Vesicular self-assembly of a natural triterpenoid arjunolic acid in aqueous medium: study of entrapment properties and in situ generation of gel–gold nanoparticle hybrid material

RSC Advances ◽  
2014 ◽  
Vol 4 (95) ◽  
pp. 53327-53334 ◽  
Author(s):  
Braja Gopal Bag ◽  
Rakhi Majumdar
Keyword(s):  
Aqueous Medium ◽  
Gold Nanoparticle ◽  
Hybrid Material ◽  
Self Assembly ◽  
Medium Study ◽  
Terminalia Arjuna ◽  
Saw Dust ◽  
Arjunolic Acid ◽  
In Situ Generation

Formation of vesicular gel and gel–gold nanoparticle hybrid material from arjunolic acid extractable from the saw-dust of Terminalia arjuna.

scholarly journals Endogenous microRNA Triggered Enzyme-free DNA Logic Self-assembly for Amplified Bioimaging and Enhanced Gene Therapy via in Situ Generation of siRNAs

2021 ◽  
Author(s):  
Qinghua Jiang ◽  
Shuzhen Yue ◽  
Kaixin Yu ◽  
Tian Tian ◽  
Jian Zhang ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Self Assembly ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Dna Amplification ◽  
High Sensitivity ◽  
Free Dna ◽  
Therapy Effect ◽  
In Situ Generation

Abstract BackgroundSmall interfering RNA (siRNA) has emerged as a kind of promising therapeutic agents for cancer therapy. However, the off-target effect and degradation are the main challenges for siRNAs delivery. Herein, an enzyme-free DNA amplification strategy initiated by a specific endogenous microRNA has been developed for in situ generation of siRNAs with enhanced gene therapy effect on cervical carcinoma.MethodsThis strategy contains three DNA hairpins (H1, H2/PS and H3) which can be triggered by microRNA-21 (miR-21) for self-assembly of DNA nanowheels (DNWs). Notably, this system is consistent with the operation of a DNA logic circuitry containing cascaded “AND” gates with feedback mechanism. Accordingly, a versatile biosensing and bioimaging platform is fabricated for sensitive and specific analysis of miR-21 in HeLa cells via fluorescence resonance energy transfer (FRET). Meanwhile, since the vascular endothelial growth factor (VEGF) antisense and sense sequences are encoded in hairpin reactants, the performance of this DNA circuit leads to in situ assembly of VEGF siRNAs in DNWs, which can be specifically recognized and cleaved by Dicer for gene therapy of cervical carcinoma. ResultsThe proposed isothermal amplification approach exhibits high sensitivity for miR-21 with a detection limit of 0.25 pM and indicates excellent specificity to discriminate target miR-21 from the single-base mismatched sequence. Furthermore, this strategy achieves accurate and sensitive imaging analysis of the expression and distribution of miR-21 in different living cells. To note, compared to naked siRNAs alone, in situ siRNA generation shows a significantly enhanced gene silencing and anti-tumor effect due to the high reaction efficiency of DNA circuit and improved delivery stability of siRNAs.ConclusionThe endogenous miRNA-activated DNA circuit provides an exciting opportunity to construct a general nanoplatform for precise cancer diagnosis and efficient gene therapy, which has an important significance in clinical translation.

Gold Nanoparticle Functionalized Artificial Nacre: Facile in Situ Growth of Nanoparticles on Montmorillonite Nanosheets, Self-Assembly, and Their Multiple Properties

ACS Nano ◽  
2012 ◽  
Vol 6 (9) ◽  
pp. 8250-8260 ◽  
Author(s):  
Hong-Bin Yao ◽  
Li-Bo Mao ◽  
You-Xian Yan ◽  
Huai-Ping Cong ◽  
Xuan Lei ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Self Assembly ◽  
In Situ Growth

scholarly journals Correction: Synthesis of well-defined α,ω-telechelic multiblock copolymers in aqueous medium: in situ generation of α,ω-diols

2017 ◽  
Vol 8 (47) ◽  
pp. 7416-7416 ◽  
Author(s):  
Alexandre Simula ◽  
Vasiliki Nikolaou ◽  
Athina Anastasaki ◽  
Fehaid Alsubaie ◽  
Gabit Nurumbetov ◽  
...  
Keyword(s):  
Aqueous Medium ◽  
Multiblock Copolymers ◽  
In Situ Generation

Correction for ‘Synthesis of well-defined α,ω-telechelic multiblock copolymers in aqueous medium: in situ generation of α,ω-diols’ by Alexandre Simula et al., Polym. Chem., 2015, 6, 2226–2233.

In situ Generation of Superoxide Anion Radical in Aqueous Medium under Ambient Conditions

ChemPhysChem ◽  
2013 ◽  
Vol 14 (18) ◽  
pp. 4158-4164 ◽  
Author(s):  
Uri Stoin ◽  
Alexander I. Shames ◽  
Itamar Malka ◽  
Ilana Bar ◽  
Yoel Sasson
Keyword(s):  
Aqueous Medium ◽  
Superoxide Anion ◽  
Anion Radical ◽  
Superoxide Anion Radical ◽  
Ambient Conditions ◽  
In Situ Generation

Selected-area in situ generation of highly fluorescent organic nanowires embedded in a polymer film: the solvent-vapor-induced self-assembly process

2010 ◽  
Vol 20 (36) ◽  
pp. 7715 ◽  
Author(s):  
Jong Won Chung ◽  
Byeong-Kwan An ◽  
Fumio Hirato ◽  
Jong H. Kim ◽  
Hiroshi Jinnai ◽  
...  
Keyword(s):  
Polymer Film ◽  
Self Assembly ◽  
Assembly Process ◽  
Solvent Vapor ◽  
In Situ Generation
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