A BODIPY-luminol chemiluminescent resonance energy-transfer (CRET) cassette for imaging of cellular superoxide

2015 ◽  
Vol 13 (6) ◽  
pp. 1763-1767 ◽  
Author(s):  
S. Bag ◽  
J.-C. Tseng ◽  
J. Rochford
Keyword(s):  
Reactive Oxygen Species ◽  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Reactive Oxygen ◽  
Resonance Energy ◽  
Oxygen Species

Chemiluminescent resonance energy transfer is investigated for a BODIPY-luminol dyad demonstratingin cellulobiochemiluminescence with reactive oxygen species in activated splenocytes.

THE ROLES OF PHOTOLUMINESCENT QUANTUM DOTS IN GENERATION OR DETECTION OF REACTIVE OXYGEN SPECIES: CULPRITS OR DETECTIVES?

COSMOS ◽  
2010 ◽  
Vol 06 (02) ◽  
pp. 149-158
Author(s):  
SUHUA WANG ◽  
DEJIAN HUANG
Keyword(s):  
Reactive Oxygen Species ◽  
Quantum Dots ◽  
Organic Dyes ◽  
Resonance Energy Transfer ◽  
Reactive Oxygen ◽  
Resonance Energy ◽  
Oxygen Species ◽  
Positive Side ◽  
Biological Importance ◽  
Organic Sensitizers

In this review, we systematically analyzed the complicated interrelationship between photoluminescent quantum dots (QDs) and reactive oxygen species of biological importance. QDs, when photoexcited, generate reactive oxygen species (ROS), which are partially blamed for the cytotoxicity of QDs. On the positive side, the ability of generating ROS by QDs are exploited in photodynamic therapy using QDs alone or in combination with QD-surface bound organic sensitizers via resonance energy transfer from QDs to the organic dyes. Lastly, depending on the chemical composition and the functionalization of the QDs, ROS are known to quench or switch-on the QD photoluminescence. The selectivity and sensitivity toward specific ROS can be achieved through judicious chemical modification of QD surface coating layers by taking into account the reactivity difference among different ROS. The flexible QD surface functionalization opens up the unprecedented possibility of designer-made nanoprobes for sensing and quantifying ROS of biological importance.

scholarly journals Luciferase–Rose Bengal conjugates for singlet oxygen generation by bioluminescence resonance energy transfer

2017 ◽  
Vol 53 (33) ◽  
pp. 4569-4572 ◽  
Author(s):  
Seonghoon Kim ◽  
HyeongChan Jo ◽  
Mijeong Jeon ◽  
Myung-Gyu Choi ◽  
Sei Kwang Hahn ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Energy Transfer ◽  
Singlet Oxygen ◽  
Rose Bengal ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Oxygen Species ◽  
Bioluminescence Resonance Energy Transfer ◽  
Oxygen Generation ◽  
Singlet Oxygen Generation

Luciferase–Rose Bengal conjugates generated reactive oxygen species (ROS) inside cells via bioluminescence resonance energy transfer (BRET) without external light irradiation.

scholarly journals Synthesis and optimization of a reactive oxygen species responsive cellular delivery system

2017 ◽  
Vol 41 (6) ◽  
pp. 2392-2400 ◽  
Author(s):  
Ana M. Perez-Lopez ◽  
Elsa Valero ◽  
Mark Bradley
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Near Infrared ◽  
Resonance Energy Transfer ◽  
Reactive Oxygen ◽  
Resonance Energy ◽  
Oxygen Species ◽  
Cellular Delivery ◽  
System P ◽  
Self Immolation

Reactive oxygen species responsive delivery systems for the detection of peroxides in live macrophages have been designed. The oxidative cleavage of a boronic ester to a phenol triggered by hydrogen peroxide followed by self-immolation of a ROS-sensitive cleavable linkervia1,6-elimination allowed the disturbance of the fluorescence resonance energy transfer turning on the near-infrared fluorescence.

scholarly journals Light-harvesting mutants show differential gene expression upon shift to high light as a consequence of photosynthetic redox and reactive oxygen species metabolism

