Continuous production of nitrogen-functionalized graphene nanosheets for catalysis applications

Nanoscale ◽  
2014 ◽  
Vol 6 (21) ◽  
pp. 12758-12768 ◽  
Author(s):  
Kodepelly Sanjeeva Rao ◽  
Jaganathan Senthilnathan ◽  
Jyh-Ming Ting ◽  
Masahiro Yoshimura
Keyword(s):  
Graphene Nanosheets ◽  
Continuous Production ◽  
Functionalized Graphene ◽  
High Quality

A “continuous production” of high-quality nitrogen-functionalized graphene nanosheets directly from graphite and their application to reduction catalysis has been reported.

scholarly journals Homogenous overexpression of the extracellular matrix protein Netrin-1 in a hollow fiber bioreactor

2021 ◽  
Author(s):  
Aniel Moya-Torres ◽  
Monika Gupta ◽  
Fabian Heide ◽  
Natalie Krahn ◽  
Scott Legare ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Hollow Fiber ◽  
Mammalian Cells ◽  
Matrix Protein ◽  
Continuous Production ◽  
Extracellular Matrix Protein ◽  
Close Monitoring ◽  
High Quality ◽  
Biolayer Interferometry ◽  
Hollow Fiber Bioreactor

Abstract The production of recombinant proteins for functional and biophysical studies, especially in the field of structural determination, still represents a challenge as high quality and quantities are needed to adequately perform experiments. This is in part solved by optimizing protein constructs and expression conditions to maximize the yields in regular flask expression systems. Still, work flow and effort can be substantial with no guarantee to obtain improvements. This study presents a combination of workflows that can be used to dramatically increase protein production and improve processing results, specifically for the extracellular matrix protein Netrin-1. This proteoglycan is an axon guidance cue which interacts with various receptors to initiate downstream signaling cascades affecting cell differentiation, proliferation, metabolism, and survival. We were able to produce large glycoprotein quantities in mammalian cells, which were engineered for protein overexpression and secretion into the media using the controlled environment provided by a hollow fiber bioreactor. Close monitoring of the internal bioreactor conditions allowed for stable production over an extended period of time. In addition to this, Netrin-1 concentrations were monitored in expression media through biolayer interferometry which allowed us to increase Netrin-1 media concentrations tenfold over our current flask systems while preserving excellent protein quality and in solution behavior. Our particular combination of genetic engineering, cell culture system, protein purification, and biophysical characterization permitted us to establish an efficient and continuous production of high-quality protein suitable for structural biology studies that can be translated to various biological systems. Key points • Hollow fiber bioreactor produces substantial yields of homogenous Netrin-1 • Biolayer interferometry allows target protein quantitation in expression media • High production yields in the bioreactor do not impair Netrin-1 proteoglycan quality Graphical abstract

Tribological behavior of dodecylamine functionalized graphene nanosheets dispersed engine oil nanolubricants

2019 ◽  
Vol 131 ◽  
pp. 605-619 ◽  
Author(s):  
Gayatri Paul ◽  
Subhasis Shit ◽  
Harish Hirani ◽  
Tapas Kuila ◽  
N.C. Murmu
Keyword(s):  
Tribological Behavior ◽  
Graphene Nanosheets ◽  
Engine Oil ◽  
Functionalized Graphene

scholarly journals High-Performance Thermal Management Nanocomposites: Silver Functionalized Graphene Nanosheets and Multiwalled Carbon Nanotube

Crystals ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 398 ◽  
Author(s):  
Yongcun Zhou ◽  
Xiao Zhuang ◽  
Feixiang Wu ◽  
Feng Liu
Keyword(s):  
Thermal Conductivity ◽  
Carbon Nanotube ◽  
Polymer Composites ◽  
Thermal Management ◽  
High Performance ◽  
Graphene Nanosheets ◽  
Multiwalled Carbon Nanotube ◽  
Processing Unit ◽  
Functionalized Graphene ◽  
Multiwalled Carbon

Polymer composites with high thermal conductivity have a great potential for applications in modern electronics due to their low cost, easy process, and stable physical and chemical properties. Nevertheless, most polymer composites commonly possess unsatisfactory thermal conductivity, primarily because of the high interfacial thermal resistance between inorganic fillers. Herein, we developed a novel method through silver functionalized graphene nanosheets (GNS) and multiwalled carbon nanotube (MWCNT) composites with excellent thermal properties to meet the requirements of thermal management. The effects of composites on interfacial structure and properties of the composites were identified, and the microstructures and properties of the composites were studied as a function of the volume fraction of fillers. An ultrahigh thermal conductivity of 12.3 W/mK for polymer matrix composites was obtained, which is an approximate enhancement of 69.1 times compared to the polyvinyl alcohol (PVA) matrix. Moreover, these composites showed more competitive thermal conductivities compared to untreated fillers/PVA composites applied to the desktop central processing unit, making these composites a high-performance alternative to be used for thermal management.

Functionalized graphene nanosheets with improved dispersion stability and superior paclitaxel loading capacity

2019 ◽  
Vol 173 ◽  
pp. 421-428 ◽  
Author(s):  
Vrushti Kansara ◽  
Rahul Patil ◽  
Rahul Tripathi ◽  
Prafulla K. Jha ◽  
Pratap Bahadur ◽  
...  
Keyword(s):  
Graphene Nanosheets ◽  
Dispersion Stability ◽  
Loading Capacity ◽  
Functionalized Graphene

Damage-Free ALD Blocking Oxide Layer on Functionalized Graphene Nanosheets as Nonvolatile Memories

2019 ◽  
Vol 66 (2) ◽  
pp. 1113-1117 ◽  
Author(s):  
Kai-Ping Chang ◽  
Jer-Chyi Wang ◽  
Han-Hsiang Tai ◽  
Wen-Kuan Yeh ◽  
Kai-Shin Li ◽  
...  
Keyword(s):  
Oxide Layer ◽  
Graphene Nanosheets ◽  
Functionalized Graphene ◽  
Nonvolatile Memories
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