High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system

Nobuaki Takemori; Ayako Takemori; Kazuhiro Matsuoka; Ryo Morishita; Natsuki Matsushita; Masato Aoshima; Hiroyuki Takeda; Tatsuya Sawasaki; Yaeta Endo; Shigeki Higashiyama

doi:10.1039/c4mb00556b

High-throughput synthesis of stable isotope-labeled transmembrane proteins for targeted transmembrane proteomics using a wheat germ cell-free protein synthesis system

Molecular BioSystems ◽

10.1039/c4mb00556b ◽

2015 ◽

Vol 11 (2) ◽

pp. 361-365 ◽

Cited By ~ 14

Author(s):

Nobuaki Takemori ◽

Ayako Takemori ◽

Kazuhiro Matsuoka ◽

Ryo Morishita ◽

Natsuki Matsushita ◽

...

Keyword(s):

Protein Synthesis ◽

Stable Isotope ◽

High Throughput ◽

Wheat Germ ◽

Transmembrane Proteins ◽

Synthesis System ◽

System P ◽

Novel Strategy ◽

Protein Synthesis System ◽

Cell Free Protein Synthesis

A novel strategy for quantitating transmembrane proteome.

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Detection of structural changes in a cofactor binding protein by using a wheat germ cell-free protein synthesis system coupled with unnatural amino acid probing

Proteins Structure Function and Bioinformatics ◽

10.1002/prot.21341 ◽

2007 ◽

Vol 67 (3) ◽

pp. 643-652 ◽

Cited By ~ 18

Author(s):

Masato Abe ◽

Satoshi Ohno ◽

Takashi Yokogawa ◽

Takeshi Nakanishi ◽

Fumio Arisaka ◽

...

Keyword(s):

Protein Synthesis ◽

Amino Acid ◽

Germ Cell ◽

Wheat Germ ◽

Structural Changes ◽

Unnatural Amino Acid ◽

Synthesis System ◽

Cofactor Binding ◽

Protein Synthesis System ◽

Cell Free Protein Synthesis

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A cell-free protein synthesis system for high-throughput proteomics

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.232580399 ◽

2002 ◽

Vol 99 (23) ◽

pp. 14652-14657 ◽

Cited By ~ 337

Author(s):

T. Sawasaki ◽

T. Ogasawara ◽

R. Morishita ◽

Y. Endo

Keyword(s):

Protein Synthesis ◽

High Throughput ◽

Synthesis System ◽

Free Protein ◽

A Cell ◽

Protein Synthesis System ◽

Cell Free Protein Synthesis

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Stabilization effect of zeolite on DHFR mRNA in a wheat germ cell-free protein synthesis system

Journal of Bioscience and Bioengineering ◽

10.1016/s1389-1723(00)88736-8 ◽

2000 ◽

Vol 89 (2) ◽

pp. 193-195 ◽

Cited By ~ 5

Author(s):

Gyoo Yeol Jung ◽

Eun Yeol Lee ◽

You-Eil Kim ◽

Byeong Woo Jung ◽

Sang Hyeon Kang ◽

...

Keyword(s):

Protein Synthesis ◽

Germ Cell ◽

Wheat Germ ◽

Synthesis System ◽

Free Protein ◽

Stabilization Effect ◽

Protein Synthesis System ◽

Cell Free Protein Synthesis

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Genome-scale, biochemical annotation method based on the wheat germ cell-free protein synthesis system

Phytochemistry ◽

10.1016/j.phytochem.2004.04.023 ◽

2004 ◽

Vol 65 (11) ◽

pp. 1549-1555 ◽

Cited By ~ 36

Author(s):

Tatsuya Sawasaki ◽

Yoshinori Hasegawa ◽

Ryo Morishita ◽

Motoaki Seki ◽

Kazuo Shinozaki ◽

...

