scholarly journals Annexin-V/quantum dot probes for multimodal apoptosis monitoring in living cells: improving bioanalysis using electrochemistry

Nanoscale ◽  
2015 ◽  
Vol 7 (9) ◽  
pp. 4097-4104 ◽  
Author(s):  
Helena Montón ◽  
Claudio Parolo ◽  
Antonio Aranda-Ramos ◽  
Arben Merkoçi ◽  
Carme Nogués
Keyword(s):  
Quantum Dot ◽  
Electrochemical Detection ◽  
Cell Cultures ◽  
Annexin V ◽  
Cost Effective ◽  
Living Cells ◽  
Qualitative And Quantitative ◽  
Apoptosis Detection ◽  
Quantitative Results

A novel-electrochemical Annexin-V–QD conjugate, which can be also used as a classical-optical label, is applied for apoptosis detection in THP-1 cell cultures. The electrochemical detection of AnnV–QD labeled cells gives qualitative and quantitative results in a fast and cost-effective way.

scholarly journals Graphene, an Interesting Nanocarbon Allotrope for Biosensing Applications: Advances, Insights, and Prospects

2021 ◽  
Vol 12 ◽  
pp. 117959722098382
Author(s):  
Farid Menaa ◽  
Yazdian Fatemeh ◽  
Sandeep K Vashist ◽  
Haroon Iqbal ◽  
Olga N Sharts ◽  
...  
Keyword(s):  
Cost Effective ◽  
Genetic Alterations ◽  
Multiplex Detection ◽  
Quantitative Characterization ◽  
Disease Biomarkers ◽  
Qualitative And Quantitative ◽  
Sensing Systems ◽  
Doped Graphene ◽  
Bioanalytical Applications

Graphene, a relatively new two-dimensional (2D) nanomaterial, possesses unique structure (e.g. lighter, harder, and more flexible than steel) and tunable physicochemical (e.g. electronical, optical) properties with potentially wide eco-friendly and cost-effective usage in biosensing. Furthermore, graphene-related nanomaterials (e.g. graphene oxide, doped graphene, carbon nanotubes) have inculcated tremendous interest among scientists and industrials for the development of innovative biosensing platforms, such as arrays, sequencers and other nanooptical/biophotonic sensing systems (e.g. FET, FRET, CRET, GERS). Indeed, combinatorial functionalization approaches are constantly improving the overall properties of graphene, such as its sensitivity, stability, specificity, selectivity, and response for potential bioanalytical applications. These include real-time multiplex detection, tracking, qualitative, and quantitative characterization of molecules (i.e. analytes [H2O2, urea, nitrite, ATP or NADH]; ions [Hg2+, Pb2+, or Cu2+]; biomolecules (DNA, iRNA, peptides, proteins, vitamins or glucose; disease biomarkers such as genetic alterations in BRCA1, p53) and cells (cancer cells, stem cells, bacteria, or viruses). However, there is still a paucity of comparative reports that critically evaluate the relative toxicity of carbon nanoallotropes in humans. This manuscript comprehensively reviews the biosensing applications of graphene and its derivatives (i.e. GO and rGO). Prospects and challenges are also introduced.

scholarly journals Marine Fish Primary Hepatocyte Isolation and Culture: New Insights to Enzymatic Dissociation Pancreatin Digestion

2021 ◽  
Vol 18 (4) ◽  
pp. 1380
Author(s):  
Neusa Figueiredo ◽  
Beatriz Matos ◽  
Mário Diniz ◽  
Vasco Branco ◽  
Marta Martins
Keyword(s):  
Cell Viability ◽  
Cell Cultures ◽  
Marine Fish ◽  
Sparus Aurata ◽  
Cost Effective ◽  
Primary Hepatocyte ◽  
Digestion Method ◽  
Trypan Blue Exclusion ◽  
And Function

Primary cell cultures from wild organisms have been gaining relevance in ecotoxicology as they are considered more sensitive than immortalized cell lines and retain the biochemical pathways found in vivo. In this study, the efficacy of two methods for primary hepatocyte cell isolation was compared using liver from two marine fish (Sparus aurata and Psetta maxima): (i) two-step collagenase perfusion and (ii) pancreatin digestion with modifications. Cell cultures were incubated in L-15 medium at 17 ± 1 °C and monitored for up to six days for cell viability and function using the trypan blue exclusion test, MTT test, lactate dehydrogenase (LDH) activity, and ethoxyresorufin O-deethylase (EROD) activity after Benzo[a]Pyrene exposure. The results showed significant differences between the number of viable cells (p < 0.05), the highest number being obtained for the pancreatin digestion method (average = 4.5 ± 1.9 × 107 cells). Moreover, the hepatocytes showed solid adherence to the culture plate and the rounded shape, changing into a triangular/polygonal shape. The cell viability and function obtained by pancreatin digestion were maintained for five days, and the EROD induction after exposure to the B[a]P showed that cells were metabolically active. This study shows that the optimized pancreatin digestion method is a valid, cost-effective, and simple alternative to the standard perfusion method for the isolation of primary hepatocytes from fish and is suitable for ecotoxicological studies involving marine pollutants, such as PAHs.

