Surface modified PMMA nanoparticles with tunable drug release and cellular uptake

RSC Advances ◽  
2014 ◽  
Vol 4 (84) ◽  
pp. 44472-44479 ◽  
Author(s):  
Ridhima Juneja ◽  
Indrajit Roy
Keyword(s):  
Drug Release ◽  
Cellular Uptake ◽  
Surface Coating ◽  
Uptake Efficiency ◽  
Surface Modified ◽  
Cellular Uptake Efficiency

Differentialin vitrocellular uptake efficiency of fluorophore-loaded PMMA nanoparticles, with (a) different size, and (b) different surface coating.

scholarly journals The morphology and surface charge-dependent cellular uptake efficiency of upconversion nanostructures revealed by single-particle optical microscopy

2018 ◽  
Vol 9 (23) ◽  
pp. 5260-5269 ◽  
Author(s):  
Di Zhang ◽  
Lin Wei ◽  
Meile Zhong ◽  
Lehui Xiao ◽  
Hung-Wing Li ◽  
...  
Keyword(s):  
Surface Charge ◽  
Optical Microscopy ◽  
Cellular Uptake ◽  
Single Particle ◽  
Cellular Internalization ◽  
Uptake Efficiency ◽  
Size And Shape ◽  
Internalization Process ◽  
Cellular Uptake Efficiency

The cellular uptake efficiency of nanostructures has been demonstrated to be highly dependent on the surface charge, size and shape although the cellular internalization process is still far from being well-understood.

Membrane Microdomain Structures of Liposomes and Their Contribution to the Cellular Uptake Efficiency into HeLa Cells

2016 ◽  
Vol 13 (2) ◽  
pp. 369-378 ◽  
Author(s):  
Yoshinori Onuki ◽  
Yasuko Obata ◽  
Kumi Kawano ◽  
Hiromu Sano ◽  
Reina Matsumoto ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Hela Cells ◽  
Uptake Efficiency ◽  
Membrane Microdomain ◽  
Cellular Uptake Efficiency

scholarly journals Biocompatible carbon-doped MoSe2 nanoparticles as a highly efficient targeted agent for human renal cell carcinoma

RSC Advances ◽  
2019 ◽  
Vol 9 (20) ◽  
pp. 11567-11575 ◽  
Author(s):  
Li Gao ◽  
Yi-zeng Fan ◽  
Tao-hong Zhang ◽  
Hui-qiu Xu ◽  
Xian-ling Zeng ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cellular Uptake ◽  
Renal Cell ◽  
Human Renal Cell Carcinoma ◽  
Carbon Doped ◽  
Uptake Efficiency ◽  
High Viability ◽  
Targeted Agent ◽  
Cellular Uptake Efficiency

HK-2 cells have weak cellular uptake efficiency leading to high viability with carbon-doped MoSe2 nanoparticles.

scholarly journals Development and Characterization of Nanoliposomal Hydroxyurea Against BT-474 Breast Cancer Cells

2019 ◽  
Vol 10 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Azam Akbari ◽  
Azim Akbarzadeh ◽  
Morteza Rafiee Tehrani ◽  
Reza Ahangari Cohan ◽  
Mohsen Chiani ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Drug Release ◽  
Zeta Potential ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Breast Cancer Cells ◽  
Drug Carriers ◽  
Diffusion Method ◽  
Diphenyl Tetrazolium Bromide

