Mapping the protein profile involved in the biotransformation of organoarsenicals using an arsenic metabolizing bacterium

Metallomics ◽  
2014 ◽  
Vol 6 (10) ◽  
pp. 1958-1969 ◽  
Author(s):  
John A. Thomas ◽  
Peter Chovanec ◽  
John F. Stolz ◽  
Partha Basu
Keyword(s):  
Proteomic Analysis ◽  
Protein Profile ◽  
Insight Into

Insight into the organoarsenic metabolism of Alkaliphilus oremlandii OhILAs by comprehensive proteomic analysis.

Quantitative proteomic analysis of global effect of LLL12 on U87 cell's proteome: An insight into the molecular mechanism of LLL12

2015 ◽  
Vol 113 ◽  
pp. 127-142 ◽  
Author(s):  
Rekha Jain ◽  
Prajakta Kulkarni ◽  
Snigdha Dhali ◽  
Srikanth Rapole ◽  
Sanjeeva Srivastava
Keyword(s):  
Molecular Mechanism ◽  
Proteomic Analysis ◽  
Global Effect ◽  
Quantitative Proteomic Analysis ◽  
Insight Into

scholarly journals Proteomic analysis revealed the altered kidney protein profile of a Cyld knockout mouse model

2015 ◽  
Vol 14 (2) ◽  
pp. 5970-5978 ◽  
Author(s):  
Y. Zhao ◽  
Y. Zhang ◽  
H.B. Song ◽  
F. Wu ◽  
X.L. Wang ◽  
...  
Keyword(s):  
Mouse Model ◽  
Proteomic Analysis ◽  
Knockout Mouse ◽  
Protein Profile ◽  
Knockout Mouse Model

scholarly journals Comparative proteomic analysis of rats subjected to water immersion and restraint stress as an insight into gastric ulcers

2017 ◽  
Vol 16 (4) ◽  
pp. 5425-5433 ◽  
Author(s):  
Zheng-Rong Zhou ◽  
Pan Huang ◽  
Guang-Hao Song ◽  
Zhuang Zhang ◽  
Ke An ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Restraint Stress ◽  
Water Immersion ◽  
Gastric Ulcers ◽  
Comparative Proteomic Analysis ◽  
Insight Into

scholarly journals The Anti-Apoptotic Effect of ASC-Exosomes in an In Vitro ALS Model and Their Proteomic Analysis

Cells ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 1087 ◽  
Author(s):  
Bonafede ◽  
Brandi ◽  
Manfredi ◽  
Scambi ◽  
Schiaffino ◽  
...  
Keyword(s):  
Stem Cells ◽  
Beneficial Effect ◽  
Proteomic Analysis ◽  
In Vitro Model ◽  
Neuroprotective Effect ◽  
Protein Profile ◽  
Cell Based Therapy ◽  
Apoptotic Effect ◽  
Vitro Model

Stem cell therapy represents a promising approach in the treatment of several neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). The beneficial effect of stem cells is exerted by paracrine mediators, as exosomes, suggesting a possible potential use of these extracellular vesicles as non-cell based therapy. We demonstrated that exosomes isolated from adipose stem cells (ASC) display a neuroprotective role in an in vitro model of ALS. Moreover, the internalization of ASC-exosomes by the cells was shown and the molecules and the mechanisms by which exosomes could exert their beneficial effect were addressed. We performed for the first time a comprehensive proteomic analysis of exosomes derived from murine ASC. We identified a total of 189 proteins and the shotgun proteomics analysis revealed that the exosomal proteins are mainly involved in cell adhesion and negative regulation of the apoptotic process. We correlated the protein content to the anti-apoptotic effect of exosomes observing a downregulation of pro-apoptotic proteins Bax and cleaved caspase-3 and upregulation of anti-apoptotic protein Bcl-2 α, in an in vitro model of ALS after cell treatment with exosomes. Overall, this study shows the neuroprotective effect of ASC-exosomes after their internalization and their global protein profile, that could be useful to understand how exosomes act, demonstrating that they can be employed as therapy in neurodegenerative diseases.

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