scholarly journals Paper-based molecular diagnostic for Chlamydia trachomatis

RSC Advances ◽  
2014 ◽  
Vol 4 (80) ◽  
pp. 42245-42251 ◽  
Author(s):  
Jacqueline C. Linnes ◽  
Andy Fan ◽  
Natalia M. Rodriguez ◽  
Bertrand Lemieux ◽  
Huimin Kong ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Point Of Care ◽  
Molecular Diagnostic ◽  
Rapid Diagnostics ◽  
Molecular Assay

The paper-based molecular assay can be performed at the point of care and is 100× more sensitive than current rapid diagnostics forchlamydiadetection.

scholarly journals Reduced sensitivity from pooled urine, pharyngeal and rectal specimens when using a molecular assay for the detection of chlamydia and gonorrhoea near the point of care

Sexual Health ◽  
2020 ◽  
Vol 17 (1) ◽  
pp. 15 ◽  
Author(s):  
Steven G. Badman ◽  
Sara F. E. Bell ◽  
Judith A. Dean ◽  
Jime Lemoire ◽  
Luke Coffey ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Molecular Detection ◽  
Point Of Care ◽  
Molecular Assay ◽  
Test Sensitivity ◽  
At Risk Populations ◽  
Individual Specimen ◽  
Overall Performance ◽  
Sex With Men ◽  
The Individual

Background The aim of this study was to compare the performance of pooled self-collected urogenital, pharyngeal and anorectal specimens to that of individual specimen results for the molecular detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) near the point of care (POC) for diagnostic sensitivity. Methods: Clients (mostly men who have sex with men) attending an urban community testing service and three sex-on-premises venues in Brisbane, Australia, were offered CT and NG testing by trained lay providers. Participants provided three self-collected specimens (urine, pharyngeal and rectal) for testing by GeneXpert (Cepheid, Sunnyvale, CA, USA). If any of the individual specimens from a participant were positive, all three specimens were pooled and retested. Results: Of the 388 participants who provided three individual anatomical specimens, 76 (19.6%) were found to be positive for CT and/or NG at one or more sites. The pooling approach failed to detect five CT rectal and four NG pharyngeal infections. The overall performance (sensitivity) of the pooling approach compared with individual specimen testing and Cohen’s κ were 90.0% and 0.86 respectively for CT and 89.7% and 0.89 respectively for NG. Conclusions: Reduced sensitivity was observed when using pooled specimens for the detection of CT and NG using GeneXpert near the POC, similar to results reported in laboratory-based CT and NG pooling studies. These data suggest specimen pooling is feasible near to the POC, potentially saving time and costs when screening at-risk populations for CT and NG. Our data also suggest a reduction in pooled urine could improve overall test sensitivity.

Molecular diagnostic of toxigenic Corynebacterium diphtheriae strain by DNA sensor potentially suitable for electrochemical point-of-care diagnostic

Talanta ◽  
2021 ◽  
Vol 227 ◽  
pp. 122161
Author(s):  
Kasper Marchlewicz ◽  
Iga Ostrowska ◽  
Sławomir Oszwałdowski ◽  
Aleksandra Zasada ◽  
Robert Ziółkowski ◽  
...  
Keyword(s):  
Point Of Care ◽  
Corynebacterium Diphtheriae ◽  
Molecular Diagnostic ◽  
Dna Sensor

scholarly journals Modular development of a prototype point of care molecular diagnostic platform for sexually transmitted infections

2016 ◽  
Vol 38 (8) ◽  
pp. 741-748 ◽  
Author(s):  
Manoharanehru Branavan ◽  
Ruth E. Mackay ◽  
Pascal Craw ◽  
Angel Naveenathayalan ◽  
Jeremy C. Ahern ◽  
...  
Keyword(s):  
Sexually Transmitted Infections ◽  
Point Of Care ◽  
Molecular Diagnostic ◽  
Sexually Transmitted

Diagnostic tests for detecting Chlamydia trachomatis and Neisseria gonorrhoeae in rectal and pharyngeal specimens

2021 ◽  
Author(s):  
Paul C. Adamson ◽  
Jeffrey D. Klausner
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Bacterial Infections ◽  
Point Of Care ◽  
High Sensitivity ◽  
Population Level ◽  
The United States ◽  
Nucleic Acid Amplification ◽  
Point Of Care Test ◽  
Control And Prevention

