Blending of cellulolytic enzyme preparations from different fungal sources for improved cellulose hydrolysis by increasing synergism

RSC Advances ◽  
2014 ◽  
Vol 4 (84) ◽  
pp. 44726-44732 ◽  
Author(s):  
Mukund Adsul ◽  
Bhawna Sharma ◽  
Reeta Rani Singhania ◽  
Jitendra Kumar Saini ◽  
Ankita Sharma ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Cellulose Hydrolysis ◽  
Cellulolytic Enzyme ◽  
Enzyme Preparations ◽  
Fungal Enzyme ◽  
Enzyme Cocktail ◽  
Different Types ◽  
Hydrolysis Of

A prepared enzyme cocktail from different fungal enzyme preparations increases the hydrolysis of avicel/wheat straw by increasing synergism between the same or different types of cellulases.

scholarly journals Defining the Frontiers of Synergism between Cellulolytic Enzymes for Improved Hydrolysis of Lignocellulosic Feedstocks

Catalysts ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1343
Author(s):  
Mpho S. Mafa ◽  
Brett I. Pletschke ◽  
Samkelo Malgas
Keyword(s):  
Value Added ◽  
Product Yield ◽  
Cellulose Hydrolysis ◽  
Cellulolytic Enzymes ◽  
Cellulolytic Enzyme ◽  
Key Factors ◽  
Cellulose Conversion ◽  
Lignocellulosic Feedstocks ◽  
Polysaccharide Monooxygenases ◽  
Hydrolysis Of

Lignocellulose has economic potential as a bio-resource for the production of value-added products (VAPs) and biofuels. The commercialization of biofuels and VAPs requires efficient enzyme cocktail activities that can lower their costs. However, the basis of the synergism between enzymes that compose cellulolytic enzyme cocktails for depolymerizing lignocellulose is not understood. This review aims to address the degree of synergism (DS) thresholds between the cellulolytic enzymes and how this can be used in the formulation of effective cellulolytic enzyme cocktails. DS is a powerful tool that distinguishes between enzymes’ synergism and anti-synergism during the hydrolysis of biomass. It has been established that cellulases, or cellulases and lytic polysaccharide monooxygenases (LPMOs), always synergize during cellulose hydrolysis. However, recent evidence suggests that this is not always the case, as synergism depends on the specific mechanism of action of each enzyme in the combination. Additionally, expansins, nonenzymatic proteins responsible for loosening cell wall fibers, seem to also synergize with cellulases during biomass depolymerization. This review highlighted the following four key factors linked to DS: (1) a DS threshold at which the enzymes synergize and produce a higher product yield than their theoretical sum, (2) a DS threshold at which the enzymes display synergism, but not a higher product yield, (3) a DS threshold at which enzymes do not synergize, and (4) a DS threshold that displays anti-synergy. This review deconvolutes the DS concept for cellulolytic enzymes, to postulate an experimental design approach for achieving higher synergism and cellulose conversion yields.

scholarly journals Synergistic Enzyme Cocktail to Enhance Hydrolysis of Steam Exploded Wheat Straw at Pilot Scale

2018 ◽  
Vol 6 ◽  
Author(s):  
Ruchi Agrawal ◽  
Surbhi Semwal ◽  
Ravindra Kumar ◽  
Anshu Mathur ◽  
Ravi Prakash Gupta ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Pilot Scale ◽  
Enzyme Cocktail ◽  
Hydrolysis Of

scholarly journals Hydrolysis of galacto-oligosaccharides in soy molasses by α -galactosidases and invertase from Aspergillus terreus

2010 ◽  
Vol 53 (3) ◽  
pp. 719-729 ◽  
Author(s):  
Angélica Pataro Reis ◽  
Valéria Monteze Guimarães ◽  
Joana Gasperazzo Ferreira ◽  
José Humberto de Queiroz ◽  
Maria Goreti Almeida Oliveira ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Hydrophobic Interaction ◽  
Aspergillus Terreus ◽  
Gel Filtration ◽  
Enzyme Preparations ◽  
Optimum Ph ◽  
Soy Molasses ◽  
Hydrolysis Of

Two α -galactosidase (P1 and P2) and one invertase present in the culture of Aspergillus terreus grown on wheat straw for 168 h at 28ºC were partially purified by gel filtration and hydrophobic interaction chromatographies. Optimum pH and temperatures for P1, P2 and invertase preparations were 4.5-5.0, 5.5 and 4.0 and 60, 55 and 65ºC, respectively. The K M app for Ï� -nitrophenyl-α -D-galactopyranoside were 1.32 mM and 0.72 mM for P1 and P2, respectively, while the K M app value for invertase, using sacarose as a substrate was 15.66 mM. Enzyme preparations P1 and P2 maintained their activities after pre-incubation for 3 h at 50ºC and invertase maintained about 90% after 6 h at 55 ºC. P1 and P2 presented different inhibition sensitivities by Ag+, D-galactose, and SDS. All enzyme preparations hydrolyzed galacto-ologosaccharides present in soymolasses.

