Functionalized chitosan derived novel positively charged organic–inorganic hybrid ultrafiltration membranes for protein separation

RSC Advances ◽  
2014 ◽  
Vol 4 (42) ◽  
pp. 21699-21711 ◽  
Author(s):  
Mahendra Kumar ◽  
Declan McGlade ◽  
Jenny Lawler

Positively charged organic–inorganic hybrid ultrafiltration membranes for selective protein separation were fabricated from blends of PVA, functionalized600 dpi in TIF format)??> chitosan and tetraethylorthosilicate.

RSC Advances ◽  
2015 ◽  
Vol 5 (63) ◽  
pp. 51208-51219 ◽  
Author(s):  
Mahendra Kumar ◽  
Declan McGlade ◽  
Mathias Ulbricht ◽  
Jenny Lawler

Low fouling positively charged hybrid UF membranes with adjustable charge density fabricated from a blend of PSf/QPSf and GO nanosheets by solution casting and NIPS method. Cross-section SEM image and observed lysozyme transport values at varied pH.


Foods ◽  
2018 ◽  
Vol 7 (10) ◽  
pp. 166 ◽  
Author(s):  
Abhiram Arunkumar ◽  
Mark Etzel

Fractionation of the bovine glycomacropeptide (GMP) from the other proteins in cheese whey was examined using ultrafiltration membranes surface modified to contain positively charged polymer brushes made of polyhexamethylene biguanide. By placing a strong positive charge on a 1000 kDa ultrafiltration membrane and adjusting the pH of whey close to the isoelectric point of GMP, a 14-fold increase in selectivity was observed compared to unmodified membranes. A one stage membrane system gave 90% pure GMP and a three-stage rectification system gave 97% pure GMP. The charged membrane was salt-tolerant up to 40 mS cm−1 conductivity, allowing fractionation of GMP directly from cheese whey without first lowering the whey conductivity by water dilution. Thus, similarly sized proteins that differed somewhat in isoelectric points and were 50–100 fold smaller than the membrane molecular weight cut-off (MWCO), were cleanly fractionated using charged ultrafiltration membranes without water addition. This is the first study to report on the use of salt-tolerant charged ultrafiltration membranes to produce chromatographically pure protein fractions from whey, making ultrafiltration an attractive alternative to chromatography for dairy protein fractionation.


2015 ◽  
Vol 152 ◽  
pp. 55-63 ◽  
Author(s):  
Guan Liu ◽  
Lijuan Zhang ◽  
Shimin Mao ◽  
Sohrab Rohani ◽  
Chibun Ching ◽  
...  

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