Marine bacteria from the Roseobacter clade produce sulfur volatiles via amino acid and dimethylsulfoniopropionate catabolism

2014 ◽  
Vol 12 (25) ◽  
pp. 4318 ◽  
Author(s):  
Nelson L. Brock ◽  
Markus Menke ◽  
Tim A. Klapschinski ◽  
Jeroen S. Dickschat
Keyword(s):  
Amino Acid ◽  
Marine Bacteria ◽  
Roseobacter Clade ◽  
Sulfur Volatiles

D-alanine metabolism via D-Ala aminotransferase by marine Gammaproteobacteria , Pseudoalteromonas sp. CF6-2

2021 ◽  
Author(s):  
Yang Yu ◽  
Jie Yang ◽  
Zhao-Jie Teng ◽  
Li-Yuan Zheng ◽  
Qi Sheng ◽  
...  
Keyword(s):  
Amino Acid ◽  
Marine Bacteria ◽  
Gene Knockout ◽  
Underlying Mechanism ◽  
Dominant Group ◽  
Seawater Samples ◽  
Underlying Mechanisms ◽  
Biochemical Analyses ◽  
Global Oceans ◽  
Shed Light

As the most abundant D-amino acid (DAA) in the ocean, D-alanine (D-Ala) is a key component of peptidoglycan in bacterial cell wall. However, the underlying mechanisms of bacterial metabolization of D-Ala through microbial food web remain largely unknown. In this study, the metabolism of D-Ala by marine bacterium Pseudoalteromonas sp. CF6-2 was investigated. Based on genomic, transcriptional and biochemical analyses combined with gene knockout, D-Ala aminotransferase was found to be indispensable for the catabolism of D-Ala in strain CF6-2. Investigation on other marine bacteria also showed that D-Ala aminotransferase gene is a reliable indicator for their ability to utilize D-Ala. Bioinformatic investigation revealed that D-Ala aminotransferase sequences are prevalent in genomes of marine bacteria and metagenomes, especially in seawater samples, and Gammaproteobacteria represents the predominant group containing D-Ala aminotransferase. Thus, Gammaproteobacteria is likely the dominant group to utilize D-Ala via D-Ala aminotransferase to drive the recycling and mineralization of D-Ala in the ocean. IMPORTANCE As the most abundant D-amino acid in the ocean, D-Ala is a component of marine DON (Dissolved organic nitrogen) pool. However, the underlying mechanism of bacterial metabolization of D-Ala to drive the recycling and mineralization of D-Ala in the ocean is still largely unknown. The results in this study showed that D-Ala aminotransferase is specific and indispensable for D-Ala catabolism in marine bacteria, and that marine bacteria containing D-Ala aminotransferase genes are predominantly Gammaproteobacteria widely distributed in global oceans. This study reveals marine D-Ala utilizing bacteria and the mechanism of their metabolization of D-Ala. The results shed light on the mechanisms of recycling and mineralization of D-Ala driven by bacteria in the ocean, which are helpful in understanding oceanic microbial-mediated nitrogen cycle.

Detection of the Na+-translocating NADH-quinone reductase in marine bacteria using a PCR technique

2000 ◽  
Vol 46 (4) ◽  
pp. 325-332 ◽  
Author(s):  
Sanae Kato ◽  
Isao Yumoto
Keyword(s):  
Phylogenetic Analysis ◽  
Amino Acid ◽  
16S Rrna ◽  
Marine Bacteria ◽  
Genomic Dna ◽  
Screening Method ◽  
Quinone Reductase ◽  
Amino Acid Sequences ◽  
Homologous Sequences ◽  
Simple Screening

To examine the distribution of the Na+-translocating NADH-quinone reductase (Na+-NQR) among marine bacteria, we developed a simple screening method for the detection of this enzyme. By reference to the homologous sequences of the Na+-NQR operons from Vibrio alginolyticus and Haemophilus influenzae, a pair of primers was designed for amplification of a part of the sixth ORF (nqr6) of the Na+-NQR operon. When PCR was performed using genomic DNA from 13 marine bacteria, a 0.9-kbp fragment corresponding to nqr6 was amplified in 10 strains. Although there were three PCR-negative strains phylogenetically, based on the sequence of the 16S rRNA, these were placed far from the PCR-positive strains. No product was observed in the case of nonmarine bacteria. The nucleotide and predicted amino acid sequences of nqr6 were highly conserved among the PCR-positive marine bacteria. A phylogenetic analysis of marine bacteria, based on nqr6 sequencing, was performed.Key words: Na+-translocating, NADH-quinone reductase, marine bacteria, PCR.

