Research highlights: cell separation at the bench and beyond

Lab on a Chip ◽  
2015 ◽  
Vol 15 (3) ◽  
pp. 605-609 ◽  
Author(s):  
Anja Kunze ◽  
James Che ◽  
Armin Karimi ◽  
Dino Di Carlo
Keyword(s):  
Cell Separation ◽  
Cell Filtration ◽  
Combined Solution ◽  
Pipette Tip ◽  
Solution Transfer

Microfluidic pipette tip for combined solution transfer and cell filtration – Physical separations for improved cell-based therapies – Separating cells from the living brain with nanomagnets.

Clog-free cell filtration using resettable cell traps

Lab on a Chip ◽  
2014 ◽  
Vol 14 (15) ◽  
pp. 2657-2665 ◽  
Author(s):  
William Beattie ◽  
Xi Qin ◽  
Lin Wang ◽  
Hongshen Ma
Keyword(s):  
High Throughput ◽  
Cell Separation ◽  
Free Cell ◽  
Separation Mechanism ◽  
Cell Filtration ◽  
Capture And Release

A microfluidic cell separation mechanism created using constrictions with adjustable size that can selectively capture and release cells, thereby enabling high throughput size and deformability based cell separation without clogging.

Anatomy and ultrastructure of haustoria in selected West Australian parasitic angiosperms

1990 ◽  
Vol 48 (3) ◽  
pp. 690-691
Author(s):  
John Kuo ◽  
John S. Pate
Keyword(s):  
Parasitic Plants ◽  
The Other ◽  
Nutrient Transfer ◽  
Direct Solution ◽  
Tracheary Elements ◽  
Solute Transfer ◽  
Glycol Methacrylate ◽  
Australian Species ◽  
Anatomy And Ultrastructure ◽  
Solution Transfer

Our understanding of nutrient transfer between host and flowering parasitic plants is usually based mainly on physiological concepts, with little information on haustorial structure related to function. The aim of this paper is to study the haustorial interface and possible pathways of water and solute transfer between a number of host and parasites.Haustorial tissues were fixed in glutaraldehyde and embedded in glycol methacrylate (LM), or fixed in glutaraldehyde then OsO4 and embedded in Spurr’s resin (TEM).Our study shows that lumen to lumen continuity occurs between tracheary elements of a host and four S.W. Australian species of aerial mistletoes (Fig. 1), and some root hemiparasites (Exocarpos spp. and Anthobolus foveolatus) (Fig. 2). On the other hand, haustorial interfaces of the root hemiparasites Olax phyllanthi and Santalum (2 species) are comprised mainly of parenchyma, as opposed to terminating tracheads or vessels, implying that direct solution transfer between partners via vessels or tracheary elements may be limited (Fig. 3).

Development and study of a method for cell separation during white blood cell segmentation on images of bone marrow preparations in information and measurement systems for diagnostics of acute leukemia

2020 ◽  
pp. 68-72
Author(s):  
V.G. Nikitaev ◽  
A.N. Pronichev ◽  
V.V. Dmitrieva ◽  
E.V. Polyakov ◽  
A.D. Samsonova ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Cell ◽  
Acute Leukemia ◽  
White Blood Cell ◽  
Cell Separation ◽  
Large Scale ◽  
Bone Marrow Cells ◽  
Automated Analysis ◽  
Cell Segmentation ◽  
Measurement Systems

The issues of using of information and measurement systems based on processing of digital images of microscopic preparations for solving large-scale tasks of automating the diagnosis of acute leukemia are considered. The high density of leukocyte cells in the preparation (hypercellularity) is a feature of microscopic images of bone marrow preparations. It causes the proximity of cells to eachother and their contact with the formation of conglomerates. Measuring of the characteristics of bone marrow cells in such conditions leads to unacceptable errors (more than 50%). The work is devoted to segmentation of contiguous cells in images of bone marrow preparations. A method of cell separation during white blood cell segmentation on images of bone marrow preparations under conditions of hypercellularity of the preparation has been developed. The peculiarity of the proposed method is the use of an approach to segmentation of cell images based on the watershed method with markers. Key stages of the method: the formation of initial markers and builds the lines of watershed, a threshold binarization, shading inside the outline. The parameters of the separation of contiguous cells are determined. The experiment confirmed the effectiveness of the proposed method. The relative segmentation error was 5 %. The use of the proposed method in information and measurement systems of computer microscopy for automated analysis of bone marrow preparations will help to improve the accuracy of diagnosis of acute leukemia.

Clinical Labeling and Imaging of Transplanted CD133+/CD34+ Stem Cells in Patients with Ischemic Heart Disease

2012 ◽  
Vol 15 (2) ◽  
pp. 116 ◽  
Author(s):  
Ali Ghodsizad ◽  
Viktor Bordel ◽  
Brian Bruckner ◽  
Mathias Loebe ◽  
Gunter Fuerst ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Separation ◽  
Coronary Artery Bypass Surgery ◽  
Iliac Crest ◽  
Artery Bypass ◽  
Good Manufacturing Practice ◽  
Novel Method ◽  
End Stage Heart Failure ◽  
End Stage

The application of somatic stem cells has been shown to support the recovery of the myocardium in end-stage heart failure. A novel method for the intraoperative isolation and labeling of bone marrow-derived stem cells was established. After induction of general anesthesia, up to 400 mL of bone marrow were harvested from the posterior iliac crest and processed in the operating room under good manufacturing practice conditions by means of the automated cell-selection device Clini-MACS (Miltenyi Biotec). We subsequently injected autologous CD133<sup>+</sup> and CD34<sup>+</sup> stem cells in a predefined pattern around the laser channels in patients undergoing coronary artery bypass surgery and transmyocardial laser procedures. Intraoperative isolation and labeling is an effective cell-separation tool for the future, considering that novel cell markers can be promising new candidates for cell therapy.

Blood Cell Separation Device Using Serially Connected Membrane Filters for Adapting to Blood Flow Properties

2009 ◽  
Vol 129 (11) ◽  
pp. 380-386 ◽  
Author(s):  
Taizo Kobayashi ◽  
Daiki Kato ◽  
Hiroyuki Koga ◽  
Kenichi Morimoto ◽  
Makoto Fukuda ◽  
...  
Keyword(s):  
Blood Flow ◽  
Blood Cell ◽  
Cell Separation ◽  
Flow Properties ◽  
Separation Device ◽  
Membrane Filters
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