scholarly journals Technical bias of microcultivation environments on single-cell physiology

Lab on a Chip ◽  
2015 ◽  
Vol 15 (8) ◽  
pp. 1822-1834 ◽  
Author(s):  
Christian Dusny ◽  
Alexander Grünberger ◽  
Christopher Probst ◽  
Wolfgang Wiechert ◽  
Dietrich Kohlheyer ◽  
...  
Keyword(s):  
Growth Rate ◽  
Single Cell ◽  
Specific Growth ◽  
Cell Physiology ◽  
Cell Cultivation ◽  
Division Rate ◽  
Platform Comparison ◽  
Cross Platform ◽  
Technical Bias ◽  
Single Cell Physiology

The cross-platform comparison of three different single-cell cultivation methods demonstrates technical influences on biological key parameters like specific growth rate, division rate and cellular morphology.

scholarly journals Using measures of single‐cell physiology and physiological state to understand organismic aging

Aging Cell ◽  
2015 ◽  
Vol 15 (1) ◽  
pp. 4-13 ◽  
Author(s):  
Alexander Mendenhall ◽  
Monica Driscoll ◽  
Roger Brent
Keyword(s):  
Single Cell ◽  
Physiological State ◽  
Cell Physiology ◽  
Single Cell Physiology

Single Cell Physiology

2009 ◽  
pp. 305-316 ◽  
Author(s):  
Pierre Neveu ◽  
Deepak Kumar Sinha ◽  
Petronella Kettunen ◽  
Sophie Vriz ◽  
Ludovic Jullien ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Physiology ◽  
Single Cell Physiology

scholarly journals SINC-seq: correlation of transient gene expressions between nucleus and cytoplasm reflects single-cell physiology

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Mahmoud N. Abdelmoez ◽  
Kei Iida ◽  
Yusuke Oguchi ◽  
Hidekazu Nishikii ◽  
Ryuji Yokokawa ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Physiology ◽  
Gene Expressions ◽  
Single Cell Physiology

Effective dose of a microbial inoculant is one to four cells in the rhizosphere

2002 ◽  
Vol 48 (10) ◽  
pp. 940-944 ◽  
Author(s):  
Bo Normander ◽  
Niels Bohse Hendriksen
Keyword(s):  
Risk Assessment ◽  
Biological Control ◽  
Growth Rate ◽  
Single Cell ◽  
Pseudomonas Fluorescens ◽  
Specific Growth ◽  
Growth Potential ◽  
Bacterial Cells ◽  
Population Sizes ◽  
Number Of Bacteria

A single-cell approach for studying the growth potential and the establishment of bacteria in the barley phytosphere is presented, using Pseudomonas fluorescens strain with the capability for biological control. The incidence of growth of one to four bacterial cells dispersed to the young rhizosphere approximated to 100%, and specific growth rate averaged 0.05. Net growth occurred for cells added to the rhizosphere at densities between 1 and 100 000 cells, while at higher densities population sizes declined, but always approached 105–106cells per rhizosphere. No net growth was observed in bulk soil, and cells died in the phyllosphere. Our results showed that bacterial establishment was more related to the availability of microhabitats supporting growth, than related to the number of bacteria released. Key words: risk assessment, bacterial fate, single-cell, Pseudomonas fluorescens, gfp.

scholarly journals Single-Cell Physiology

2015 ◽  
Vol 44 (1) ◽  
pp. 123-142 ◽  
Author(s):  
Sattar Taheri-Araghi ◽  
Steven D. Brown ◽  
John T. Sauls ◽  
Dustin B. McIntosh ◽  
Suckjoon Jun
Keyword(s):  
Single Cell ◽  
Cell Physiology ◽  
Single Cell Physiology

scholarly journals dMSCC: A microfluidic platform for microbial single-cell cultivation under dynamic environmental medium conditions

2020 ◽  
Author(s):  
Sarah Täuber ◽  
Corinna Golze ◽  
Phuong Ho ◽  
Eric von Lieres ◽  
Alexander Grünberger
Keyword(s):  
Growth Rate ◽  
Single Cell ◽  
Environmental Conditions ◽  
Cellular Response ◽  
Large Scale ◽  
Single Cells ◽  
Model Organism ◽  
Colony Growth ◽  
Cell Cultivation ◽  
Natural Habitats

AbstractIn nature and in technical systems, microbial cells are often exposed to rapidly fluctuating environmental conditions. These conditions can vary in quality, e.g., existence of a starvation zone, and quantity, e.g., average residence time in this zone. For strain development and process design, cellular response to such fluctuations needs to be systematically analysed. However, the existing methods for physically emulating rapidly changing environmental conditions are limited in spatio-temporal resolution. Hence, we present a novel microfluidic system for cultivation of single cells and small cell clusters under dynamic environmental conditions (dynamic microfluidic single-cell cultivation (dMSCC)). This system enables to control nutrient availability and composition between two media with second to minute resolution. We validate our technology using the industrially relevant model organism Corynebacterium glutamicum. The organism was exposed to different oscillation frequencies between nutrient excess (feasts) and scarcity (famine). Resulting changes in cellular physiology, such as the colony growth rate and cell morphology were analysed and revealed significant differences with growth rate and cell length between the different conditions. dMSCC also allows to apply defined but randomly changing nutrient conditions, which is important for reproducing more complex conditions from natural habitats and large-scale bioreactors. The presented system lays the foundation for the cultivation of cells under complex changing environmental conditions.

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