Extraction of alpha-fetoprotein (AFP) with magnetic albuminoid-imprinted poly(ethylene-co-vinyl alcohol) nanoparticles from human hepatocellular carcinoma HepG2 cellular culture medium

RSC Advances ◽  
2014 ◽  
Vol 4 (70) ◽  
pp. 36990-36995 ◽  
Author(s):  
Mei-Hwa Lee ◽  
Arti Ahluwalia ◽  
Ke-Ming Hsu ◽  
Wei-Ti Chin ◽  
Hung-Yin Lin
Keyword(s):  
Hepatocellular Carcinoma ◽  
Vinyl Alcohol ◽  
Culture Medium ◽  
Selective Adsorption ◽  
Alpha Fetoprotein ◽  
Human Hepatocellular Carcinoma ◽  
Poly Ethylene ◽  
Cellular Culture

The selective adsorption of alpha-fetoprotein using albuminoid MMIP nanoparticles from the human hepatocellular carcinoma HepG2 cellular culture medium.

Radiolabelled monoclonal antibodies against alpha-fetoprotein for in vivo localization of human hepatocellular carcinoma by immunotomoscintigraphy

1987 ◽  
Vol 13 (8) ◽  
pp. 385-390 ◽  
Author(s):  
Jean-Fran�ois Bergmann ◽  
Jean-Denis Lumbroso ◽  
Luc Manil ◽  
Jean-Claude Saccavini ◽  
Philippe Rougier ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Monoclonal Antibodies ◽  
Alpha Fetoprotein ◽  
Human Hepatocellular Carcinoma ◽  
Radiolabelled Monoclonal Antibodies

scholarly journals Estimation of Leucine Aminopeptidase and 5-Nucleotidase Increases Alpha-Fetoprotein Sensitivity in Human Hepatocellular Carcinoma Cases

2015 ◽  
Vol 16 (3) ◽  
pp. 959-963 ◽  
Author(s):  
Mekky Mohammed Abouzied ◽  
Heba M. Eltahir ◽  
Michael Atef Fawzy ◽  
Nabil Mohie Abdel-Hamid ◽  
Amany Saber Gerges ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Leucine Aminopeptidase ◽  
Alpha Fetoprotein ◽  
Human Hepatocellular Carcinoma

scholarly journals Regulation Effect of Zinc Fingers and Homeoboxes 2 on Alpha-Fetoprotein in Human Hepatocellular Carcinoma

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Shao Wei Hu ◽  
Meng Zhang ◽  
Ling Xue ◽  
Jian Ming Wen
Keyword(s):  
Hepatocellular Carcinoma ◽  
Mrna Expression ◽  
Hepg2 Cells ◽  
Zinc Fingers ◽  
Alpha Fetoprotein ◽  
Human Hepatocellular Carcinoma ◽  
Strong Positive Correlation ◽  
Nuclear Factor ◽  
Protein Levels ◽  
Serum Alpha Fetoprotein

Aim. To investigate the relationship between alpha-fetoprotein and zinc fingers and homeoboxes 2 in hepatocellular carcinoma.Materials and Methods. The expressions of zinc fingers and homeoboxes 2, nuclear factor-YA, and alpha-fetoprotein mRNA in 63 hepatocellular carcinoma were detected by reverse transcriptase-polymerase chain reaction and compared with the clinical parameters of the patients. Selectively, silence of zinc fingers and homeoboxes 2 in HepG2 cells was detected by RNA interference technique.Results. Alpha-fetoprotein mRNA expression was detected in 60.3% of hepatocellular carcinoma cases. Zinc fingers and homeoboxes 2 mRNA expression (36.5%) was significantly negatively correlated with serum alpha-fetoprotein concentration and mRNA expression. A strong positive correlation was found between zinc fingers and homeoboxes 2 and nuclear factor-YA mRNA expression (42.9%), while the latter was negatively correlated with serum alpha-fetoprotein concentration and mRNA expression. Treatment with zinc fingers and homeoboxes 2 small interfering RNA led to 85% and 83% silence of zinc fingers and homeoboxes 2 mRNA and protein expression and 60% and 61% reduction of nuclear factor-YA mRNA and protein levels in the HepG2 cells, respectively. Downregulation of zinc fingers and homeoboxes 2 also induced a 2.4-fold increase in both alpha-fetoprotein mRNA and protein levels.Conclusions. Zinc fingers and homeoboxes 2 can regulate alpha-fetoprotein expression via the interaction with nuclear factor-YA in human hepatocellular carcinoma and may be used as an adjuvant diagnostic marker for alpha-fetoprotein-negative hepatocellular carcinoma.

Alpha-fetoprotein acts as a novel signal molecule and mediates transcription of Fn14 in human hepatocellular carcinoma

2012 ◽  
Vol 57 (2) ◽  
pp. 322-329 ◽  
Author(s):  
Shanshan Wang ◽  
Wei Jiang ◽  
Xiangmei Chen ◽  
Chao Zhang ◽  
Hui Li ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Alpha Fetoprotein ◽  
Human Hepatocellular Carcinoma ◽  
Signal Molecule
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