scholarly journals Preparation and mechanical characterisation of giant unilamellar vesicles by a microfluidic method

Lab on a Chip ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 557-562 ◽  
Author(s):  
K. Karamdad ◽  
R. V. Law ◽  
J. M. Seddon ◽  
N. J. Brooks ◽  
O. Ces

We present a novel method for the generation of giant unilamellar vesicles and the use of fluctuation analysis to characterise their mechanical properties.

2019 ◽  
Vol 553 ◽  
pp. 247-258 ◽  
Author(s):  
Andrea Mescola ◽  
Nathaly Marín-Medina ◽  
Gregorio Ragazzini ◽  
Maurizio Accolla ◽  
Andrea Alessandrini

2016 ◽  
Vol 52 (30) ◽  
pp. 5277-5280 ◽  
Author(s):  
K. Karamdad ◽  
R. V. Law ◽  
J. M. Seddon ◽  
N. J. Brooks ◽  
O. Ces

In this article we detail a robust high-throughput microfluidic platform capable of fabricating either symmetric or asymmetric giant unilamellar vesicles (GUVs) and characterise the mechanical properties of their membranes.


Author(s):  
Tao Gu ◽  
Shuaixin Zhang ◽  
Yuhong Zhao ◽  
Yurong Yang ◽  
Huafeng Liu ◽  
...  

Life ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 634
Author(s):  
Ylenia Miele ◽  
Gábor Holló ◽  
István Lagzi ◽  
Federico Rossi

The budding and division of artificial cells engineered from vesicles and droplets have gained much attention in the past few decades due to an increased interest in designing stimuli-responsive synthetic systems. Proper control of the division process is one of the main challenges in the field of synthetic biology and, especially in the context of the origin of life studies, it would be helpful to look for the simplest chemical and physical processes likely at play in prebiotic conditions. Here we show that pH-sensitive giant unilamellar vesicles composed of mixed phospholipid/fatty acid membranes undergo a budding process, internally fuelled by the urea–urease enzymatic reaction, only for a given range of the membrane composition. A gentle interplay between the effects of the membrane composition on the elasticity and the preferred area difference of the bilayer is responsible for the existence of a narrow range of membrane composition yielding a high probability for budding of the vesicles.


Langmuir ◽  
2021 ◽  
Vol 37 (3) ◽  
pp. 1082-1088
Author(s):  
Chiho Kataoka-Hamai ◽  
Kohsaku Kawakami

Soft Matter ◽  
2021 ◽  
Author(s):  
Peng Bao ◽  
Daniel A. Paterson ◽  
Sally A. Peyman ◽  
J. Cliff Jones ◽  
Jonathan A. T. Sandoe ◽  
...  

We describe a modified microfluidic method for making Giant Unilamellar Vesicles (GUVs) via water/octanol-lipid/water double emulsion droplets and encapsulation of nematic lyotropic liquid crystals (LNLCs).


2021 ◽  
Vol 120 (3) ◽  
pp. 147a
Author(s):  
Thais A. Enoki ◽  
Haden L. Scott ◽  
Gerald W. Feigenson ◽  
Frederick A. Heberle

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