A paradigm shift in the excitation wavelength of upconversion nanoparticles

Nanoscale ◽  
2014 ◽  
Vol 6 (15) ◽  
pp. 8441-8443 ◽  
Author(s):  
Muthu kumara gnanasammandhan Jayakumar ◽  
Niagara Muhammad Idris ◽  
Kai Huang ◽  
Yong Zhang
Keyword(s):  
Paradigm Shift ◽  
Excitation Wavelength ◽  
Upconversion Nanoparticles ◽  
Biological Applications

Shifting the excitation wavelength of upconversion nanoparticles from classical 980 nm to biologically friendlier 800 nm excitation for biological applications.

scholarly journals Upconversion nanoparticles based on rare-earth elements

2019 ◽  
Vol 220 ◽  
pp. 03033
Author(s):  
Dmitrii Zharkov ◽  
Andrey Leontyev ◽  
Artemii Smelev ◽  
Victor Nikiforov ◽  
Vladimir Lobkov ◽  
...  
Keyword(s):  
Experimental Data ◽  
Aqueous Solution ◽  
Rare Earth ◽  
Laser Radiation ◽  
Rare Earth Elements ◽  
Hydrothermal Method ◽  
Water Soluble ◽  
Upconversion Nanoparticles ◽  
Biological Applications ◽  
Intense Luminescence

Using the hydrothermal method, we synthesized water soluble YVO4: Yb, Er nanoparticles with a size less than 10 nm. Nanoparticles exhibit intense luminescence in the green region due to Er3+ ions when excited by laser radiation at a wavelength of 980 nm as a result of the up-conversion process. Bright and stable luminescence also persists in an aqueous solution of nanoparticles. Based on experimental data, it can be argued that the objects obtained are promising in biological applications, as well as up-conversion phosphors.

794 nm excited core–shell upconversion nanoparticles for optical temperature sensing

RSC Advances ◽  
2016 ◽  
Vol 6 (14) ◽  
pp. 11795-11801 ◽  
Author(s):  
Guicheng Jiang ◽  
Shaoshuai Zhou ◽  
Xiantao Wei ◽  
Yonghu Chen ◽  
Changkui Duan ◽  
...  
Keyword(s):  
Temperature Sensors ◽  
Temperature Sensing ◽  
Excitation Wavelength ◽  
Upconversion Nanoparticles ◽  
Core Shell

Nd3+ sensitized core–shell upconversion nanoparticles (UNCPs) are promising candidates for application in optical temperature sensors at a biocompatible excitation wavelength (794 nm).

Dye-sensitized lanthanide-doped upconversion nanoparticles

2017 ◽  
Vol 46 (14) ◽  
pp. 4150-4167 ◽  
Author(s):  
Xindong Wang ◽  
Rashid R. Valiev ◽  
Tymish Y. Ohulchanskyy ◽  
Hans Ågren ◽  
Chunhui Yang ◽  
...  
Keyword(s):  
Excitation Wavelength ◽  
Upconversion Nanoparticles ◽  
Dye Sensitized ◽  
Optical Upconversion

Dye sensitized lanthanide upconversion entails optical upconversion with unprecedented luminescence brightness and broadband excitation wavelength.

P3504Near-Infrared photoactivation via upconversion nanoparticles promotes new advances in cardiac optogenetics toolbox

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
X I Wang ◽  
Y Cheng ◽  
P Rao ◽  
L Wang
Keyword(s):  
Visible Light ◽  
Pulse Duration ◽  
Blue Light ◽  
Upconversion Luminescence ◽  
Luminescence Spectra ◽  
Threshold Power ◽  
Excitation Wavelength ◽  
Upconversion Nanoparticles ◽  
Tissue Penetration ◽  
Nir Light

