scholarly journals Global transcriptomic analysis of model human cell lines exposed to surface-modified gold nanoparticles: the effect of surface chemistry

Nanoscale ◽  
2015 ◽  
Vol 7 (4) ◽  
pp. 1349-1362 ◽  
Author(s):  
E. M. Grzincic ◽  
J. A. Yang ◽  
J. Drnevich ◽  
P. Falagan-Lotsch ◽  
C. J. Murphy
Keyword(s):  
Gold Nanoparticles ◽  
Surface Chemistry ◽  
Cell Lines ◽  
Human Cell ◽  
Transcriptomic Analysis ◽  
Surface Coatings ◽  
Human Cell Lines ◽  
Surface Modified ◽  
20 Nm ◽  
Effect Of Surface

Global transcriptomic analysis of human cell lines exposed to 20 nm gold nanoparticles of different, but related surface coatings show how aspects of surface chemistry can alter gene expression.

scholarly journals Enhanced Cellular Uptake of H-Chain Human Ferritin Containing Gold Nanoparticles

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1966
Author(s):  
Italo Moglia ◽  
Margarita Santiago ◽  
Simon Guerrero ◽  
Mónica Soler ◽  
Alvaro Olivera-Nappa ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Lines ◽  
Cellular Uptake ◽  
Human Cell ◽  
Animal Experiments ◽  
Cell Types ◽  
Biological Properties ◽  
Human Cell Lines ◽  
Theranostic Agents

Gold nanoparticles (AuNP) capped with biocompatible layers have functional optical, chemical, and biological properties as theranostic agents in biomedicine. The ferritin protein containing in situ synthesized AuNPs has been successfully used as an effective and completely biocompatible nanocarrier for AuNPs in human cell lines and animal experiments in vivo. Ferritin can be uptaken by different cell types through receptor-mediated endocytosis. Despite these advantages, few efforts have been made to evaluate the toxicity and cellular internalization of AuNP-containing ferritin nanocages. In this work, we study the potential of human heavy-chain (H) and light-chain (L) ferritin homopolymers as nanoreactors to synthesize AuNPs and their cytotoxicity and cellular uptake in different cell lines. The results show very low toxicity of ferritin-encapsulated AuNPs on different human cell lines and demonstrate that efficient cellular ferritin uptake depends on the specific H or L protein chains forming the ferritin protein cage and the presence or absence of metallic cargo. Cargo-devoid apoferritin is poorly internalized in all cell lines, and the highest ferritin uptake was achieved with AuNP-loaded H-ferritin homopolymers in transferrin-receptor-rich cell lines, showing more than seven times more uptake than apoferritin.

scholarly journals CYTOTOXICITY EVALUATION OF TITANIUM AND ZINC OXIDE NANOPARTICLES ON HUMAN CELL LINES

2017 ◽  
Vol 9 (10) ◽  
pp. 240
Author(s):  
Durgaiah Gandamalla ◽  
Harikiran Lingabathula ◽  
Narsimha Reddy Yellu
Keyword(s):  
Zinc Oxide ◽  
Cell Viability ◽  
Human Skin ◽  
Cell Lines ◽  
Human Cell ◽  
Cell Damage ◽  
Human Cell Lines ◽  
Hep G2 ◽  
20 Nm

Objective: In vitro cytotoxicity evaluation of titanium dioxide, 20 nm (TNP 20) and zinc oxide, 20 nm (ZNP 20) nanoparticles (NP) were tested on different types of human skin (HaCat), lung (A549), liver (Hep G2) and colon (Caco-2) cell cultures in relevance to human risk assessmentMethods: The different concentrations of test TNP 20 and ZNP 20 1-300 µg/ml were exposed to determine the cell viability reduction on four human cell lines after 48 h post exposure using 3-(4, 5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The mitochondrial membrane activities of the viable cells were determined with intensity of formazon formation by interpreting ELISA absorbance values at 470 nm.Results: The percent of cytotoxicity was determined by comparing percentage of cell viability reduction of test with that of control. The ZNP 20 produced higher cytotoxicity at the doses 100 (p<0.05) and 300 (p<0.001) µg/ml significantly on tested four human skin (HaCaT), lung (A549), liver (Hep G2) and colon (Caco-2) cells compared to TNP 20. The tested NP induced lesser cytotoxicity at lower concentrations with 1 and 3µg/ml in all the tested four cell lines. The induced cytotoxicity was an indicator for increased intracellular reactive oxygen species which further cause’s major cell damage and cell death.Conclusion: The tested NP were induced greater cytotoxicity in the colon, Liver, lung and skin cells at higher concentrations 100 and 300 µg/ml significantly. The cytotoxicity order of TNP 20 and ZNP 20 at the highest dose (300µg/ml) were concluded as Caco-2>Hep G2>A549>HaCaT for 48 h post exposed cells.

Investigations of the influence of microgravity on the state of human cell lines

2004 ◽  
Vol 10 (5-6) ◽  
pp. 226-228
Author(s):  
L.M. Nosach ◽  
◽  
O.Yu. Povnitsa ◽  
V.L. Zhovnovata ◽  
◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
The State ◽  
Human Cell Lines

scholarly journals Dicalcin suppresses in vitro trophoblast attachment in human cell lines

2021 ◽  
Vol 570 ◽  
pp. 206-213
Author(s):  
Ryohei Saito ◽  
Hiromasa Satoh ◽  
Kayo Aoba ◽  
Hajime Hirasawa ◽  
Naofumi Miwa
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines

scholarly journals Differential Effects of Cyanidin and Cyanidin-3-glucoside on Human Cell Lines

2011 ◽  
Vol 17 (6) ◽  
pp. 515-521 ◽  
Author(s):  
Masayuki TAKEUCHI ◽  
Katsuki OHTANI ◽  
Yanju MA ◽  
Sanae KATO ◽  
Shingo SEMBA ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines ◽  
Differential Effects
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