Delignification overmatches hemicellulose removal for improving hydrolysis of wheat straw using the enzyme cocktail from Aspergillus niger

2019 ◽  
Vol 274 ◽  
pp. 459-467 ◽  
Author(s):  
Jiahong Wang ◽  
Xuntong Chen ◽  
Chonlong Chio ◽  
Chun Yang ◽  
Erzheng Su ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Wheat Straw ◽  
Hemicellulose Removal ◽  
Enzyme Cocktail ◽  
Hydrolysis Of

scholarly journals Cellulolytic and xylanolytic enzyme complex of Penicillium funiculosum Thom

1970 ◽  
pp. 261-265
Author(s):  
O. M. Yurieva ◽  
A. P. Hryhanskyi ◽  
S. O. Syrchin ◽  
L. T. Nakonechna ◽  
A. K. Pavlychenko ◽  
...  
Keyword(s):  
Comparative Study ◽  
Wheat Straw ◽  
Cellulolytic Enzyme ◽  
Enzyme Complex ◽  
Penicillium Funiculosum ◽  
Additional Component ◽  
Soil P ◽  
Glucosidase Activity ◽  
Xylanolytic Enzyme ◽  
Hydrolysis Of

Aim. The aim of this research was a comparative study of β-glucosidase activity of endophytic and soil Penicillium funiculosum strains. Methods. β-Gucosidase activity was determined by hydrolysis of pNPG on 4th and 6th days of cultivation. Barcoding of P. funiculosum 16795 DNA was carried out with ITS1 and ITS4 primers. Results. Obtained data demonstrate the ability of endophytic and soil P. funiculosum strains to synthesize β-glucosidase activity and hydrolyze Na-CMC and wheat straw. Studied activities enhanced with increasing cultivation time of micromycetes. Endophytic isolates of P. funiculosum had higher β-glucosidase activities than soil ones. Conclusions. P. funiculosum strains, especially 16795, are promising for further research of β-glucosidase preparations as additional component to the T. reesei enzymes, as well as to create recombinant constructs for obtaining a balanced composition of cellulolytic enzyme complexes.Keywords: Penicillium funiculosum, β-glucosidase activity, endophytes, soil strains.

scholarly journals ENZYMATIC HYDROLYSIS OF SOLUBLE CELLULOSE DERIVATIVES AS MEASURED BY CHANGES IN VISCOSITY

1950 ◽  
Vol 33 (5) ◽  
pp. 601-628 ◽  
Author(s):  
Hillel S. Levinson ◽  
Elwyn T. Reese
Keyword(s):  
Enzymatic Hydrolysis ◽  
Intrinsic Viscosity ◽  
Reducing Sugars ◽  
Enzyme System ◽  
Molecular Size ◽  
Cellulose Hydrolysis ◽  
Reducing Sugar ◽  
Cellulose Derivatives ◽  
Enzyme Preparations ◽  
Hydrolysis Of

Observation of changes in fluidity is presented as a method for following the enzymatic hydrolysis of soluble cellulose derivatives. The activity of different cell-free enzyme preparations may be compared by this method, providing certain precautions are observed. In general, results obtained by use of the fluidity method are similar to those obtained using the reducing sugar technique, indicating that the same enzyme system is measured by the two methods. Changes in the DP of the substratum may be followed within certain limits of molecular size. Results indicate that a random splitting of CMC occurs during enzymatic hydrolysis, with a concomitant decrease in intrinsic viscosity and an increase in reducing sugars. Certain inadequacies of the cellulose-cellobiose-glucose theory, together with more recent findings, have led to the postulation of an alternate explanation of the mechanism of cellulose hydrolysis.

scholarly journals Enzymatic Activity of Some Industrially-Applied Cellulolytic Enzyme Preparations

2017 ◽  
Vol 24 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Katarzyna Dąbkowska ◽  
Monika Mech ◽  
Kamil Kopeć ◽  
Maciej Pilarek
Keyword(s):  
Enzymatic Activity ◽  
Lignocellulosic Biomass ◽  
Synergistic Action ◽  
Solid Wastes ◽  
Cellulolytic Enzyme ◽  
Enzyme Preparations ◽  
Essential Step ◽  
Rye Straw ◽  
Pre Treatment ◽  
Hydrolysis Of

Abstract Enzymatic hydrolysis is the essential step in the production of 2nd generation biofuels made from lignocellulosic biomass, i.e. agricultural or forestry solid wastes. The enzyme-catalysed robust degradation of cellulose and hemicellulose to monosaccharides requires the synergistic action of the independent types of highly-specific enzymes, usually offered as ready-to-use preparations. The basic aim of the study was to experimentally determine the enzymatic activity of two widely industrially-applied, commercially available cellulolytic enzyme preparations: (i) Cellic® CTec2 and (ii) the mixture of Celluclast® 1.5L and Novozyme 188, in the hydrolysis of pre-treated lignocellulosic biomass, i.e. (a) energetic willow and (b) rye straw, or untreated (c) cellulose paper as well, used as feedstocks. Before the hydrolysis, every kind of utilized lignocellulosic biomass was subjected to alkaline-based (10% NaOH) pre-treatment at high-temperature (121°C) and overpressure (0.1 MPa) conditions. The influence of the type of applied enzymes, as well as their concentration, on the effectiveness of hydrolysis was quantitatively evaluated, and finally the enzyme activities were determined for each of tested cellulolytic enzyme preparations.

Sign in / Sign up

Export Citation Format

Share Document