scholarly journals Antibiotic Production by a Roseobacter Clade-Affiliated Species from the German Wadden Sea and Its Antagonistic Effects on Indigenous Isolates

2004 ◽  
Vol 70 (4) ◽  
pp. 2560-2565 ◽  
Author(s):  
Thorsten Brinkhoff ◽  
Gabriela Bach ◽  
Thorsten Heidorn ◽  
Lanfang Liang ◽  
Andrea Schlingloff ◽  
...  
Keyword(s):  
Marine Bacteria ◽  
Growth Phase ◽  
Marine Algae ◽  
Wadden Sea ◽  
Antibiotic Production ◽  
Roseobacter Clade ◽  
Antagonistic Effects ◽  
Tropodithietic Acid

ABSTRACT A strain affiliated with the Roseobacter clade and producing a new antibiotic named tropodithietic acid (L. Liang, Ph.D. thesis, University of Göttingen, Göttingen, Germany, 2003) was isolated from the German Wadden Sea. The compound showed strong inhibiting properties with respect to marine bacteria of various taxa and marine algae. Antibiotic production was found to occur during the complete growth phase. Strain mutants without antagonistic properties appeared several times spontaneously.

scholarly journals Breakdown of 3-(allylsulfonio)propanoates in bacteria from the Roseobacter group yields garlic oil constituents

2021 ◽  
Vol 17 ◽  
pp. 569-580
Author(s):  
Anuj Kumar Chhalodia ◽  
Jeroen S Dickschat
Keyword(s):  
Marine Bacteria ◽  
Dimethyl Sulfide ◽  
Mass Spectrometric ◽  
Fragmentation Pattern ◽  
Garlic Oil ◽  
Feeding Experiments ◽  
Sulfur Volatiles ◽  
Roseobacter Group ◽  
Mass Spectrometric Fragmentation ◽  
Substrate Tolerance

Two analogues of 3-(dimethylsulfonio)propanoate (DMSP), 3-(diallylsulfonio)propanoate (DAllSP), and 3-(allylmethylsulfonio)propanoate (AllMSP), were synthesized and fed to marine bacteria from the Roseobacter clade. These bacteria are able to degrade DMSP into dimethyl sulfide and methanethiol. The DMSP analogues were also degraded, resulting in the release of allylated sulfur volatiles known from garlic. For unknown compounds, structural suggestions were made based on their mass spectrometric fragmentation pattern and confirmed by the synthesis of reference compounds. The results of the feeding experiments allowed to conclude on the substrate tolerance of DMSP degrading enzymes in marine bacteria.

Kinetics of glucose and amino acid uptake by attached and free-living marine bacteria in oligotrophic waters

2001 ◽  
Vol 138 (5) ◽  
pp. 1071-1076 ◽  
Author(s):  
B. Ayo ◽  
M. Unanue ◽  
I. Azúa ◽  
G. Gorsky ◽  
C. Turley ◽  
...  
Keyword(s):  
Amino Acid ◽  
Marine Bacteria ◽  
Amino Acid Uptake ◽  
Acid Uptake ◽  
Free Living ◽  
Kinetics Of

scholarly journals Distribution in Different Organisms of Amino Acid Oxidases with FAD or a Quinone As Cofactor and Their Role as Antimicrobial Proteins in Marine Bacteria

Marine Drugs ◽  
2015 ◽  
Vol 13 (12) ◽  
pp. 7403-7418 ◽  
Author(s):  
Jonatan Campillo-Brocal ◽  
Patricia Lucas-Elío ◽  
Antonio Sanchez-Amat
Keyword(s):  
Amino Acid ◽  
Marine Bacteria ◽  
Antimicrobial Proteins

scholarly journals Development and Application of Quantitative-PCR Tools for Subgroups of the Roseobacter Clade

2009 ◽  
Vol 75 (23) ◽  
pp. 7542-7547 ◽  
Author(s):  
Alison Buchan ◽  
Mary Hadden ◽  
Marcelino T. Suzuki
Keyword(s):  
Quantitative Pcr ◽  
Marine Bacteria ◽  
Internal Transcribed Spacer ◽  
Sybr Green ◽  
23S Rrna ◽  
Roseobacter Clade ◽  
Internal Transcribed Spacer Regions ◽  
Conserved Regions ◽  
Field Samples ◽  
Pcr Assays

ABSTRACT Specific SYBR green-based quantitative-PCR assays targeting conserved regions in the 16S-23S rRNA internal transcribed spacer regions were developed for five subgroups of the environmentally abundant and biogeochemically active Roseobacter clade of marine bacteria. The assays were applied to field samples demonstrating their utility in investigations of abundant Roseobacter group phylotypes in the environment.

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