Substituent effect on fluorescence signaling of the cell permeable HSO4− receptors through single point to ratiometric response in green solvent

RSC Advances ◽  
2014 ◽  
Vol 4 (53) ◽  
pp. 27665-27673 ◽  
Author(s):  
Manjira Mukherjee ◽  
Siddhartha Pal ◽  
Buddhadeb Sen ◽  
Somenath Lohar ◽  
Samya Banerjee ◽  
...  
Keyword(s):  
Substituent Effect ◽  
Single Point ◽  
Green Solvent ◽  
Effect Of Substituents ◽  
Biological Studies ◽  
Fluorescence Signaling

Effect of substituents on tuning of fluorescence signaling of the cell permeable HSO4− ions receptors through single point to ratiometric response in green solvent was systematically explored through analytical and biological studies.

Effect of substituents on absorption and fluorescence properties of pyrazolo[3,4-b]pyrrolo[2,3-d]pyridines

2008 ◽  
Vol 86 (11) ◽  
pp. 1070-1076 ◽  
Author(s):  
Bhausaheb K Ghotekar ◽  
Muddassar A Kazi ◽  
Madhukar N Jachak ◽  
Raghunath B Toche
Keyword(s):  
Good Yield ◽  
Substituent Effect ◽  
Photophysical Properties ◽  
Fluorescence Properties ◽  
Absorption And Emission ◽  
Effect Of Substituents ◽  
Convenient Route

A convenient route was successfully developed for the synthesis of novel heterocycles such as pyrazolo[3,4-b]pyrrolo[2,3-d]pyridines (PPP) from pyrazolo[3,4-b]pyridines in good yield. The PPP derivatives synthesized were further studied for their photophysical properties, and it was observed that absorption and emission λmax changed, owing to the substituent effect at 4 positions. These compounds were obtained from highly reactive starting materials, 5-aminopyrazoles and α-acetyl γ-butyrolactone.Key words: α-acetyl γ-butyrolactone, pyrazolo[3,4-b]pyrrolo[2,3-d] pyridine, absorption, emission, fluorescence.

Thermolysis of unsymmetrically substituted vinylcyclopropanes. The effect of substituents on the site-selectivity of homo-[1,5]-sigmatropic hydrogen migrations

1984 ◽  
Vol 62 (11) ◽  
pp. 2392-2395 ◽  
Author(s):  
Edward Piers ◽  
Anderson Richard Maxwell
Keyword(s):  
Substituent Effect ◽  
Sharp Contrast ◽  
Effect Of Substituents ◽  
Site Selectivity ◽  
Hydrogen Shifts

Thermolysis of the unsymmetrical vinylcyclopropanes 18–24 reveals a notable substituent effect on the site-selectivity of homo-[1,5]-sigmatropic hydrogen shifts. Rearrangement of 18–20 (OMe substituent) produces only products 25–27, respectively (HA migration). In sharp contrast, thermolysis of 24 (Me3Si substituent) provides exclusively substance 34 (HX migration). Thermolysis of substrates bearing methyl (21, 22) or phenyl (23) groups provide, in each case, mixtures of the two possible products.

Modification of the Electron Microscope for Investigation of Fully Hydrated Biological Specimens

1969 ◽  
Vol 27 ◽  
pp. 418-419 ◽  
Author(s):  
R. C. Moretz ◽  
D. F. Parsons
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Structural Damage ◽  
Lower Percentage ◽  
Vacuum Drying ◽  
Total Removal ◽  
Total Absence ◽  
Biological Studies ◽  
Electron Microscopes ◽  
Beam Damage

Short lifetime or total absence of electron diffraction of ordered biological specimens is an indication that the specimen undergoes extensive molecular structural damage in the electron microscope. The specimen damage is due to the interaction of the electron beam (40-100 kV) with the specimen and the total removal of water from the structure by vacuum drying. The lower percentage of inelastic scattering at 1 MeV makes it possible to minimize the beam damage to the specimen. The elimination of vacuum drying by modification of the electron microscope is expected to allow more meaningful investigations of biological specimens at 100 kV until 1 MeV electron microscopes become more readily available. One modification, two-film microchambers, has been explored for both biological and non-biological studies.

Design of Sensitive Photographic Materials for the High-Voltage Electron Microscope

1975 ◽  
Vol 33 ◽  
pp. 156-157
Author(s):  
Murray Vernon King ◽  
Donald F. Parsons
Keyword(s):  
Electron Microscope ◽  
Radiation Damage ◽  
High Voltage ◽  
Radiation Sensitivity ◽  
Fast Electrons ◽  
Voltage Electron ◽  
High Voltage Electron Microscope ◽  
High Voltage Electron ◽  
Biological Studies ◽  
Low Sensitivity

Effective application of the high-voltage electron microscope to a wide variety of biological studies has been restricted by the radiation sensitivity of biological systems. The problem of radiation damage has been recognized as a serious factor influencing the amount of information attainable from biological specimens in electron microscopy at conventional voltages around 100 kV. The problem proves to be even more severe at higher voltages around 1 MV. In this range, the problem is the relatively low sensitivity of the existing recording media, which entails inordinately long exposures that give rise to severe radiation damage. This low sensitivity arises from the small linear energy transfer for fast electrons. Few developable grains are created in the emulsion per electron, while most of the energy of the electrons is wasted in the film base.

