scholarly journals Hemisynthesis of deuteriated adenosylhopane and conversion into bacteriohopanetetrol by a cell-free system from Methylobacterium organophilum

2015 ◽  
Vol 13 (11) ◽  
pp. 3393-3405 ◽  
Author(s):  
Wenjun Liu ◽  
Anne Bodlenner ◽  
Michel Rohmer
Keyword(s):  
Reaction Sequence ◽  
Free System ◽  
Cell Free System ◽  
Cross Metathesis ◽  
A Cell

(2H2)Adenosylhopane was synthesised via a reaction sequence including a cross metathesis followed by N22H2 reduction of the resulting olefin.

scholarly journals Metabolism of 4-chloro-2-methylphenoxyacetate by a soil pseudomonad. Ring-fission, lactonizing and delactonizing enzymes

1971 ◽  
Vol 122 (4) ◽  
pp. 533-542 ◽  
Author(s):  
J. K. Gaunt ◽  
W. C. Evans
Keyword(s):  
Metabolic Pathway ◽  
Reduced Glutathione ◽  
Single Step ◽  
Reaction Sequence ◽  
Free System ◽  
Cell Free System ◽  
Ring Fission ◽  
A Cell

1. A cell-free system, prepared from Pseudomonas N.C.I.B. 9340 grown on 4-chloro-2-methylphenoxyacetate (MCPA) was shown to catalyse the reaction sequence: 5-chloro-3-methylcatechol → cis–cis-γ-chloro-α-methylmuconate → γ-carboxymethylene-α-methyl-Δαβ-butenolide → γ-hydroxy-α-methylmuconate. 2. The activity of the three enzymes involved in these reactions was completely resolved and the lactonizing and delactonizing enzymes were separated. 3. This part of the metabolic pathway of 4-chloro-2-methylphenoxyacetate is thus confirmed for this bacterium. 4. The ring-fission oxygenase required Fe2+ or Fe3+ and reduced glutathione for activity; the lactonizing enzyme is stimulated by Mn2+, Mg2+, Co2+ and Fe2+; no cofactor requirement could be demonstrated for the delactonizing enzyme. 5. cis–cis-γ-Chloro-α-methylmuconic acid was isolated and found to be somewhat unstable, readily lactonizing to γ-carboxymethylene-α-methyl-Δαβ-butenolide. 6. Enzymically the lactonization appears to be a single-step dehydrochlorinase reaction.

scholarly journals Effect of detergents on sterol synthesis in a cell-free system of yeast

1982 ◽  
Vol 23 (6) ◽  
pp. 803-810
Author(s):  
S Hata ◽  
T Nishino ◽  
N Ariga ◽  
H Katsuki
Keyword(s):  
Sterol Synthesis ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Reconstitution of Endosomal Proteolysis in a Cell-free System

1989 ◽  
Vol 264 (10) ◽  
pp. 5392-5399
Author(s):  
L S Mayorga ◽  
R Diaz ◽  
P D Stahl
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals A precursor form of latent collagenase produced in a cell-free system with mRNA from rabbit synovial cells.

1981 ◽  
Vol 256 (23) ◽  
pp. 11951-11954 ◽  
Author(s):  
H. Nagase ◽  
R.C. Jackson ◽  
C.E. Brinckerhoff ◽  
C.A. Vater ◽  
E.D. Harris
Keyword(s):  
Synovial Cells ◽  
Free System ◽  
Cell Free System ◽  
A Cell

scholarly journals Analysis of transcriptional stimulation by recombinant Oct proteins in a cell-free system.

1993 ◽  
Vol 268 (4) ◽  
pp. 2525-2534
Author(s):  
A. Annweiler ◽  
S. Zwilling ◽  
R.A. Hipskind ◽  
T. Wirth
Keyword(s):  
Free System ◽  
Cell Free System ◽  
A Cell ◽  
Transcriptional Stimulation
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