Dynamical insights into the differential characteristics of Mycobacterium avium subsp. paratuberculosis peptide binding to HLA-DRB1 proteins associated with multiple sclerosis

2015 ◽  
Vol 39 (2) ◽  
pp. 1355-1366 ◽  
Author(s):  
Amit Kumar ◽  
Leonardo A. Sechi ◽  
Pierluigi Caboni ◽  
Maria Giovanna Marrosu ◽  
Luigi Atzori ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Mycobacterium Avium ◽  
Peptide Binding ◽  
Mycobacterium Avium Subsp ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Differential Properties

Differential properties of MAP binding to HLA proteins in Sardinian MS patients.

scholarly journals Association of Mycobacterium avium subsp. paratuberculosis with Multiple Sclerosis in Sardinian Patients

PLoS ONE ◽  
2011 ◽  
Vol 6 (4) ◽  
pp. e18482 ◽  
Author(s):  
Davide Cossu ◽  
Eleonora Cocco ◽  
Daniela Paccagnini ◽  
Speranza Masala ◽  
Niyaz Ahmed ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subsp ◽  
Mycobacterium Avium Subsp Paratuberculosis

scholarly journals Association of Mycobacterium avium subsp. paratuberculosis and SLC11A1 polymorphisms in Sardinian multiple sclerosis patients

2013 ◽  
Vol 7 (03) ◽  
pp. 203-207 ◽  
Author(s):  
Davide Cossu ◽  
Speranza Masala ◽  
Eleonora Cocco ◽  
Daniela Paccagnini ◽  
Stefania Tranquilli ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Autoimmune Disease ◽  
Mycobacterium Avium ◽  
Humoral Response ◽  
Mycobacterium Avium Subsp ◽  
Nucleotide Polymorphisms ◽  
Specific Pcr ◽  
Pcr Rflp ◽  
Mycobacterium Avium Subsp Paratuberculosis ◽  
Multiple Sclerosis Patients

Introduction: Recent findings propose that Mycobacterium avium subsp. paratuberculosis (MAP) infection could act as risk factor in favoring multiple sclerosis (MS) progression. SLC11A1 is a gene associated with mycobacterial survival in the host and it may be involved in the induction and maintenance of autoimmune disease. Methodology: In this preliminary study, 100 MS patients and 100 healthy controls (HCs) from Sardinia were enrolled. Eight single nucleotide polymorphisms (SNPs) in the SLC11A gene were searched by PCR RFLP-genotyping. IS900 specie specific PCR was undertaken to search for MAP presence. Indirect ELISA was performed to asses if MS patients displayed a stronger humoral response against MAP2694 protein compared to the HCs. Results: Only rs2276631 SNP was associated with MS. MAP DNA was detected in 23 out of 100 MS patients (23%) and in 7 out of 100 HCs (7%). A strong humoral response against MAP2694 protein was detected in 36% of MS patients and only in 3% of HCs. A correlation between ELISA sero-positivity and the rs2276631 SNP was also found. Conclusion:  Our preliminary results suggest that the Sardinian population might be prone to develop autoimmune disease due to polymorphisms in immunomodulating the SLC11A1 gene, which is important in the immune response against intracellular bacteria such as MAP.

Are Mycobacterium avium subsp. paratuberculosis and Epstein–Barr virus triggers of multiple sclerosis in Sardinia?

2012 ◽  
Vol 18 (8) ◽  
pp. 1181-1184 ◽  
Author(s):  
Davide Cossu ◽  
Speranza Masala ◽  
Eleonora Cocco ◽  
Daniela Paccagnini ◽  
Jessica Frau ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Mycobacterium Avium ◽  
Epstein Barr Virus ◽  
Mycobacterium Avium Subsp ◽  
Healthy Controls ◽  
Humoral Immune ◽  
Barr Virus ◽  
Protein Map ◽  
Epstein Barr ◽  
Mycobacterium Avium Subsp Paratuberculosis

Sardinia acts as an ideal setting for multiple sclerosis (MS) studies because its prevalence of MS is one of the highest worldwide. Several pathogens have been investigated amongst 119 Sardinian MS patients and 117 healthy controls to determine whether they might have a role in triggering MS in genetically predisposed individuals. Mycobacterium avium subsp. paratuberculosis (MAP) and Epstein Barr virus DNA were detected in 27.5% and 17.3%, respectively, of the MS patients. Moreover an extremely high humoral immune response against MAP recombinant protein MAP FprB (homologous to human myelin P0) was observed, whereas no significant results were found against Mycobacterium tuberculosis FprA and Helicobacter pylori HP986 protein.

scholarly journals Association between Lipoprotein Levels and Humoral Reactivity to Mycobacterium avium subsp. paratuberculosis in Multiple Sclerosis, Type 1 Diabetes Mellitus and Rheumatoid Arthritis

2019 ◽  
Vol 7 (10) ◽  
pp. 423 ◽  
Author(s):  
Bo ◽  
Arru ◽  
Niegowska ◽  
Erre ◽  
Manchia ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Multiple Sclerosis ◽  
Type 1 Diabetes ◽  
Mycobacterium Avium ◽  
Bacterial Infections ◽  
Molecular Mimicry ◽  
Serum Lipoprotein ◽  
Mycobacterium Avium Subsp ◽  
Mycobacterium Avium Subsp Paratuberculosis

Environmental factors such as bacterial infections may play an important role in the development of autoimmune diseases. Mycobacterium avium subsp. paratuberculosis (MAP) is an obligate pathogen of ruminants able to use the host’s cholesterol for survival into macrophages and has been associated with multiple sclerosis (MS), type 1 diabetes (T1DM) and rheumatoid arthritis (RA) through a molecular mimicry mechanism. Here, we aimed at investigating the correlation between humoral reactivity against MAP and serum lipoprotein levels in subjects at T1DM risk (rT1DM) grouped by geographical background and in patients affected by MS or RA. Our results showed significant differences in HDL, LDL/VLDL and Total Cholesterol (TC) levels between patients and healthy controls (p < 0.0001). Patients positive to anti-MAP Abs (MAP+) had lower HDL levels in comparison with Abs negative (MAP-) subjects, while opposite trends were found for LDL/VLDL concentrations (p < 0.05). TC levels varied between MAP+ and MAP- patients in all three assessed diseases. These findings suggest the implication of anti-MAP Abs in fluctuations of lipoprotein levels highlighting a possible link with cardiovascular disease. Further studies will be needed to confirm these results in larger groups.

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