Abstract Background The optogenetics manipulation of the heart based on the visible light is limited in the therapeutic potential because of the low tissue penetration. Near-infrared (NIR) light has deeper tissue penetration capabilities but radiates at unsuitable wavelengths, while upconversion nanoparticles (UCNPs) absorb NIR light to convert visible light. Purpose We aimed to investigate the efficient NIR control of the rat heart in vivo via UCNPs mediated cardiac optogenetics. Methods Systemic delivery via jugular vein injection of (AAV9-CAG-hChR2 (H134R)-mCherry) were performed in SD rats to achieve sufficient Channelrhodopsin-2 (ChR2) transfer throughout the whole heart. UCNPs of NaYF4:Yb/Tm with optimal excitation wavelength at 975nm were chosen to emit upconverted blue light. Different concentrations of UCNPs cyclohexane solution were embed in composite polydimethylsiloxane films to make flexible substrates for cardiac optogenetics study in open-chest rats (n=3). The UCNPs film was attached to the right ventricle and the 980nm NIR illumination was applied. Results The upconversion luminescence spectra of four concentrations (2.5, 5, 10 and 20mg/ml) of NaYF4:Yb/Tm scanned under 980nm excitation at 0.5w showed similar peaks around 475, 645 and 695nm. Emission intensity increased with the UCNPs concentration (Figure 1). The NIR-upconverted blue light from the freestanding films embedded with 2.5 and 5mg/ml UCNPs failed to capture the heart till the peak output power of the NIR laser, and the hearts were successfully captured and paced by the upconverted blue light from 10 and 20mg/mL UCNPs films (20 pulses in 8Hz with 20ms duration were repeated 3 times with the interval of 1s). However the NIR power was lower on 10mg/mL UCNPs film than the 20mg/mL one (0.93±0.11w vs 1.71±0.75w). Therefore, UCNPs film with concentration of 10mg/mL NaYF4:Yb/Tm were used for efficient cardiac optogenetic pacing by NIR light from a 400um optical fiber. Optogenetics capture of the ventricle was achieved at different NIR power, pulse duration and flash frequency. The strength-duration curve summarized the minimal NIR irradiance power of 8Hz flash required for 100% capture at different pulse duration (2, 5, 10, 20 and 50ms). Notably the longer the pulse duration was, the lower the light intensity required. Furthermore, the increasing flash frequency (6, 7, 8 and 10Hz) of the NIR light setting at 1.66w (2-fold threshold power) and 20ms duration induced sufficient cardiac pacing (Figure 2). The efficient NIR control of the heart Conclusion We demonstrated the successful NIR photo-activation of ChR2 expressed in the heart by the upconverted blue light via UCNPs, which resulted in a flexible UCNPs-assisted cardiac optogenetic approach for optical control of heart activity. We believe that these advances in cardiac optogenetic toolbox not only represent a novel practical application of UCNPs, but also open up new possibilities for remote or tissue penetrating heart control. Acknowledgement/Funding The national natural science foundation of China (81772044)

scholarly journals Excitation-power-dependent upconversion luminescence competition in single β-NaYbF4:Er microcrystal pumped at 808 nm

2021 ◽  
Author(s):  
Maohui Yuan ◽  
Zining Yang ◽  
Xu Yang ◽  
Linxuan Wang ◽  
Rui Wang ◽  
...  
Keyword(s):  
Continuous Wave ◽  
Upconversion Luminescence ◽  
Excitation Power ◽  
Excitation Intensity ◽  
Excitation Wavelength ◽  
Water Molecules ◽  
Biological Applications ◽  
One Pot ◽  
Color Changes ◽  
Particle Level

Abstract Controlling the upconversion luminescence (UCL) intensity ratio, especially pumped at 808 nm, is of fundamental importance in biological applications due to the water molecules exhibiting low absorption at this excitation wavelength. In this work, a series of β-NaYbF4:Er microrods were synthesized by a simple one-pot hydrothermal method and their intense green (545 nm) and red (650 nm) UCL were experimentally investigated based on single particle level under the excitation of 808 nm continuous-wave (CW) laser. Interestingly, the competition between the green and red UCL can be observed in highly Yb3+-doped microcrystals as the excitation intensity gradually increases, which leads to the UCL color changes from green to orange. However, the microcrystals doped with low Yb3+ concentration keep green color which is independent on the excitation power. Further investigations demonstrate that the cross-relaxation (CR) processes between Yb3+ and Er3+ ions result in the UCL competition.

scholarly journals Enzymatic enhancing of triplet–triplet annihilation upconversion by breaking oxygen quenching for background-free biological sensing

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Ling Huang ◽  
Timmy Le ◽  
Kai Huang ◽  
Gang Han
Keyword(s):  
Aqueous Solutions ◽  
Oxygen Depletion ◽  
Upconversion Nanoparticles ◽  
Ambient Atmosphere ◽  
Biological Sensors ◽  
Biological Applications ◽  
Solar Energy Harvesting ◽  
Activity Of Enzymes ◽  
Glucose Oxidation Reaction ◽  
Triplet Triplet Annihilation