Keyword(s):

Protein Synthesis ◽

Germ Cell ◽

Wheat Germ ◽

Synthesis System ◽

Free Protein ◽

Annotation Method ◽

Genome Scale ◽

Protein Synthesis System ◽

Cell Free Protein Synthesis

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The wheat germ cell-free protein synthesis system: A key tool for novel malaria vaccine candidate discovery

Acta Tropica ◽

10.1016/j.actatropica.2009.10.024 ◽

2010 ◽

Vol 114 (3) ◽

pp. 171-176 ◽

Cited By ~ 49

Author(s):

Takafumi Tsuboi ◽

Satoru Takeo ◽

Thangavelu U. Arumugam ◽

Hitoshi Otsuki ◽

Motomi Torii

Keyword(s):

Protein Synthesis ◽

Germ Cell ◽

Wheat Germ ◽

Malaria Vaccine ◽

Vaccine Candidate ◽

Synthesis System ◽

System A ◽

Malaria Vaccine Candidate ◽

Protein Synthesis System ◽

Cell Free Protein Synthesis

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Preparation of Functional Ecdysteroid Receptor Proteins (EcR and USP) Using a Wheat Germ Cell-Free Protein Synthesis System

Journal of Pesticide Science ◽

10.1584/jpestics.29.189 ◽

2004 ◽

Vol 29 (3) ◽

pp. 189-194 ◽

Cited By ~ 4

Author(s):

Chieka Minakuchi ◽

Yoshiaki Nakagawa ◽

Yoshihiro Soya ◽

Hisashi Miyagawa

Keyword(s):

Protein Synthesis ◽

Germ Cell ◽

Wheat Germ ◽

Synthesis System ◽

Ecdysteroid Receptor ◽

Receptor Proteins ◽

Free Protein ◽

Protein Synthesis System ◽

Cell Free Protein Synthesis

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Faculty Opinions recommendation of A cell-free protein synthesis system for high-throughput proteomics.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1010283.148407 ◽

2002 ◽

Author(s):

Sheila McCormick

Keyword(s):

Protein Synthesis ◽

High Throughput ◽

Synthesis System ◽

Free Protein ◽

A Cell ◽

Protein Synthesis System ◽

Cell Free Protein Synthesis

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The Wheat Germ Cell-Free Protein Synthesis System

Cell-Free Protein Synthesis ◽

10.1002/9783527622702.ch7 ◽

2008 ◽

pp. 111-139 ◽

Cited By ~ 2

Author(s):

Tatsuya Sawasaki ◽

Yaeta Endo

Keyword(s):

Protein Synthesis ◽

Germ Cell ◽

Wheat Germ ◽

Synthesis System ◽

Free Protein ◽

Protein Synthesis System ◽

Cell Free Protein Synthesis

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1P024 A highly efficient isotopic labeled protein by using wheat germ cell-free protein synthesis system and an application to NMR technologies

Seibutsu Butsuri ◽

10.2142/biophys.45.s37_4 ◽

2005 ◽

Vol 45 (supplement) ◽

pp. S37

Author(s):

A. Iihara ◽

Y. Nara ◽

M. Kitano ◽

K. Matsubara ◽

T. Kohno

Keyword(s):

Protein Synthesis ◽

Germ Cell ◽

Wheat Germ ◽

Synthesis System ◽

Free Protein ◽

Highly Efficient ◽

Protein Synthesis System ◽

Cell Free Protein Synthesis

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The ligand binding ability of dopamine D1 receptors synthesized using a wheat germ cell-free protein synthesis system with liposomes

European Journal of Pharmacology ◽

10.1016/j.ejphar.2014.10.011 ◽

2014 ◽

Vol 745 ◽

pp. 117-122 ◽

Cited By ~ 11

Author(s):

Eiji Arimitsu ◽

Tomio Ogasawara ◽

Hiroyuki Takeda ◽

Tatsuya Sawasaki ◽

Yoshio Ikeda ◽

...

Keyword(s):

Protein Synthesis ◽

Ligand Binding ◽

Germ Cell ◽

Wheat Germ ◽

D1 Receptors ◽

Synthesis System ◽

Binding Ability ◽

Free Protein ◽

Protein Synthesis System ◽

Cell Free Protein Synthesis

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