A label-free conjugated polymer-based fluorescence assay for the determination of adenosine triphosphate and alkaline phosphatase

2014 ◽  
Vol 38 (9) ◽  
pp. 4574-4579 ◽  
Author(s):  
Yanan Li ◽  
Yan Li ◽  
Xinyan Wang ◽  
Xingguang Su
Keyword(s):  
Alkaline Phosphatase ◽  
Adenosine Triphosphate ◽  
Conjugated Polymer ◽  
Fluorescence Assay ◽  
Label Free ◽  
Quenching Effect ◽  
Hydrolysis Of

A sensor was developed based on the quenching effect of Cu2+ on PPESO3 and the hydrolysis of ATP by ALP.

Carbon Quantum Dots-Based Recyclable Real-Time Fluorescence Assay for Alkaline Phosphatase with Adenosine Triphosphate as Substrate

2015 ◽  
Vol 87 (5) ◽  
pp. 2966-2973 ◽  
Author(s):  
Zhaosheng Qian ◽  
Lujing Chai ◽  
Cong Tang ◽  
Yuanyuan Huang ◽  
Jianrong Chen ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Alkaline Phosphatase ◽  
Real Time ◽  
Adenosine Triphosphate ◽  
Carbon Quantum Dots ◽  
Fluorescence Assay

An Automated Alkaline Phosphatase Assay with Phenolphthalein Monophosphate as Substrate

1967 ◽  
Vol 13 (4) ◽  
pp. 281-289 ◽  
Author(s):  
Kirsten Hviid
Keyword(s):  
Alkaline Phosphatase ◽  
Normal Values ◽  
Serum Alkaline Phosphatase ◽  
Colorimetric Determination ◽  
Alkaline Phosphatase Assay ◽  
Phosphatase Assay ◽  
Hydrolysis Of

Abstract The manual procedure of Babson et al. (1) for the determination of serum alkaline phosphatase has been automated. The assay is based on the colorimetric determination of phenolphthalein formed on hydrolysis of phenolphthalein monophosphate. The procedure utilizes 0.16 ml. of serum without dialysis. Blanks are required only for turbid sera. Results are compared with those obtained by the manual procedure, and data relating to sample interaction, precision, blank values, and normal values are presented.

A turn-on fluorescence assay of alkaline phosphatase activity using a DNA–silver nanocluster probe

2018 ◽  
Vol 42 (6) ◽  
pp. 4331-4336 ◽  
Author(s):  
Changbei Ma ◽  
Haisheng Liu ◽  
Kefeng Wu ◽  
Mingjian Chen ◽  
Hailun He ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Fluorescence Assay ◽  
Label Free ◽  
Silver Nanocluster ◽  
Turn On

A label-free fluorescence assay has been developed for the detection of alkaline phosphatase based on DNA–silver nanocluster probes.

Cerium(iii)-directed assembly of glutathione-capped gold nanoclusters for sensing and imaging of alkaline phosphatase-mediated hydrolysis of adenosine triphosphate

Nanoscale ◽  
2018 ◽  
Vol 10 (37) ◽  
pp. 17691-17698 ◽  
Author(s):  
Jyun-Guo You ◽  
Chi-Yu Lu ◽  
A. Santhana Krishna Kumar ◽  
Wei-Lung Tseng
Keyword(s):  
Alkaline Phosphatase ◽  
Adenosine Triphosphate ◽  
Gold Nanoclusters ◽  
Directed Assembly ◽  
Gold Clusters ◽  
Enzymatic Reactions ◽  
Aggregation Induced Emission ◽  
Emission Enhancement ◽  
Hydrolysis Of

The aggregation-induced emission enhancement of glutathione-capped gold clusters triggered by cerium(iii) is well-suited for the detection of adenosine triphosphate-related enzymatic reactions.

scholarly journals Determination of L-Argininamide Based on Water-Soluble Fluorescent Conjugated Polymer-Aptamer

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Hongliang Guan ◽  
Zhike He
Keyword(s):  
Conjugated Polymer ◽  
Specific Binding ◽  
Water Soluble ◽  
Dna Sensor ◽  
Label Free ◽  
Binding Reaction ◽  
Molecular Sensors ◽  
Free Dna ◽  
Highly Sensitive

Water-soluble fluorescent conjugated polymer is a promising material which could be used as an optical platform in highly sensitive molecular sensors. In this paper, a simple label-free DNA sensor, which consisted of a poly(3-alkoxy-4-methylthiophene) and an aptamer, was used to detect L-argininamide (L-Arm). Due to the specific binding reaction between L-Arm and its aptamer, the proposed method can easily determinate the L-Arm through the recovery of fluorescence without any modification. Other ions or similar molecules had little effect on the detection. Moreover, there was a linear relationship between fluorescence intensity and the concentration of L-Arm. The detection limit of L-Arm was as low as 4.7 nM.

Determination of gamma-globulin at nanogram levels by its quenching effect on the fluorescence of a red emitting conjugated polymer

2015 ◽  
Vol 39 (6) ◽  
pp. 4551-4555 ◽  
Author(s):  
Ping Zhang ◽  
Shujuan Zhuo ◽  
Lilin Sun ◽  
Ping Zhang ◽  
Changqing Zhu
Keyword(s):  
Serum Albumin ◽  
Conjugated Polymer ◽  
Gamma Globulin ◽  
Fluorescence Sensor ◽  
Quenching Effect

A novel red emitting fluorescence sensor has been constructed and used for the selective assay of gamma-globulin in the presence of serum albumin.

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