Chlamydia trachomatis and Neisseria gonorrhoeae are two of the most often reported bacterial infections in the United States. The rectum and oropharynx are important anatomic sites of infection and can contribute to ongoing transmission. Nucleic acid amplification tests (NAATs) are the mainstays for the detection of C. trachomatis and N. gonorrhoeae infections owing to their high sensitivity and specificity. Several NAATs have been evaluated for testing in rectal and pharyngeal infections. A few assays recently received clearance by the Food and Drug Administration, including one point-of-care test. Those assays can be used for testing in symptomatic individuals, as well as for asymptomatic screening in certain patient populations. Routine screening for C. trachomatis in pharyngeal specimens is not recommended by the Centers for Disease Control and Prevention, though is often performed due to the use of multiplex assays. While expanding the types of settings for screening and using self-collected rectal and pharyngeal specimens can help to increase access and uptake of testing, additional research is needed to determine the potential benefits and costs associated with increased screening for rectal and pharyngeal C. trachomatis and N. gonorrhoeae infections on a population level.

Point-of-care molecular diagnostic devices: an overview

2015 ◽  
Vol 16 (12) ◽  
pp. 1399-1409 ◽  
Author(s):  
Chi Lan Nguyen Vu ◽  
Jianxiong Chan ◽  
Marian Todaro ◽  
Stan Skafidas ◽  
Patrick Kwan
Keyword(s):  
Point Of Care ◽  
Molecular Diagnostic

A point-of-care diagnostic for differentiating Ebola from endemic febrile diseases

2018 ◽  
Vol 10 (471) ◽  
pp. eaat0944 ◽  
Author(s):  
David Sebba ◽  
Alexander G. Lastovich ◽  
Melody Kuroda ◽  
Eric Fallows ◽  
Joshua Johnson ◽  
...  
Keyword(s):  
Ebola Virus ◽  
Clinical Symptoms ◽  
Point Of Care ◽  
Hemorrhagic Fever ◽  
Diagnostic Methods ◽  
Outbreak Detection ◽  
Molecular Diagnostic ◽  
Clinical Samples ◽  
Live Virus ◽  
And Control

Hemorrhagic fever outbreaks such as Ebola are difficult to detect and control because of the lack of low-cost, easily deployable diagnostics and because initial clinical symptoms mimic other endemic diseases such as malaria. Current molecular diagnostic methods such as polymerase chain reaction require trained personnel and laboratory infrastructure, hindering diagnostics at the point of need. Although rapid tests such as lateral flow can be broadly deployed, they are typically not well-suited for differentiating among multiple diseases presenting with similar symptoms. Early detection and control of Ebola outbreaks require simple, easy-to-use assays that can detect and differentiate infection with Ebola virus from other more common febrile diseases. Here, we developed and tested an immunoassay technology that uses surface-enhanced Raman scattering (SERS) tags to simultaneously detect antigens from Ebola, Lassa, and malaria within a single blood sample. Results are provided in <30 min for individual or batched samples. Using 190 clinical samples collected from the 2014 West African Ebola outbreak, along with 163 malaria positives and 233 negative controls, we demonstrated Ebola detection with 90.0% sensitivity and 97.9% specificity and malaria detection with 100.0% sensitivity and 99.6% specificity. These results, along with corresponding live virus and nonhuman primate testing of an Ebola, Lassa, and malaria 3-plex assay, indicate the potential of the SERS technology as an important tool for outbreak detection and clinical triage in low-resource settings.

scholarly journals Innovative point-of-care molecular diagnostic test for COVID-19 in India

2020 ◽  
Vol 1 (7) ◽  
pp. e277
Author(s):  
Nivedita Gupta ◽  
Salaj Rana ◽  
Harpreet Singh
Keyword(s):  
Diagnostic Test ◽  
Point Of Care ◽  
Molecular Diagnostic ◽  
Molecular Diagnostic Test

scholarly journals Smart cup: A minimally-instrumented, smartphone-based point-of-care molecular diagnostic device

2016 ◽  
Vol 229 ◽  
pp. 232-238 ◽  
Author(s):  
Shih-Chuan Liao ◽  
Jing Peng ◽  
Michael G. Mauk ◽  
Sita Awasthi ◽  
Jinzhao Song ◽  
...  
Keyword(s):  
Point Of Care ◽  
Molecular Diagnostic ◽  
Diagnostic Device
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