 (2) ◽  
pp. 170-181 ◽  
Author(s):  
Hongying Su ◽  
Wen Zhang ◽  
Yayun Wu ◽  
Xiaodong Han ◽  
Gang Liu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Schiff Base ◽  
Drug Release ◽  
Drug Delivery System ◽  
Delivery System ◽  
Ph Sensitive ◽  
Cell Uptake ◽  
Stimuli Responsive ◽  
Drug Release Profile

Stimuli-responsive hydrogels have been widely researched as carrier systems, due to their excellent biocompatibility and responsiveness to external physiologic environment factors. In this study, dextran-based nanogel with covalently conjugated doxorubicin (DOX) was developed via Schiff base formation using the inverse microemulsion technique. Since the Schiff base linkages are acid-sensitive, drug release profile of the DOX-loaded nanogel would be pH-dependent. In vitro drug release studies confirmed that DOX was released much faster under acidic condition (pH 2.0, 5.0) than that at pH 7.4. Approximately 66, 28, and 9% of drug was released in 72 h at pH 2.0, 5.0, and 7.4, respectively. Cell uptake by the human breast cancer cell (MCF-7) demonstrated that the DOX-loaded dextran nanogel could be internalized through endocytosis and distributed in endocytic compartments inside tumor cells. These results indicated that the Schiff base-containing nanogel can serve as a pH-sensitive drug delivery system. And the presence of multiple aldehyde groups on the nanogel are available for further conjugations of targeting ligands or imaging probes.

Genetic immunization against hepatitis B virus with calcium phosphate nanoparticles in vitro and in vivo

2020 ◽  
Vol 110 ◽  
pp. 254-265
Author(s):  
Leonardo Rojas-Sánchez ◽  
Ejuan Zhang ◽  
Viktoriya Sokolova ◽  
Maohua Zhong ◽  
Hu Yan ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Calcium Phosphate ◽  
Hepatitis B ◽  
Genetic Immunization ◽  
B Virus ◽  
Calcium Phosphate Nanoparticles

Glucosamine-carrying temperature- and pH-sensitive microgels: Preparation, characterization, and in vitro drug release studies

2008 ◽  
Vol 322 (1) ◽  
pp. 333-341 ◽  
Author(s):  
Dayong Teng ◽  
Jingli Hou ◽  
Xinge Zhang ◽  
Xin Wang ◽  
Zhen Wang ◽  
...  
Keyword(s):  
Drug Release ◽  
Ph Sensitive ◽  
In Vitro Drug Release ◽  
Release Studies

Delivery of the TLR ligand poly(I:C) to liver cells in vitro and in vivo by calcium phosphate nanoparticles leads to a pronounced immunostimulation

2017 ◽  
Vol 64 ◽  
pp. 401-410 ◽  
Author(s):  
Viktoriya Sokolova ◽  
Zou Shi ◽  
Shunmei Huang ◽  
Yanqin Du ◽  
Mathis Kopp ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Liver Cells ◽  
Poly I:C ◽  
Calcium Phosphate Nanoparticles
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