Synthesis, characterization and cytotoxicity of europium incorporated ZnO–graphene nanocomposites on human MCF7 breast cancer cells

RSC Advances ◽  
2014 ◽  
Vol 4 (71) ◽  
pp. 37479-37490 ◽  
Author(s):  
Susanta Bera ◽  
Monisankar Ghosh ◽  
Moumita Pal ◽  
Nilanjana Das ◽  
Suchandrima Saha ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser ◽  
Human Breast ◽  
Scanning Microscope ◽  
Graphene Nanocomposites ◽  
Graphene Nanocomposite

Eu incorporated ZnO–graphene nanocomposite in human breast cancer cells (MCF7) under a confocal laser scanning microscope.

scholarly journals Effect of HIT Components on the Development of Breast Cancer Cells

Life ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 832
Author(s):  
Li-Yu Chen ◽  
Gurunath Apte ◽  
Annerose Lindenbauer ◽  
Marion Frant ◽  
Thi-Huong Nguyen
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Laser Scanning ◽  
Cancer Metastasis ◽  
Cell Spreading ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Platelet Factor

Cancer cells circulating in blood vessels activate platelets, forming a cancer cell encircling platelet cloak which facilitates cancer metastasis. Heparin (H) is frequently used as an anticoagulant in cancer patients but up to 5% of patients have a side effect, heparin-induced thrombocytopenia (HIT) that can be life-threatening. HIT is developed due to a complex interaction among multiple components including heparin, platelet factor 4 (PF4), HIT antibodies, and platelets. However, available information regarding the effect of HIT components on cancers is limited. Here, we investigated the effect of these materials on the mechanical property of breast cancer cells using atomic force microscopy (AFM) while cell spreading was quantified by confocal laser scanning microscopy (CLSM), and cell proliferation rate was determined. Over time, we found a clear effect of each component on cell elasticity and cell spreading. In the absence of platelets, HIT antibodies inhibited cell proliferation but they promoted cell proliferation in the presence of platelets. Our results indicate that HIT complexes influenced the development of breast cancer cells.

scholarly journals Specific Intracellular Uptake of Herceptin-Conjugated CdSe/ZnS Quantum Dots into Breast Cancer Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Seung-Jin Han ◽  
Pierson Rathinaraj ◽  
Soo-Young Park ◽  
Young Kyoo Kim ◽  
Joon Hyung Lee ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Quantum Dots ◽  
Cell Death ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Laser Scanning ◽  
Specific Binding ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Core Shell

Herceptin, a typical monoclonal antibody, was immobilized on the surface of CdSe/ZnS core-shell quantum dots (QDs) to enhance their specific interactions with breast cancer cells (SK-BR3). The mean size of the core-shell quantum dots (28 nm), as determined by dynamic light scattering, increased to 86 nm after herceptin immobilization. Thein vitrocell culture experiment showed that the keratin forming cancer cells (KB) proliferated well in the presence of herceptin-conjugated QDs (QD-Her, 5 nmol/mL), whereas most of the breast cancer cells (SK-BR3) had died. To clarify the mechanism of cell death, the interaction of SK-BR3 cells with QD-Her was examined by confocal laser scanning microscopy. As a result, the QD-Her bound specifically to the membrane of SK-BR3, which became almost saturated after 6 hours incubation. This suggests that the growth signal of breast cancer cells is inhibited completely by the specific binding of herceptin to the Her-2 receptor of SK-BR3 membrane, resulting in cell death.

IMPORTANCE OF THE LASER SCANNING CYTOMETRY IN THE STUDY OF THE SITES OF INTERACTION OF HORMONES WITH THE HUMAN BREAST CANCER CELLS (MCF-7)

1991 ◽  
Vol 73 (2-3) ◽  
pp. 30a-30a
Author(s):  
Pierre German ◽  
Philippe Metezeau ◽  
Hélène Kiefer ◽  
Marie-Hélène Ratinaud ◽  
Gérard Habrioux
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Laser Scanning ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Laser Scanning Cytometry ◽  
Mcf 7

3-D imaging of cells using a confocal laser scanning microscope and digital image processing

1988 ◽  
Vol 46 ◽  
pp. 96-97
Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Biochemical Processes ◽  
Adjacent Structures

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.

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