scholarly journals Affective communication: a mixed method investigation into COVID-19 outbreak communication using the Taiwanese government Facebook page

2021 ◽  
pp. 175797592110035
Author(s):  
Chia Yu Lien ◽  
Yun-Hsuan Wu
Keyword(s):  
Social Media ◽  
Mixed Method ◽  
Health Authority ◽  
Disease Outbreak ◽  
Government Agencies ◽  
Affective Communication ◽  
Frontline Workers ◽  
The Public ◽  
Qualitative And Quantitative ◽  
Quantitative Results

The COVID-19 outbreak has created an unprecedented challenge for governments to convey information to the public, and social media has become a critical method of COVID-19 communication in Taiwan. Objectives: This study examines a total of 1128 Facebook posts published by Taiwan’s principal health authority from December 1, 2019 to May 31, 2020. Methods: Using both qualitative and quantitative approaches, this study investigates strategies used by the Taiwan government to communicate the COVID-19 outbreak and public responses toward these strategies. Result: Novel uses of Facebook posts on outbreak communication were identified, including solidarity, reviews of actions, press conferences, and the use of animal and cartoon images. Quantitative results showed that the public responded significantly more frequently to messages generating positive affects, such as posts that reviewed government actions and public efforts; posts that expressed thanks, approval, or comradeship; and posts that paired text with photographs of frontline workers or cute animals. Conclusion: These results suggest that, amid a disease outbreak, the public not only look for updated situations and guidelines but also for affective affirmation from government agencies.

scholarly journals Quantum Dot Sensors and Nanoactuators in Living Cells

2010 ◽  
Vol 98 (3) ◽  
pp. 394a
Author(s):  
Guillermo Menendez ◽  
M. Julia Roberti ◽  
Maria H. Etchehon ◽  
Thomas M. Jovin ◽  
Elizabeth A. Jares-Erijman
Keyword(s):  
Quantum Dot ◽  
Living Cells

Multidirection Validation of a Finite Element 50th Percentile Male Hybrid III Anthropomorphic Test Device for Spaceflight Applications

2019 ◽  
Vol 141 (3) ◽  
Author(s):  
Derek A. Jones ◽  
James P. Gaewsky ◽  
Mona Saffarzadeh ◽  
Jacob B. Putnam ◽  
Ashley A. Weaver ◽  
...  
Keyword(s):  
Finite Element ◽  
Injury Risk ◽  
Fe Model ◽  
Fe Modeling ◽  
Test Device ◽  
Qualitative And Quantitative ◽  
Hybrid Iii ◽  
Physical Tests ◽  
Quantitative Results ◽  
Male Hybrid

The use of anthropomorphic test devices (ATDs) for calculating injury risk of occupants in spaceflight scenarios is crucial for ensuring the safety of crewmembers. Finite element (FE) modeling of ATDs reduces cost and time in the design process. The objective of this study was to validate a Hybrid III ATD FE model using a multidirection test matrix for future spaceflight configurations. Twenty-five Hybrid III physical tests were simulated using a 50th percentile male Hybrid III FE model. The sled acceleration pulses were approximately half-sine shaped, and can be described as a combination of peak acceleration and time to reach peak (rise time). The range of peak accelerations was 10–20 G, and the rise times were 30–110 ms. Test directions were frontal (−GX), rear (GX), vertical (GZ), and lateral (GY). Simulation responses were compared to physical tests using the correlation and analysis (CORA) method. Correlations were very good to excellent and the order of best average response by direction was −GX (0.916±0.054), GZ (0.841±0.117), GX (0.792±0.145), and finally GY (0.775±0.078). Qualitative and quantitative results demonstrated the model replicated the physical ATD well and can be used for future spaceflight configuration modeling and simulation.

Nonlinear Analysis of a Rigid Rotor on Magnetic Bearings

1995 ◽  
Author(s):  
C. Nataraj
Keyword(s):  
Nonlinear Analysis ◽  
Equations Of Motion ◽  
Forced Response ◽  
Magnetic Bearings ◽  
Rigid Rotor ◽  
Stability Criteria ◽  
Safe Operation ◽  
Qualitative And Quantitative ◽  
Quantitative Results ◽  
Nonlinear Equations Of Motion

A simple model of a rigid rotor supported on magnetic bearings is considered. A proportional control architecture is assumed, the nonlinear equations of motion are derived and some essential nondimensional parameters are identified. The free and forced response of the system is analyzed using techniques of nonlinear analysis. Both qualitative and quantitative results are obtained and stability criteria are derived for safe operation of the system.

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