Serum metabolomics analysis reveals impaired lipid metabolism in rats after oral exposure to benzo(a)pyrene

2015 ◽  
Vol 11 (3) ◽  
pp. 753-759 ◽  
Author(s):  
Xiaoxue Wang ◽  
Jie Zhang ◽  
Qingyu Huang ◽  
Ambreen Alamdar ◽  
Meiping Tian ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Metabolic Profiling ◽  
Oral Exposure ◽  
Metabolomics Study ◽  
Serum Metabolomics ◽  
Metabolomics Analysis

A metabolomics study was conducted to unveil the metabolic profiling of rats exposed to benzo(a)pyrene, and twelve differentiated metabolites were identified.

scholarly journals Metabolomics Research Reveals the Mechanism of Action of Astragalus Polysaccharide in Rats with Digestive System Disorders

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3333 ◽  
Author(s):  
Huanjun Wang ◽  
Ana Liu ◽  
Wenxiao Zhao ◽  
Haijun Zhao ◽  
Lili Gong ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Amino Acid ◽  
Metabolic Pathways ◽  
Digestive System ◽  
Weight Bearing ◽  
High Fat ◽  
Low Protein ◽  
Astragalus Polysaccharide ◽  
Serum Metabolomics ◽  
Metabolomics Analysis

With the diversity of modern dietary lifestyles, digestive system disorders (DSD) have become a frequently occurring disease in recent years. Astragalus polysaccharide (APS) is a homogeneous polysaccharide extracted from Astragalus, which might ameliorate the digestive and absorptive functions. However, the treatment mechanisms remain unclear. In this study, rats with DSD were fed a high-fat–low-protein diet and subjected to weight-bearing swimming until exhaustion. When body weight and autonomous activities of the rats decreased, they were administered APS. After two weeks, serum metabolomics analysis based on LC-MS was performed to validate the therapeutic effect of APS and explore its mechanism. APS pharmacodynamics was determined in this study, and serum metabolomics analysis discovered and identified 16 significant, differentially produced metabolites involved in energy, amino acid, and lipid metabolism, including citric acid, lactic acid, alanine, phosphatidylcholine, phenylalanine. After treatment with APS, the levels of the above small-molecule metabolites were reversed. Our results show the efficacy of APS in DSD treatment through the regulation of perturbed metabolic pathways related to energy, amino acid, and lipid metabolism.

scholarly journals A Prospective Targeted Serum Metabolomics Study of Pancreatic Cancer in Postmenopausal Women

2019 ◽  
Vol 12 (4) ◽  
pp. 237-246 ◽  
Author(s):  
Li Jiao ◽  
Suman Maity ◽  
Cristian Coarfa ◽  
Kimal Rajapakshe ◽  
Liang Chen ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Postmenopausal Women ◽  
Metabolomics Study ◽  
Serum Metabolomics

Effect of oral exposure to titanium dioxide nanoparticles on lipid metabolism in Sprague-Dawley rats

Nanoscale ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 5973-5986 ◽  
Author(s):  
Zhangjian Chen ◽  
Shuo Han ◽  
Pai Zheng ◽  
Di Zhou ◽  
Shupei Zhou ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Lipid Metabolism ◽  
Titanium Dioxide Nanoparticles ◽  
Oral Exposure ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

The present study investigated the effect of oral exposure to TiO2 NPs on lipid metabolism by serum lipidomics.

Metabolomics analysis revealed significantly higher synovial Phe/Tyr ratio in reactive arthritis and undifferentiated spondyloarthropathy

Rheumatology ◽  
2019 ◽  
Vol 59 (7) ◽  
pp. 1587-1590
Author(s):  
Hafis Muhammed ◽  
Dinesh Kumar ◽  
Durgesh Dubey ◽  
Sandeep Kumar ◽  
Smriti Chaurasia ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Reactive Arthritis ◽  
Nmr Spectra ◽  
Regression Coefficient ◽  
Classification Criteria ◽  
Metabolomics Study ◽  
Metabolomics Analysis

Abstract Objective To compare the synovial phenylalanine/tyrosine (Phe/Tyr) ratio between ReA/uSpA and RA and OA by NMR spectroscopy. Methods Paired SF and serum of 30 patients with ReA/uSpA were collected and analysed using a 1D 1H Carr Purcell Meiboom Gill NMR spectra recorded on 800 MHz NMR spectrometer equipped with a TCI Cryoprobe (at 300 K). Phe and Tyr were quantified. SF from 25 patients with RA fulfilling ACR classification criteria and 21 patients with OA were taken as inflammatory and non-inflammatory controls. Results The synovial Phe/Tyr ratio was significantly higher in ReA/uSpA compared with RA and OA. Synovial Phe/Tyr ratios were comparable in RA and OA patients. Compared with serum, the Phe/Tyr was significantly higher in the SF in ReA/uSpA. The Phe/Tyr ratio was also found to be positively correlated between serum and SF samples, with a regression coefficient (r2) of 0.287. Conclusions This NMR-based metabolomics study demonstrates that the synovial Phe/Tyr ratio is specifically elevated in ReA/uSpA.

scholarly journals Lomatogonium Rotatum for Treatment of Acute Liver Injury in Mice: A Metabolomics Study

Metabolites ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 227 ◽  
Author(s):  
Renhao Chen ◽  
Qi Wang ◽  
Lanjun Zhao ◽  
Shilin Yang ◽  
Zhifeng Li ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Linoleic Acid ◽  
Liver Injury ◽  
Acid Metabolism ◽  
Acute Liver Injury ◽  
Tca Cycle ◽  
Arachidonic Acid Metabolism ◽  
Hepatoprotective Effect ◽  
Metabolomics Study ◽  
Metabolomics Analysis

Lomatogonium rotatum (L.) Fries ex Nym (LR) is used as a traditional Mongolian medicine to treat liver and bile diseases. This study aimed to investigate the hepatoprotective effect of LR on mice with CCl4-induced acute liver injury through conventional assays and metabolomics analysis. This study consisted of male mice (n = 23) in four groups (i.e., control, model, positive control, and LR). The extract of whole plant of LR was used to treat mice in the LR group. Biochemical and histological assays (i.e., serum levels of alanine transaminase (ALT) and aspartate transaminase (AST), and histological changes of liver tissue) were used to evaluate LR efficacy, and metabolomics analysis based on GC-MS and LC-MS was conducted to reveal metabolic changes. The conventional analysis and metabolomic profiles both suggested that LR treatment could protect mice against CCl4-induced acute liver injury. The affected metabolic pathways included linoleic acid metabolism, α-linolenic acid metabolism, arachidonic acid metabolism, CoA biosynthesis, glycerophospholipid metabolism, the TCA cycle, and purine metabolism. This study identified eight metabolites, including phosphopantothenic acid, succinic acid, AMP, choline, glycerol 3-phosphate, linoleic acid, arachidonic acid, and DHA, as potential biomarkers for evaluating hepatoprotective effect of LR. This metabolomics study may shed light on possible mechanisms of hepatoprotective effect of LR.

scholarly journals A serum metabolomics study of patients with nAMD in response to anti-VEGF therapy

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yan Gao ◽  
Yi Chong Kelvin Teo ◽  
Roger W. Beuerman ◽  
Tien Yin Wong ◽  
Lei Zhou ◽  
...  
Keyword(s):  
Anti Vegf ◽  
Metabolomics Study ◽  
Serum Metabolomics
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