Nanoporous scaffold for DNA polymerase: pore-size optimisation of mesoporous silica for DNA amplification

RSC Advances ◽  
2014 ◽  
Vol 4 (49) ◽  
pp. 25920-25923 ◽  
Author(s):  
Shun-ichi Matsuura ◽  
Tomoya Baba ◽  
Manami Chiba ◽  
Tatsuo Tsunoda
Keyword(s):  
Mesoporous Silica ◽  
Pore Size ◽  
Dna Polymerase ◽  
Dna Amplification ◽  
Mesoporous Silicas ◽  
Size Regulation ◽  
Thermostable Dna Polymerase

The simple and selective immobilisation of a thermostable DNA polymerase on mesoporous silicas was achieved, and DNA amplification activity was retained under the pore-size regulation of the mesoporous silicas.

scholarly journals Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme

RSC Advances ◽  
2021 ◽  
Vol 11 (17) ◽  
pp. 10010-10017
Author(s):  
Ping-Chung Kuo ◽  
Zhi-Xun Lin ◽  
Tzi-Yi Wu ◽  
Chun-Han Hsu ◽  
Hong-Ping Lin ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Mesoporous Silica ◽  
Pore Size ◽  
Immobilized Enzyme ◽  
Mesoporous Silicas ◽  
High Loading ◽  
Loading Capacity ◽  
Silica Films

Mesoporous silica films were used as supports with high loading capacity and enzyme activity.

scholarly journals Capacity of Nine Thermostable DNA Polymerases To Mediate DNA Amplification in the Presence of PCR-Inhibiting Samples

1998 ◽  
Vol 64 (10) ◽  
pp. 3748-3753 ◽  
Author(s):  
Waleed Abu Al-Soud ◽  
Peter Rådström
Keyword(s):  
Dna Polymerase ◽  
Biological Samples ◽  
Detection Method ◽  
Dna Polymerases ◽  
Dna Amplification ◽  
Nucleic Acid Amplification ◽  
Full Potential ◽  
Thermus Aquaticus ◽  
Pcr Inhibitors ◽  
Thermostable Dna Polymerase

ABSTRACT The PCR is an extremely powerful method for detecting microorganisms. However, its full potential as a rapid detection method is limited by the inhibition of the thermostable DNA polymerase fromThermus aquaticus by many components found in complex biological samples. In this study, we have compared the effects of known PCR-inhibiting samples on nine thermostable DNA polymerases. Samples of blood, cheese, feces, and meat, as well as various ions, were added to PCR mixtures containing various thermostable DNA polymerases. The nucleic acid amplification capacity of the nine polymerases, under buffer conditions recommended by the manufacturers, was evaluated by using a PCR-based detection method for Listeria monocytogenes in the presence of purified template DNA and different concentrations of PCR inhibitors. The AmpliTaqGold and the Taq DNA polymerases from Thermus aquaticus were totally inhibited in the presence of 0.004% (vol/vol) blood in the PCR mixture, while the HotTub, Pwo, rTth, andTfl DNA polymerases were able to amplify DNA in the presence of 20% (vol/vol) blood without reduced amplification sensitivity. The DNA polymerase from Thermotoga maritima(Ultma) was found to be the most susceptible to PCR inhibitors present in cheese, feces, and meat samples. When the inhibitory effect of K and Na ions was tested on the nine polymerases, HotTub from Thermus flavus and rTthfrom Thermus thermophilus were the most resistant. Thus, the PCR-inhibiting effect of various components in biological samples can, to some extent, be eliminated by the use of the appropriate thermostable DNA polymerase.

Swift Synthesis of Hierarchically Ordered Mesocellular Mesoporous Silica by Microwave-Assisted Hydrothermal Method

2008 ◽  
Vol 8 (8) ◽  
pp. 3995-3998 ◽  
Author(s):  
You-Kyong Seo ◽  
I. Suryanarayana ◽  
Young Kyu Hwang ◽  
Namsoo Shin ◽  
Do-Cheon Ahn ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Microwave Irradiation ◽  
Pore Size ◽  
Hydrothermal Method ◽  
Aging Time ◽  
Micropore Volume ◽  
Mesoporous Silicas ◽  
Microwave Assisted ◽  
Silica Source ◽  
Size And Morphology

Hierarchically ordered mesocellular mesoporous silicas (HMMS) were swiftly synthesized using P123 and sodium silicate as a silica source within an hour by applying microwave irradiation without pore expander. The XRD, TEM and BET studies demonstrated that materials as-synthesized and calcined have the hierarchically ordered mesocellular structure with two different sizes about 10 nm and 30 nm pores having a minimized micropore volume compared with conventional hydrothermal method. Moreover, the HMMS sample prepared by microwave-assisted hydrothermal method had higher surface area than that of conventional hydrothermal method. The variation of the pore size and morphology of mesocellular structure varied with the aging time.

scholarly journals A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification

BioTechniques ◽  
2014 ◽  
Vol 57 (2) ◽  
Author(s):  
Konstantin B. Ignatov ◽  
Ekaterina V. Barsova ◽  
Arkady F. Fradkov ◽  
Konstantin A. Blagodatskikh ◽  
Tatiana V. Kramarova ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Dna Amplification ◽  
Strand Displacement ◽  
Displacement Activity ◽  
Thermostable Dna Polymerase

Amplification of RNA: High-Temperature Reverse Transcription and DNA Amplification with a Magnesium-Activated Thermostable DNA Polymerase

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot4115
Author(s):  
Edward S. Smith ◽  
Alvin K. Li ◽  
Alice M. Wang ◽  
David H. Gelfand ◽  
Thomas W. Myers
Keyword(s):  
High Temperature ◽  
Dna Polymerase ◽  
Reverse Transcription ◽  
Dna Amplification ◽  
Thermostable Dna Polymerase

scholarly journals Bifunctional acid–base mesoporous silica@aqueous miscible organic-layered double hydroxides

RSC Advances ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 3749-3754 ◽  
Author(s):  
Hongri Suo ◽  
Haohong Duan ◽  
Chunping Chen ◽  
Jean-Charles Buffet ◽  
Dermot O'Hare
Keyword(s):  
Mesoporous Silica ◽  
Pore Size ◽  
Layered Double Hydroxides ◽  
High Surface ◽  
High Surface Area ◽  
Core Shell ◽  
Acid Base ◽  
Double Hydroxides ◽  
Hierarchical Morphology ◽  
Size And Structure

Core@shell materials which exhibit hierarchical morphology with ultra high surface area and controllable pore size and structure have been synthesised.

Pore Size Engineering of Mesoporous Silicas Using Decane as Expander

Langmuir ◽  
2000 ◽  
Vol 16 (9) ◽  
pp. 4229-4236 ◽  
Author(s):  
J. L. Blin ◽  
C. Otjacques ◽  
G. Herrier ◽  
Bao-Lian Su
Keyword(s):  
Pore Size ◽  
Mesoporous Silicas
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