Purpose: Hydroxyurea (HU) is a well-known chemotherapy drug with several side effects which limit its clinical application. This study was conducted to improve its therapeutic efficiency against breast cancer using liposomes as FDA-approved drug carriers. Methods: PEGylated nanoliposomes-containing HU (NL-HU) were made via a thin-film hydration method, and assessed in terms of zeta potential, size, morphology, release, stability, cellular uptake, and cytotoxicity. The particle size and zeta potential of NL-HU were specified by zeta-sizer. The drug release from liposomes was assessed by dialysis diffusion method. Cellular uptake was evaluated by flow cytometry. The cytotoxicity was designated by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) test. Results: The size and zeta value of NL-HU were gotten as 85 nm and -27 mV, respectively. NL-HU were spherical.NL-HU vesicles were detected to be stable for two months. The slow drug release and Weibull kinetic model were obtained. Liposomes considerably enhanced the uptake of HU into BT-474 human breast cancer cells. The cytotoxicity of NL-HU on BT-474 cells was found to be significantly more than that of free HU. Conclusion: The results confirmed these PEGylated nanoliposomes containing drug are potentially suitable against in vitro model of breast cancer.

scholarly journals Effect of A 2-HP-β-Cyclodextrin Formulation on the Biological Transport and Delivery of Chemotherapeutic PLGA Nanoparticles

2020 ◽  
Author(s):  
Kangyu Zheng ◽  
Zeju Huang ◽  
Jiaying Huang ◽  
Xiangmei Liu ◽  
JUNFENG BAN ◽  
...  
Keyword(s):  
Drug Release ◽  
Cellular Uptake ◽  
Entrapment Efficiency ◽  
Plga Nanoparticles ◽  
Human Lung Cancer ◽  
Pharmacokinetic Analysis ◽  
Biological Transport ◽  
Sustained Drug Release

Abstract Background: The aim of this work was to develop a novel and feasible modification strategy by utilizing the supramolecular effect of 2-hydroxypropyl-beta-cyclodexrin (2-HP-β-CD) for enhancing the biological transport efficiency of paclitaxel (PTX)-loaded poly(lactide-co-glycolide)(PLGA) nanoparticles.Methods: PTX-loaded 2-HP-β-CD-modified PLGA nanoparticles (2-HP-β-CD/PLGA NPs) were prepared using the modified emulsion method. Nano-characteristics, drug release behavior, in vitro cytotoxicity, cellular uptake profiles and in vivo bio-behavior of the nanoparticles were then characterized. Results: Compared with the plain PLGA NPs, 2-HP-β-CD/PLGA NPs exhibited smaller particle sizes (151.03±1.36 nm), increased entrapment efficiency (~49.12% increase) and sustained drug release. When added to A549 human lung cancer cells, compared with PLGA NPs, 2-HP-β-CD/PLGA NPs exhibited higher cytotoxicity in MTT assays and improved cellular uptake efficiency. Pharmacokinetic analysis showed that the AUC value of 2-HP-β-CD/PLGA NPs was 2.4-fold higher than commercial Taxol® and 1.7-fold higher than plain PLGA NPs. In biodistribution assays, 2-HP-β-CD/PLGA NPs exhibited excellent stability in the circulation.Conclusions: The results of this study suggest that formulation contains 2-HP-β-CD can prolong PTX release, enhance drug transpot efficiency and serve as a potential tumor targeting system for PTX.

scholarly journals Folate Receptor-Targeting and Reactive Oxygen Species-Responsive Liposomal Formulation of Methotrexate for Treatment of Rheumatoid Arthritis

Pharmaceutics ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 582 ◽  
Author(s):  
Chen ◽  
Amerigos J.C. ◽  
Su ◽  
Guissi ◽  
Xiao ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Drug Release ◽  
Cellular Uptake ◽  
Folate Receptor ◽  
Oxygen Species ◽  
Activated Macrophages

