Synthesis and photophysical characterisation of new fluorescent triazole adenine analogues

Christopher P. Lawson; Anke Dierckx; Francois-Alexandre Miannay; Eric Wellner; L. Marcus Wilhelmsson; Morten Grøtli

doi:10.1039/c4ob00904e

Synthesis and photophysical characterisation of new fluorescent triazole adenine analogues

Organic & Biomolecular Chemistry ◽

10.1039/c4ob00904e ◽

2014 ◽

Vol 12 (28) ◽

pp. 5158-5167 ◽

Cited By ~ 13

Author(s):

Christopher P. Lawson ◽

Anke Dierckx ◽

Francois-Alexandre Miannay ◽

Eric Wellner ◽

L. Marcus Wilhelmsson ◽

...

Keyword(s):

Physical Properties ◽

Adenine Analogues ◽

Adenine Derivatives

Novel fluorescent triazole adenine derivatives have been synthesised and their photo-physical properties characterised.

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APRT SELECTION SYSTEM IN PLANTS

HortScience ◽

10.21273/hortsci.27.11.1160f ◽

1992 ◽

Vol 27 (11) ◽

pp. 1160f-1160

Author(s):

Sunita K. Agarwal ◽

David J. Schultz ◽

Dennis A. Schaff

Keyword(s):

De Novo ◽

Selection System ◽

Wild Type ◽

Purine Salvage ◽

Toxic Plants ◽

Adenine Analogues ◽

Salvage Pathways ◽

Cloned Gene ◽

High Degree ◽

Adenine Derivatives

Most cells have an active turnover of many of their nucleic acids (particularly some types of RNA) which through degradative processes result in the release of adenine, guanine and hypoxanthine. These free purines are converted to their corresponding nucleotides through salvage pathways. Adenine is converted to its nucleotide form AMP by Adenine phosphoribosyltransferase (APRT) which is one of the enzymes associated with the purine salvage pathway. Since all organisms have a de novo pathway for the formation of AMP, APRT is classified as a `salvage enzyme'. The APRT enzyme, in general, does not show a high degree of specificity for the exact structure of adenine and can also act on cytokinins and adenine derivatives like 2,6-diaminopurine, 2-fluoroadenine and 6-methylpurine. The APRT enzyme can utilize adenine analogues as substrate and convert them into their nucleotide forms which are toxic. Plants that lack APRT activity (APRT-plants) survive in the presense of these analogues. The amount of adenine analogue used for selecting APRT-plants is such that it kills all APRT+ (wild type) plants. APRT+ plants survive when grown in the presense of azaserine and alanosine that block de novo synthesis of AMP. APRT-plants transformed with the wild type cloned gene can be selected from a mixture of transformed and non-transformed plants by selecting in the presense of adenine, azaserine and alanosine. The presense of APRT activity can be confirmed by assaying for the APRT enzyme. APRT activity has been detected in many plant species. The presense of a positive forward and backward selection system can thus allow the use of APRT as a selectable marker in plant gene transfer systems.

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The Photometric Properties of the Ap Stars

International Astronomical Union Colloquium ◽

10.1017/s0252921100080684 ◽

1976 ◽

Vol 32 ◽

pp. 365-377 ◽

Cited By ~ 2

Author(s):

B. Hauck

Keyword(s):

Physical Properties ◽

Ap Stars

The Ap stars are numerous - the photometric systems tool It would be very tedious to review in detail all that which is in the literature concerning the photometry of the Ap stars. In my opinion it is necessary to examine the problem of the photometric properties of the Ap stars by considering first of all the possibility of deriving some physical properties for the Ap stars, or of detecting new ones. My talk today is prepared in this spirit. The classification by means of photoelectric photometric systems is at the present time very well established for many systems, such as UBV, uvbyβ, Vilnius, Geneva and DDO systems. Details and methods of classification can be found in Golay (1974) or in the proceedings of the Albany Colloquium edited by Philip and Hayes (1975).

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The Infection of Cells by Bullet-Shaped Viruses

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100063779 ◽

1969 ◽

Vol 27 ◽

pp. 372-373

Author(s):

Frederick A. Murphy ◽

Alyne K. Harrison ◽

Sylvia G. Whitfield

Keyword(s):

Electron Microscopy ◽

Physical Properties ◽

Host Cell ◽

Thin Section ◽

Cultured Cells ◽

Cell Infection ◽

Thin Section Electron Microscopy ◽

Section Electron ◽

Section Electron Microscopy

The bullet-shaped viruses are currently classified together on the basis of similarities in virion morphology and physical properties. Biologically and ecologically the member viruses are extremely diverse. In searching for further bases for making comparisons of these agents, the nature of host cell infection, both in vivo and in cultured cells, has been explored by thin-section electron microscopy.

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Transmission electron microscopy of composite materials

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100107125 ◽

1988 ◽

Vol 46 ◽

pp. 1012-1015

Author(s):

K.P.D. Lagerlof

Keyword(s):

Composite Materials ◽

Physical Properties ◽

Structural Defects ◽

Structural Composites ◽

Multiphase Materials ◽

Structural Reinforcement ◽

Transmission Electron ◽

Reinforced Fiber ◽

Particulate Reinforced Composites ◽

Definition Of

Although most materials contain more than one phase, and thus are multiphase materials, the definition of composite materials is commonly used to describe those materials containing more than one phase deliberately added to obtain certain desired physical properties. Composite materials are often classified according to their application, i.e. structural composites and electronic composites, but may also be classified according to the type of compounds making up the composite, i.e. metal/ceramic, ceramic/ceramie and metal/semiconductor composites. For structural composites it is also common to refer to the type of structural reinforcement; whisker-reinforced, fiber-reinforced, or particulate reinforced composites [1-4].For all types of composite materials, it is of fundamental importance to understand the relationship between the microstructure and the observed physical properties, and it is therefore vital to properly characterize the microstructure. The interfaces separating the different phases comprising the composite are of particular interest to understand. In structural composites the interface is often the weakest part, where fracture will nucleate, and in electronic composites structural defects at or near the interface will affect the critical electronic properties.

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