scholarly journals First steps towards a generic sample preparation scheme for inorganic engineered nanoparticles in a complex matrix for detection, characterization, and quantification by asymmetric flow-field flow fractionation coupled to multi-angle light scattering and ICP-MS

2015 ◽  
Vol 30 (6) ◽  
pp. 1286-1296 ◽  
Author(s):  
S. Wagner ◽  
S. Legros ◽  
K. Loeschner ◽  
J. Liu ◽  
J. Navratilova ◽  
...  
Keyword(s):  
Light Scattering ◽  
Sample Preparation ◽  
Flow Field ◽  
Engineered Nanoparticles ◽  
Field Flow Fractionation ◽  
Complex Matrix ◽  
Asymmetric Flow ◽  
Complex Matrices ◽  
Icp Ms ◽  
Flow Fractionation

Engineering nanoparticles in complex matrices.

scholarly journals Limitations of Nanoparticles Size Characterization by Asymmetric Flow Field‑Fractionation Coupled with Online Dynamic Light Scattering

2021 ◽  
Author(s):  
Francesco Giorgi ◽  
Judith M. Curran ◽  
Douglas Gilliland ◽  
Rita La Spina ◽  
Maurice Whelan ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Flow Field ◽  
Field Flow Fractionation ◽  
Asymmetric Flow ◽  
Uv Detector ◽  
Elution Time ◽  
On Line ◽  
Real Size ◽  
Flow Fractionation

AbstractThe development of reliable protocols suitable for the characterisation of the physical properties of nanoparticles in suspension is becoming crucial to assess the potential biological as well as toxicological impact of nanoparticles. Amongst sizing techniques, asymmetric flow field flow fractionation (AF4) coupled to online size detectors represents one of the most robust and flexible options to quantify the particle size distribution in suspension. However, size measurement uncertainties have been reported for on-line dynamic light scattering (DLS) detectors when coupled to AF4 systems. In this work we investigated the influence of the initial concentration of nanoparticles in suspension on the sizing capability of the asymmetric flow field-flow fractionation technique coupled with an on-line dynamic light scattering detector and a UV–Visible spectrophotometer (UV) detector. Experiments were performed with suspensions of gold nanoparticles with a nominal diameter of 40 nm and 60 nm at a range of particle concentrations. The results obtained demonstrate that at low concentration of nanoparticles, the AF4-DLS combined technique fails to evaluate the real size of nanoparticles in suspension, detecting an apparent and progressive size increase as a function of the elution time and of the concentration of nanoparticles in suspension.

scholarly journals Challenges in Determining the Size Distribution of Nanoparticles in Consumer Products by Asymmetric Flow Field-Flow Fractionation Coupled to Inductively Coupled Plasma-Mass Spectrometry: The Example of Al2O3, TiO2, and SiO2 Nanoparticles in Toothpaste

Separations ◽  
2018 ◽  
Vol 5 (4) ◽  
pp. 56 ◽  
Author(s):  
Manuel Correia ◽  
Toni Uusimäki ◽  
Allan Philippe ◽  
Katrin Loeschner
Keyword(s):  
Mass Spectrometry ◽  
Particle Size ◽  
Flow Field ◽  
Inductively Coupled Plasma ◽  
Field Flow Fractionation ◽  
Asymmetric Flow ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Flow Fractionation

According to the current European regulation on cosmetics, any ingredient present as a nanomaterial should be indicated in the ingredient list. There is a need for analytical methods capable of determining the size of the relevant ingredients and thus assessing if these are nanomaterials or not. An analytical method based on asymmetric flow field-flow fractionation (AF4) and inductively coupled plasma-mass spectrometry (ICP-MS) was developed to determine the size of particles present in a commercial toothpaste. Multi-angle light scattering (MALS) was used for on-line size determination. The number-based particle size distributions (PSDs) of the particles were retrieved upon mathematical conversion of the mass-based PSDs recovered from the AF4-ICP-MS fractograms. AF4-ICP-MS allowed to separate and detect Al2O3 and TiO2 particles in the toothpaste and to retrieve a correct TiO2 number-based PSD. The potential presence of particles in the lower size range of the Al2O3 mass-based PSD had a strong impact on sizing and nanomaterial classification upon conversion. AF4 coupled with ICP-MS and MALS was found to be a powerful approach for characterization of different particles in a multiple-particle system such as toothpaste. Confirmation of particle size by a secondary method such as single particle ICP-MS or hydrodynamic chromatography was crucial.

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