In situ single molecule imaging of cell membranes: linking basic nanotechniques to cell biology, immunology and medicine

Nanoscale ◽  
2014 ◽  
Vol 6 (21) ◽  
pp. 12229-12249 ◽  
Author(s):  
Jiang Pi ◽  
Hua Jin ◽  
Fen Yang ◽  
Zheng W. Chen ◽  
Jiye Cai
Keyword(s):  
Single Molecule ◽  
Cell Biology ◽  
Cell Membranes ◽  
Single Molecule Imaging

Compact Halo-Ligand-Conjugated Quantum Dots for Multicolored Single-Molecule Imaging of Overcrowding GPCR Proteins on Cell Membranes

Small ◽  
2014 ◽  
Vol 11 (12) ◽  
pp. 1396-1401 ◽  
Author(s):  
Akihito Komatsuzaki ◽  
Tatsuya Ohyanagi ◽  
Yoshikazu Tsukasaki ◽  
Yukihiro Miyanaga ◽  
Masahiro Ueda ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Single Molecule ◽  
Cell Membranes ◽  
Single Molecule Imaging

Construction of Dual-Color Probes with Target-Triggered Signal Amplification for In Situ Single-Molecule Imaging of MicroRNA

ACS Nano ◽  
2020 ◽  
Vol 14 (7) ◽  
pp. 8116-8125
Author(s):  
Binxiao Li ◽  
Yujie Liu ◽  
Yixin Liu ◽  
Tongtong Tian ◽  
Beibei Yang ◽  
...  
Keyword(s):  
Single Molecule ◽  
Signal Amplification ◽  
Single Molecule Imaging ◽  
Dual Color

scholarly journals FISIK: Framework for the Inference of In Situ Interaction Kinetics from Single-Molecule Imaging Data

2019 ◽  
Vol 116 (3) ◽  
pp. 288a
Author(s):  
Luciana R. de Oliveira ◽  
Robel Yirdaw ◽  
Khuloud Jaqaman
Keyword(s):  
Single Molecule ◽  
Single Molecule Imaging ◽  
Imaging Data ◽  
Interaction Kinetics

Large-scale single-molecule imaging aided by artificial intelligence

Microscopy ◽  
2020 ◽  
Vol 69 (2) ◽  
pp. 69-78
Author(s):  
Michio Hiroshima ◽  
Masato Yasui ◽  
Masahiro Ueda
Keyword(s):  
Artificial Intelligence ◽  
Single Molecule ◽  
Cell Biology ◽  
Large Scale ◽  
Molecular Mechanisms ◽  
Imaging System ◽  
Time Frame ◽  
Single Molecule Imaging ◽  
Imaging Analysis ◽  
High Throughput Analysis

Abstract Single-molecule imaging analysis has been applied to study the dynamics and kinetics of molecular behaviors and interactions in living cells. In spite of its high potential as a technique to investigate the molecular mechanisms of cellular phenomena, single-molecule imaging analysis has not been extended to a large scale of molecules in cells due to the low measurement throughput as well as required expertise. To overcome these problems, we have automated the imaging processes by using computer operations, robotics and artificial intelligence (AI). AI is an ideal substitute for expertise to obtain high-quality images for quantitative analysis. Our automated in-cell single-molecule imaging system, AiSIS, could analyze 1600 cells in 1 day, which corresponds to ∼ 100-fold higher efficiency than manual analysis. The large-scale analysis revealed cell-to-cell heterogeneity in the molecular behavior, which had not been recognized in previous studies. An analysis of the receptor behavior and downstream signaling was accomplished within a significantly reduced time frame and revealed the detailed activation scheme of signal transduction, advancing cell biology research. Furthermore, by combining the high-throughput analysis with our previous finding that a receptor changes its behavioral dynamics depending on the presence of a ligand/agonist or inhibitor/antagonist, we show that AiSIS is applicable to comprehensive pharmacological analysis such as drug screening. This AI-aided automation has wide applications for single-molecule analysis.

scholarly journals Single-Molecule Imaging of Wnt3A Protein Diffusion on Living Cell Membranes

2017 ◽  
Vol 113 (12) ◽  
pp. 2762-2767 ◽  
Author(s):  
Anna Lippert ◽  
Agnieszka A. Janeczek ◽  
Alexandre Fürstenberg ◽  
Aleks Ponjavic ◽  
W.E. Moerner ◽  
...  
Keyword(s):  
Single Molecule ◽  
Living Cell ◽  
Cell Membranes ◽  
Single Molecule Imaging ◽  
Protein Diffusion

scholarly journals Imaging: Compact Halo-Ligand-Conjugated Quantum Dots for Multicolored Single-Molecule Imaging of Overcrowding GPCR Proteins on Cell Membranes (Small 12/2015)

Small ◽  
2015 ◽  
Vol 11 (12) ◽  
pp. 1358-1358
Author(s):  
Akihito Komatsuzaki ◽  
Tatsuya Ohyanagi ◽  
Yoshikazu Tsukasaki ◽  
Yukihiro Miyanaga ◽  
Masahiro Ueda ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Single Molecule ◽  
Cell Membranes ◽  
Single Molecule Imaging
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