2014 ◽  
Vol 369 (1640) ◽  
pp. 20130229 ◽  
Author(s):  
Mikko Tikkanen ◽  
Peter J. Gollan ◽  
Nageswara Rao Mekala ◽  
Janne Isojärvi ◽  
Eva-Mari Aro
Keyword(s):  
Gene Expression ◽  
Reactive Oxygen Species ◽  
Energy Transfer ◽  
Excitation Energy ◽  
Light Harvesting ◽  
Expression Profiles ◽  
Excitation Energy Transfer ◽  
Reactive Oxygen ◽  
High Light ◽  
Oxygen Species

The amount of light energy that is harvested and directed to the photosynthetic machinery is regulated in order to control the production of reactive oxygen species (ROS) in leaf tissues. ROS have important roles as signalling factors that instigate and mediate a range of cellular responses, suggesting that the mechanisms regulating light-harvesting and photosynthetic energy transduction also affect cell signalling. In this study, we exposed wild-type (WT) Arabidopsis and mutants impaired in the regulation of photosynthetic light-harvesting ( stn7 , tap38 and npq4 ) to transient high light (HL) stress in order to study the role of these mechanisms for up- and downregulation of gene expression under HL stress. The mutants, all of which have disturbed regulation of excitation energy transfer and distribution, responded to transient HL treatment with surprising similarity to the WT in terms of general ‘abiotic stress-regulated’ genes associated with hydrogen peroxide and 12-oxo-phytodienoic acid signalling. However, we identified distinct expression profiles in each genotype with respect to induction of singlet oxygen and jasmonic acid-dependent responses. The results of this study suggest that the control of excitation energy transfer interacts with hormonal regulation. Furthermore, the photosynthetic pigment–protein complexes appear to operate as receptors that sense the energetic balance between the photosynthetic light reactions and downstream metabolism.

Mitochondria as a source of reactive oxygen species

2009 ◽  
pp. c3 ◽  
Author(s):  
Helena M. Cochemé ◽  
Michael P. Murphy
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species

Clinical aspects of reactive oxygen and nitrogen species

2004 ◽  
Vol 71 ◽  
pp. 121-133 ◽  
Author(s):  
Ascan Warnholtz ◽  
Maria Wendt ◽  
Michael August ◽  
Thomas Münzel
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Risk Factor ◽  
Endothelial Dysfunction ◽  
Vascular Tissue ◽  
Rapid Development ◽  
Reactive Oxygen ◽  
Clinical Aspects ◽  
No Production ◽  
Oxygen Species

Endothelial dysfunction in the setting of cardiovascular risk factors, such as hypercholesterolaemia, hypertension, diabetes mellitus and chronic smoking, as well as in the setting of heart failure, has been shown to be at least partly dependent on the production of reactive oxygen species in endothelial and/or smooth muscle cells and the adventitia, and the subsequent decrease in vascular bioavailability of NO. Superoxide-producing enzymes involved in increased oxidative stress within vascular tissue include NAD(P)H-oxidase, xanthine oxidase and endothelial nitric oxide synthase in an uncoupled state. Recent studies indicate that endothelial dysfunction of peripheral and coronary resistance and conductance vessels represents a strong and independent risk factor for future cardiovascular events. Ways to reduce endothelial dysfunction include risk-factor modification and treatment with substances that have been shown to reduce oxidative stress and, simultaneously, to stimulate endothelial NO production, such as inhibitors of angiotensin-converting enzyme or the statins. In contrast, in conditions where increased production of reactive oxygen species, such as superoxide, in vascular tissue is established, treatment with NO, e.g. via administration of nitroglycerin, results in a rapid development of endothelial dysfunction, which may worsen the prognosis in patients with established coronary artery disease.

Fas ligand expression in rat kupffer cells in response to lipopolysaccharide is mediated by reactive oxygen species

2001 ◽  
Vol 120 (5) ◽  
pp. A361-A361
Author(s):  
K UCHIKURA ◽  
T WADA ◽  
Z SUN ◽  
S HOSHINO ◽  
G BULKLEY ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Kupffer Cells ◽  
Fas Ligand ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Ligand Expression
Sign in / Sign up

Export Citation Format

Share Document