Application of stereological analysis of cell volume to isolated myocytes in culture with and without adrenergic innervation

1990 ◽  
Vol 48 (3) ◽  
pp. 416-417
Author(s):  
Jane K. Rosenthal ◽  
Dianne L. Atkins ◽  
William J. Marvin ◽  
Penny A. Krumm
Keyword(s):  
Cardiac Myocytes ◽  
Structural Changes ◽  
Three Dimensional ◽  
Adrenergic Innervation ◽  
Culture Cell ◽  
Serial Sections ◽  
Newborn Rats ◽  
Primary Culture Cell ◽  
Biological Studies ◽  
Cell Volumes

To comprehend structural changes in cardiac myocytes accompanying adrenergic innervation, it is essential that a three dimensional analysis be performed. To date, biological studies which utilize stereological methods have been limited to cells in tissue and in organs. Our laboratory has utilized current stereological techniques for measuring absolute volumes of individual myocytes in primary culture. Cell volumes are calculated for two distinct groups of cells at 96 hours in culture: isolated myocytes and myocytes innervated with adrenergic neurons (Figure 1).Cardiac myocytes are cultured from the ventricular apices of newborn rats. Cells are plated directly onto tissue culture dishes with or without preplated explants from the paravertebral thoracolumbar sympathetic chain. On day four cultures are photographed and marked for one-to-one cell location. Following conventional fixation and embeddment in eponate-12, the cells are relocated and mounted for microtomy. The cells are completely sectioned at 120nm in their parallel orientation to the surface of the dish (Figure 2). Serial sections are collected on formvar coated slotted grids and are recorded in sequence.

Catechin derivatives from Parapiptadenia rigida: biological studies and conformational analysis

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
C Schmidt ◽  
M Fronza ◽  
R Murillo ◽  
V Wray ◽  
G Bringmann ◽  
...  
Keyword(s):  
Conformational Analysis ◽  
Biological Studies

Chemical and biological studies on the methanol extract of Gynura pseudochina var. hispida

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
N Siriwatanametanon ◽  
J Prieto ◽  
M Heinrich
Keyword(s):  
Methanol Extract ◽  
Biological Studies

Fine-scale taxonomic and temporal variability in the energy density of invertebrate prey of juvenile Chinook salmon Oncorhynchus tshawytscha

2020 ◽  
Vol 655 ◽  
pp. 185-198
Author(s):  
J Weil ◽  
WDP Duguid ◽  
F Juanes
Keyword(s):  
Energy Density ◽  
Chinook Salmon ◽  
Temporal Variability ◽  
Energy Content ◽  
Single Point ◽  
Single Species ◽  
Fine Scale ◽  
Temporal Differences ◽  
Juvenile Chinook Salmon ◽  
Juvenile Chinook

Variation in the energy content of prey can drive the diet choice, growth and ultimate survival of consumers. In Pacific salmon species, obtaining sufficient energy for rapid growth during early marine residence is hypothesized to reduce the risk of size-selective mortality. In order to determine the energetic benefit of feeding choices for individuals, accurate estimates of energy density (ED) across prey groups are required. Frequently, a single species is assumed to be representative of a larger taxonomic group or related species. Further, single-point estimates are often assumed to be representative of a group across seasons, despite temporal variability. To test the validity of these practices, we sampled zooplankton prey of juvenile Chinook salmon to investigate fine-scale taxonomic and temporal differences in ED. Using a recently developed model to estimate the ED of organisms using percent ash-free dry weight, we compared energy content of several groups that are typically grouped together in growth studies. Decapod megalopae were more energy rich than zoeae and showed family-level variability in ED. Amphipods showed significant species-level variability in ED. Temporal differences were observed, but patterns were not consistent among groups. Bioenergetic model simulations showed that growth rate of juvenile Chinook salmon was almost identical when prey ED values were calculated on a fine scale or on a taxon-averaged coarse scale. However, single-species representative calculations of prey ED yielded highly variable output in growth depending on the representative species used. These results suggest that the latter approach may yield significantly biased results.

Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple

2018 ◽  
Vol 39 (4) ◽  
pp. 474-482
Author(s):  
Hoang Thi Le Thuong ◽  
Nguyen Quang Hao ◽  
Tran Thi Thuy
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Ph ◽  
Yeast Strains ◽  
Biological Studies ◽  
Taxonomic Characterization

Eight yeast strains (denoted as D1 to D8) were isolated from samples of natural fermented pineapple. Strain D8 showed highest alcoholic production at low pH and special aroma of pineapple has been chosen for further study. Taxonomic characterization of strain D8 using morphological, biochemical and molecular biological studies confirmed that strain D8  belong to Saccharomycetaceae family, Saccharomycetales order and Saccharomyces cerevisiae species. Therefore, we named this strain as Saccharomyces cerevisiae D8 for further study on Brandy production from pineapple. Citation: Hoang Thi Le Thuong, Nguyen Quang Hao, Tran Thi Thuy, 2017. Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple. Tap chi Sinh hoc, 39(4): 474- 482. DOI: 10.15625/0866-7160/v39n4.10864.*Corresponding author: [email protected] Received 5 December 2016, accepted 12 August 2017

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