Preparation of multicolor emitting carbon dots for HeLa cell imaging

2014 ◽  
Vol 38 (12) ◽  
pp. 6152-6160 ◽  
Author(s):  
Vaibhavkumar N. Mehta ◽  
Sanjay Jha ◽  
Rakesh Kumar Singhal ◽  
Suresh Kumar Kailasa
Keyword(s):  
Hela Cell ◽  
Fluorescent Probes ◽  
Hela Cells ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Confocal Laser ◽  
Microscopic Images

Confocal laser microscopic images of HeLa cells using CDs as fluorescent probes.

Pyridine derivative-induced fluorescence in multifunctional modified carbon dots and their application in thermometers

2017 ◽  
Vol 5 (21) ◽  
pp. 3964-3969 ◽  
Author(s):  
Mumei Han ◽  
Liping Wang ◽  
Liang Bai ◽  
Yunjie Zhou ◽  
Yue Sun ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Pyridine Derivative

NS-Cdots can be used as fluorescent probes for cell imaging and as effective thermometers.

Boranil dye based “turn-on” fluorescent probes for detection of hydrogen peroxide and their cell imaging application

RSC Advances ◽  
2016 ◽  
Vol 6 (89) ◽  
pp. 85838-85843 ◽  
Author(s):  
Jayabalan Shanmugapriya ◽  
Kandasamy Rajaguru ◽  
Gandhi Sivaraman ◽  
Shanmugam Muthusubramanian ◽  
Nattamai Bhuvanesh
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probes ◽  
Hela Cells ◽  
Cell Imaging ◽  
Physiological Conditions ◽  
Turn On ◽  
Imaging Application

The synthesis of boranil dye fluorescent probes for the detection of hydrogen peroxide has been described. The probes have been successfully applied for imaging of H2O2 in HeLa cells under physiological conditions.

scholarly journals Structural Analysis of NaYF4 Solution Processed Nanoparticles for Hela Cell Studies

2020 ◽  
Vol 7 (1) ◽  
pp. 45-50
Author(s):  
Cliff Orori Mosiori ◽  
John. Maera
Keyword(s):  
Structural Analysis ◽  
Hela Cell ◽  
Fluorescent Probes ◽  
Hela Cells ◽  
Incubation Time ◽  
Spectral Properties ◽  
Wide Range ◽  
Incubation Periods ◽  
Theranostic Agents ◽  
Concentration Levels

The NaYF4 nanoparticles were prepared and analyzed. Its structural analysis confirmed the formation of nanocrystals of desired sizes and spectral properties that can be incorporated into Hela cell studies. The internalization of NaYF4 nanoparticles in HeLa cells was determined at different nanoparticles concentrations and for incubation periods from 3 to 24 hours using various techniques. The images revealed a redistribution of nanoparticles inside the cell that increased with incubation time, concentration levels, and depended on the presence of the transfection factor. The study identified factors responsible for effective endocytosis of the NaYF4 nanoparticles to HeLa cells. Thus this procedure or method could be applied to investigate a wide range of future “smart” theranostic agents that may result in be very promising fluorescent probes for imaging real-time cellular dynamics.

Photoluminescence of carbon dots and their applications in Hela cell imaging and Fe3+ ion detection

2017 ◽  
Vol 52 (17) ◽  
pp. 9979-9989 ◽  
Author(s):  
Lin Ge ◽  
Hailong Yu ◽  
Haitao Ren ◽  
Bo Shi ◽  
Qian Guo ◽  
...  
Keyword(s):  
Hela Cell ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Ion Detection

scholarly journals Structural Analysis of NaYF4 Solution Processed Nanoparticles for Hela Cell Studies

2020 ◽  
Vol 7 (2) ◽  
pp. 87-92
Author(s):  
Cliff Orori Mosiori ◽  
John. Maera
Keyword(s):  
Structural Analysis ◽  
Hela Cell ◽  
Fluorescent Probes ◽  
Hela Cells ◽  
Incubation Time ◽  
Spectral Properties ◽  
Wide Range ◽  
Incubation Periods ◽  
Theranostic Agents ◽  
Concentration Levels

The NaYF4 nanoparticles were prepared and analyzed. Its structural analysis confirmed the formation of nanocrystals of desired sizes and spectral properties that can be incorporated into Hela cell studies. The internalization of NaYF4 nanoparticles in HeLa cells was determined at different nanoparticles concentrations and for incubation periods from 3 to 24 hours using various techniques. The images revealed a redistribution of nanoparticles inside the cell that increased with incubation time, concentration levels, and depended on the presence of the transfection factor. The study identified factors responsible for effective endocytosis of the NaYF4 nanoparticles to HeLa cells. Thus this procedure or method could be applied to investigate a wide range of future “smart” theranostic agents that may result in be very promising fluorescent probes for imaging real-time cellular dynamics.