AbstractTriplet-triplet annihilation upconversion nanoparticles have attracted considerable interest due to their promises in organic chemistry, solar energy harvesting and several biological applications. However, triplet-triplet annihilation upconversion in aqueous solutions is challenging due to sensitivity to oxygen, hindering its biological applications under ambient atmosphere. Herein, we report a simple enzymatic strategy to overcome oxygen-induced triplet-triplet annihilation upconversion quenching. This strategy stems from a glucose oxidase catalyzed glucose oxidation reaction, which enables rapid oxygen depletion to turn on upconversion in the aqueous solution. Furthermore, self-standing upconversion biological sensors of such nanoparticles are developed to detect glucose and measure the activity of enzymes related to glucose metabolism in a highly specific, sensitive and background-free manner. This study not only overcomes the key roadblock for applications of triplet-triplet annihilation upconversion nanoparticles in aqueous solutions, it also establishes the proof-of-concept to develop triplet-triplet annihilation upconversion nanoparticles as background free self-standing biological sensors.

Recent advances of lanthanide-doped upconversion nanoparticles for biological applications

2019 ◽  
Vol 31 (7) ◽  
pp. 072001 ◽  
Author(s):  
Hui Li ◽  
Xin Wang ◽  
Dingxin Huang ◽  
Guanying Chen
Keyword(s):  
Upconversion Nanoparticles ◽  
Biological Applications ◽  
Recent Advances

scholarly journals Upconversion nanoparticles: synthesis, surface modification and biological applications

2011 ◽  
Vol 7 (6) ◽  
pp. 710-729 ◽  
Author(s):  
Meng Wang ◽  
Gopal Abbineni ◽  
April Clevenger ◽  
Chuanbin Mao ◽  
Shukun Xu
Keyword(s):  
Surface Modification ◽  
Upconversion Nanoparticles ◽  
Biological Applications ◽  
Nanoparticles Synthesis

scholarly journals Engineering Red-Enhanced and Biocompatible Upconversion Nanoparticles

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 284
Author(s):  
Masfer Alkahtani ◽  
Najla Alsofyani ◽  
Anfal Alfahd ◽  
Anas A. Almuqhim ◽  
Fahad A. Almughem ◽  
...  
Keyword(s):  
Optical Properties ◽  
Fluorescent Probes ◽  
Living Cell ◽  
Excitation Wavelength ◽  
Upconversion Nanoparticles ◽  
Core Shell ◽  
Cell Models ◽  
Ion Concentrations ◽  
Fluorescent Markers ◽  
Surface Quenching

The exceptional optical properties of lanthanide-doped upconversion nanoparticles (UCNPs) make them among the best fluorescent markers for many promising bioapplications. To fully utilize the unique advantages of the UCNPs for bioapplications, recent significant efforts have been put into improving the brightness of small UCNPs crystals by optimizing dopant concentrations and utilizing the addition of inert shells to avoid surface quenching effects. In this work, we engineered bright and small size upconversion nanoparticles in a core–shell–shell (CSS) structure. The emission of the synthesized CSS UCNPs is enhanced in the biological transparency window under biocompatible excitation wavelength by optimizing dopant ion concentrations. We also investigated the biosafety of the synthesized CSS UCNP particles in living cell models to ensure bright and non-toxic fluorescent probes for promising bioapplications.

How SIMS microscopy can be used in medicine

1992 ◽  
Vol 50 (2) ◽  
pp. 1602-1603
Author(s):  
Philippe Fragu
Keyword(s):  
Mass Spectrometry ◽  
Biomedical Applications ◽  
Secondary Ion Mass Spectrometry ◽  
Chemical Elements ◽  
Biological Applications ◽  
The Past ◽  
Potential Source ◽  
Secondary Ion ◽  
Chemical Integrity ◽  
Scientific Endeavour

The identification, localization and quantification of intracellular chemical elements is an area of scientific endeavour which has not ceased to develop over the past 30 years. Secondary Ion Mass Spectrometry (SIMS) microscopy is widely used for elemental localization problems in geochemistry, metallurgy and electronics. Although the first commercial instruments were available in 1968, biological applications have been gradual as investigators have systematically examined the potential source of artefacts inherent in the method and sought to develop strategies for the analysis of soft biological material with a lateral resolution equivalent to that of the light microscope. In 1992, the prospects offered by this technique are even more encouraging as prototypes of new ion probes appear capable of achieving the ultimate goal, namely the quantitative analysis of micron and submicron regions. The purpose of this review is to underline the requirements for biomedical applications of SIMS microscopy.Sample preparation methodology should preserve both the structural and the chemical integrity of the tissue.

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