Three-dimensional brain-on-a-chip with an interstitial level of flow and its application as an in vitro model of Alzheimer's disease

Lab on a Chip ◽  
2015 ◽  
Vol 15 (1) ◽  
pp. 141-150 ◽  
Author(s):  
JiSoo Park ◽  
Bo Kyeong Lee ◽  
Gi Seok Jeong ◽  
Jung Keun Hyun ◽  
C. Justin Lee ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Interstitial Flow ◽  
Microwell Array ◽  
Model Of Alzheimer’S Disease ◽  
Vitro Model

In this paper, we developed a three-dimensional brain-on-a-chip with an interstitial level of flow. The chip contains an osmotic micropump system for providing interstitial flow and a concave microwell array for mimicking the brain's 3D cytoarchitecture.

scholarly journals The Moringin/α-CD Pretreatment Induces Neuroprotection in an In Vitro Model of Alzheimer’s Disease: A Transcriptomic Study

2021 ◽  
Vol 43 (1) ◽  
pp. 197-214
Author(s):  
Serena Silvestro ◽  
Luigi Chiricosta ◽  
Agnese Gugliandolo ◽  
Renato Iori ◽  
Patrick Rollin ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
In Vitro Model ◽  
Neurodegenerative Disorder ◽  
Senile Dementia ◽  
Neuroprotective Effects ◽  
Model Of Alzheimer’S Disease ◽  
Vitro Model ◽  
Ad Prevention

Alzheimer’s disease (AD) is a progressive neurodegenerative disorder and represents the most common form of senile dementia. Autophagy and mitophagy are cellular processes that play a key role in the aggregation of β-amyloid (Aβ) and tau phosphorylation. As a consequence, impairment of these processes leads to the progression of AD. Thus, interest is growing in the search for new natural compounds, such as Moringin (MOR), with neuroprotective, anti-amyloidogenic, antioxidative, and anti-inflammatory properties that could be used for AD prevention. However, MOR appears to be poorly soluble and stable in water. To increase its solubility MOR was conjugated with α-cyclodextrin (MOR/α-CD). In this work, it was evaluated if MOR/α-CD pretreatment was able to exert neuroprotective effects in an AD in vitro model through the evaluation of the transcriptional profile by next-generation sequencing (NGS). To induce the AD model, retinoic acid-differentiated SH-SY5Y cells were exposed to Aβ1-42. The MOR/α-CD pretreatment reduced the expression of the genes which encode proteins involved in senescence, autophagy, and mitophagy processes. Additionally, MOR/α-CD was able to induce neuronal remodeling modulating the axon guidance, principally downregulating the Slit/Robo signaling pathway. Noteworthy, MOR/α-CD, modulating these important pathways, may induce neuronal protection against Aβ1-42 toxicity as demonstrated also by the reduction of cleaved caspase 3. These data indicated that MOR/α-CD could attenuate the progression of the disease and promote neuronal repair.

Neuroprotective Effects of Statins in an In Vitro Model of Alzheimer's Disease

2009 ◽  
Vol 17 (3) ◽  
pp. 503-517 ◽  
Author(s):  
Ana Catarina R.G. Fonseca ◽  
Teresa Proença ◽  
Rosa Resende ◽  
Catarina R. Oliveira ◽  
Cláudia M.F. Pereira
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
In Vitro Model ◽  
Neuroprotective Effects ◽  
Model Of Alzheimer’S Disease ◽  
Vitro Model

Dual Effect of Doxazosin: Anticancer Activity on SH-SY5Y Neuroblastoma Cells and Neuroprotection on an In Vitro Model of Alzheimer's Disease

Neuroscience ◽  
2019 ◽  
Vol 404 ◽  
pp. 314-325 ◽  
Author(s):  
Bárbara Paranhos Coelho ◽  
Mariana Maier Gaelzer ◽  
Fernanda dos Santos Petry ◽  
Juliana Bender Hoppe ◽  
Vera Maria Treis Trindade ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Anticancer Activity ◽  
In Vitro Model ◽  
Neuroblastoma Cells ◽  
Dual Effect ◽  
Model Of Alzheimer’S Disease ◽  
Vitro Model
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