Synthesis of azonia cyanine derivatives as NIR fluorescent probes for nucleic acid detection and cell imaging

2019 ◽  
Vol 11 (28) ◽  
pp. 3523-3531
Author(s):  
Yuan Chen ◽  
Xue-Rui Wei ◽  
Ru Sun ◽  
Yu-Jie Xu ◽  
Jian-Jeng Ge
Keyword(s):  
Nucleic Acid ◽  
Fluorescent Probes ◽  
Hela Cells ◽  
Cell Imaging ◽  
Cyanine Dyes ◽  
Nucleic Acid Detection

A series of azonia cyanine dyes were designed and synthesized for nucleic acid detection and nucleolus imaging in fixed HeLa cells.

scholarly journals CHARACTERISTIC OF APOPTOSIS AND EXPRESSION OF GENES-RELATED APOPTOSIS (c-myc, c-erb AND c-fos ONCOGENE) IN HeLa CELL LINES AFTER EXPOSURE BY NEEM (Azadirachta indica A.Juss)

2015 ◽  
Vol 1 (2) ◽  
pp. 116
Author(s):  
Dessy Arisanty ◽  
Zolkapli Eshak ◽  
Fauziah Othman ◽  
Asmah Rahmat ◽  
Abdah M.D. Akim ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Hela Cell ◽  
Cancer Cells ◽  
Azadirachta Indica ◽  
Hela Cells ◽  
Incubation Time ◽  
Confocal Laser ◽  
Hela Cell Lines ◽  
Neem Leaves ◽  
Cervical Cancer Cells

 ABSTRACT Azadirachta indica A. Juss is a medicinal plant commonly known as neem. The effect of neem leaves extract on cervical cancer cells, however, has never been studied. Due to the lack of information, this study was conducted to determine the effect of neem leaves extract on cervical cancer (HeLa) cell growth. In vitro cytotoxicity effect of ethanolic neem extract indicated the presence of cytotoxicity activity of the extract against HeLa cells with IC50 of  30.0 μg/mL. The morphological changes under confocal laser scanning microscope (CLSM) on HeLa cells were cell shrinkage and membrane blebbing. There were also cells with condensed nucleus and few cells have fragmented nucleus, and finally formed apoptotic body. Control cells showed a clear cytoplasm and centrally placed nucleus and no cells exhibited any apoptotic features. Appearance of apoptotic cells under scanning electron microscope (SEM) are indentations, blebs and hole on cell surface and disintegration of cell. The controls remained morphologically normal. Apoptotic features of the cells are widely seen with longer incubation time while 24 hours incubation time, it is scarcely seen. The RT-PCR product showed that the c-erb gene expression was expressed in both treated and untreated HeLa cells. Contrary, the the c-myc and c-fos oncogenes on  HeLa cells which exposed to A. indica  EtOH extract were significantly decreased.  Thus, the results from this study strongly suggest that the ethanolic extract of A. indica may contain bioactive compound(s) that caused cervical cancer cells, HeLa cell death by apoptosis mechanism and lead to succession of discovering new alternative treatment for cervical cancer.  Keywords : cytotoxic, apoptosis genes, oncogenes, neem

Photoluminescence of carbon dots prepared by ball milling and their application in Hela cell imaging

2019 ◽  
Vol 125 (9) ◽  
Author(s):  
Lin Ge ◽  
Guangkuo Hu ◽  
Bo Shi ◽  
Qian Guo ◽  
Lu Li ◽  
...  
Keyword(s):  
Hela Cell ◽  
Ball Milling ◽  
Carbon Dots ◽  
Cell Imaging
Sign in / Sign up

Export Citation Format

Share Document