l-(+)-Lactic acid production by co-fermentation of cellobiose and xylose without carbon catabolite repression using Enterococcus mundtii QU 25

RSC Advances ◽  
2014 ◽  
Vol 4 (42) ◽  
pp. 22013-22021 ◽  
Author(s):  
Ying Wang ◽  
Mohamed Ali Abdel-Rahman ◽  
Yukihiro Tashiro ◽  
Yaotian Xiao ◽  
Takeshi Zendo ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lignocellulosic Biomass ◽  
Production System ◽  
Catabolite Repression ◽  
Acid Production ◽  
Carbon Catabolite Repression ◽  
Lactic Acid Production ◽  
Fed Batch ◽  
Bacterial Cultures ◽  
Enterococcus Mundtii

We established an effective highl-lactic acid production system based on fed-batch bacterial cultures utilising lignocellulosic biomass-derived mixed sugars without carbon catabolite repression.

scholarly journals Thermophilic Enterococcus faecium QU 50 enabled open repeated batch fermentation for l-lactic acid production from mixed sugars without carbon catabolite repression

RSC Advances ◽  
2017 ◽  
Vol 7 (39) ◽  
pp. 24233-24241 ◽  
Author(s):  
J. Tan ◽  
M. A. Abdel-Rahman ◽  
M. Numaguchi ◽  
Y. Tashiro ◽  
T. Zendo ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Enterococcus Faecium ◽  
Catabolite Repression ◽  
Acid Production ◽  
Batch Fermentation ◽  
Carbon Catabolite Repression ◽  
Lactic Acid Production ◽  
Acid Fermentation ◽  
Repeated Batch Fermentation ◽  
Repeated Batch

Thermophilic lactic acid bacterium enabled homo-l-lactic acid fermentation from hexose/pentose without carbon catabolite repression, and open repeated production by immobilization.

scholarly journals Lactic acid production from lime-treated wheat straw by Bacillus coagulans: neutralization of acid by fed-batch addition of alkaline substrate

2008 ◽  
Vol 78 (5) ◽  
pp. 751-758 ◽  
Author(s):  
Ronald H. W. Maas ◽  
Robert R. Bakker ◽  
Mickel L. A. Jansen ◽  
Diana Visser ◽  
Ed de Jong ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Wheat Straw ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Bacillus Coagulans ◽  
Fed Batch

scholarly journals Dynamic Simulation of Continuous Mixed Sugars Fermentation with Increasing Cell Retention Time for Lactic Acid Production using Enterococcus mundtii QU 25

2020 ◽  
Author(s):  
Ying Wang ◽  
Ka-Lai Chan ◽  
Mohamed Ali Abdel-Rahman ◽  
Kenji Sonomoto ◽  
Shao-Yuan Leu
Keyword(s):  
Lactic Acid ◽  
Retention Time ◽  
Acid Production ◽  
Fermentation Process ◽  
Lactic Acid Production ◽  
Critical Role ◽  
Cell Retention ◽  
Mixed Sugars ◽  
Enterococcus Mundtii ◽  
Mixed Sugar

Abstract Background: Simultaneous and effective conversion of both pentose and hexoses in fermentation is a critical and challenging task toward the lignocellulosic economy. This study aims to investigate the feasibility of an innovative co-fermentation process featured with cell recycle unit (CF/CR) for mixed sugar utilization. A l-lactic acid producing strain Enterococcus mundtii QU 25 was applied in the continuous fermentation process to utilize the mixed sugar at different productivities over the changes of flowing conditions. Structured numerical platform were constructed with the experiments to optimize the biological process and clarify the cell metabolisms through kinetics analysis. The structured model, kinetic parameters, and achievement of the fermentation strategy shall provide new insights towards whole sugar fermentation via real-time monitoring for process control and optimization.Results: Significant carbon catabolite repression of co-fermentation using glucose/xylose mixture was overcome by replacing glucose with cellobiose, of which the consumption ratio of hexose to pentose was improved dramatically from 10.4:1 to 2.17:1. An outstanding product concentration of 65.15 g·L -1 and productivity of 13.03 g·L -1 ·h -1 were achieved with 50 g·L -1 cellobiose and 30 g·L -1 xylose, at an optimized dilution rate of 0.2 h -1 with gradually increased cell retention time. Among the total lactic acid production, xylose contributed to more than 34% of the mixed sugars, which was close to the related contents in agricultural residuals. The model successfully simulated the transition of sugar consumption, cell growth, and lactic acid production among the batch, continuous process, and CF/CR system.Conclusion: Cell retention time played a critical role in balancing pentose and hexose consumption, cell decay, and lactic acid production in the CF/CR process. With the increase of cell concentration, consumption of mixed sugars increased with the productivity of final products, hence the impacts of substrate inhibiting reduced. With the validated parameters, the model showed highest accuracy simulating the CF/CR process, of which significantly longer cell retention times over hydraulic retention time were tested.

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