Multifunctional nanomedicines with active targeting and stimuli-responsive drug release function utilizing pathophysiological features of the disease are regarded as an effective strategy for treatment of rheumatoid arthritis (RA). Under the inflammatory environment of RA, activated macrophages revealed increased expression of folate receptor and elevated intracellular reactive oxygen species (ROS) level. In this study, we successfully conjugated folate to polyethylene glycol 100 monostearate as film-forming material and further prepared methotrexate (MTX) and catalase (CAT) co-encapsulated liposomes, herein, shortened to FOL-MTX&CAT-L, that could actively target to activated macrophages. Thereafter, elevated intracellular hydrogen peroxide, the main source of ROS, diffused into liposomes and encapsulated CAT catalyzed the decomposition of hydrogen peroxide into oxygen and water. Continuous oxygen-generation inside liposomes would eventually disorganize its structure and release the encapsulated MTX. We characterized the in vitro drug release, cellular uptake and cytotoxicity studies as well as in vivo pharmacokinetics, biodistribution, therapeutic efficacy and safety studies of FOL-MTX&CAT-L. In vitro results revealed that FOL-MTX&CAT-L possessed sufficient ROS-sensitive drug release, displayed an improved cellular uptake through folate-mediated endocytosis and exhibited a higher cytotoxic effect on activated RAW264.7 cells. Moreover, in vivo results showed prolonged blood circulation time of PEGylated liposomes, enhanced accumulation of MTX in inflamed joints of collagen-induced arthritis (CIA) mice, reinforced therapeutic efficacy and minimal toxicity toward major organs. These results imply that FOL-MTX&CAT-L may be used as an effective nanomedicine system for RA treatment.

scholarly journals Comparison of Polydopamine-Coated Mesoporous Silica Nanorods and Spheres for the Delivery of Hydrophilic and Hydrophobic Anticancer Drugs

2019 ◽  
Vol 20 (14) ◽  
pp. 3408 ◽  
Author(s):  
Anna-Karin Pada ◽  
Diti Desai ◽  
Kaiyao Sun ◽  
Narayana Prakirth Govardhanam ◽  
Kid Törnquist ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Mesoporous Silica ◽  
Cellular Uptake ◽  
Drug Delivery Systems ◽  
Mesoporous Silica Nanoparticles ◽  
Drug Distribution ◽  
Delivery Systems ◽  
Hydrophilic Drugs

Mesoporous silica nanoparticles (MSNs) have been widely studied as drug delivery systems in nanomedicine. Surface coating of MSNs have enabled them to perform efficiently in terms of bioavailability, biocompatibility, therapeutic efficacy and targeting capability. Recent studies have suggested the use of polydopamine (PDA) as a facilitative coating for MSNs that provides sustained and pH-responsive drug release, owing to the adhesive “molecular-glue” function of PDA. This further endows these hybrid MSN@PDA particles with the ability to carry large amounts of hydrophilic drugs. In this study, we expand the feasibility of this platform in terms of exploring its ability to also deliver hydrophobic drugs, as well as investigate the effect of particle shape on intracellular delivery of both a hydrophilic and hydrophobic anticancer drug. MSN@PDA loaded with doxorubicin (hydrophilic) and fingolimod (hydrophobic) was studied via a systematic in vitro approach (cellular internalization, intracellular drug distribution and cytotoxicity). To promote the cellular uptake of the MSN@PDA particles, they were further coated with a polyethylene imine (PEI)-polyethylene glycol (PEG) copolymer. Drug-loaded, copolymer-coated MSN@PDA showed effective cellular uptake, intracellular release and an amplified cytotoxic effect with both doxorubicin and fingolimod. Additionally, rods exhibited delayed intracellular drug release and superior intracellular uptake compared to spheres. Hence, the study provides an example of how the choice and design of drug delivery systems can be tuned by the need for performance, and confirms the PDA coating of MSNs as a useful drug delivery platform beyond hydrophilic drugs.

Linseed polysaccharides based nanoparticles for controlled delivery of docetaxel: Design, in vitro drug release and cellular uptake

2019 ◽  
Vol 49 ◽  
pp. 143-151 ◽  
Author(s):  
Muhammad Tahir Haseeb ◽  
Nisar Ul Khaliq ◽  
Soon Hong Yuk ◽  
Muhammad Ajaz Hussain ◽  
Sajid Bashir
Keyword(s):  
Drug Release ◽  
Cellular Uptake ◽  
Controlled Delivery ◽  
In Vitro Drug Release
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