Review of the applications of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to the analysis of biological samples

2014 ◽  
Vol 29 (12) ◽  
pp. 2204-2228 ◽  
Author(s):  
Dirce Pozebon ◽  
Guilherme L. Scheffler ◽  
Valderi L. Dressler ◽  
Matheus A. G. Nunes
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Biological Samples ◽  
Inductively Coupled Plasma ◽  
Life Sciences ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Number Of Publications

Laser ablation inductively coupled plasma mass spectrometry has been proven to be useful in life sciences as can be observed by the increasing number of publications in this field.

Evaluation of a custom single Peltier-cooled ablation cell for elemental imaging of biological samples in laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS)

2016 ◽  
Vol 31 (4) ◽  
pp. 1030-1033 ◽  
Author(s):  
J. S. Hamilton ◽  
E. L. Gorishek ◽  
P. M. Mach ◽  
D. Sturtevant ◽  
M. L. Ladage ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Biological Samples ◽  
Inductively Coupled Plasma ◽  
Peltier Element ◽  
Elemental Imaging ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Biological Sampling

A new single Peltier element ablation cell is described and its applicability to biological sampling discussed to evaluate its performance.

Imaging of metals and metal-containing species in biological tissues and on gels by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS): A new analytical strategy for applications in life sciences

2008 ◽  
Vol 80 (12) ◽  
pp. 2643-2655 ◽  
Author(s):  
J. Sabine Becker ◽  
M. Zoriy ◽  
Valderi L. Dressler ◽  
Bei Wu ◽  
J. Susanne Becker
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Gel Electrophoresis ◽  
Inductively Coupled Plasma ◽  
Toxic Elements ◽  
Life Sciences ◽  
Biological Tissues ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has become established as a very efficient and sensitive trace, ultratrace, and surface analytical technique in the life sciences. We have developed a new analytical imaging technique using LA-ICP-MS to study element distribution in biological tissues. Nowadays, LA imaging ICP-MS using double-focusing sector field (LA-ICP-SFMS) or quadrupole-based mass spectrometers (LA-ICP-QMS) can be applied as an exciting tool providing new information on the pathophysiology, pharmacology, and toxicology of elements of interest in biological systems. The quantitative determination of elements (e.g., Cu, Fe, Zn, Se, and others) in biological tissues is of growing interest especially in brain research (e.g., for studying neurodegenerative diseases such as Alzheimer's or Parkinson's disease). LA-ICP-SFMS was employed to produce images of detailed regionally specific element distributions in thin tissue sections of different sizes (such as control human or rat brain tissues or tumor regions). In addition, imaging MS using LA-ICP-QMS was applied to study the uptake and transport of nutrient and toxic elements in plant tissues.Besides the quantitative imaging of essential and toxic elements in tissues, powerful analytical techniques are also required for the determination and characterization of phosphoproteins and metal-containing proteins within a large pool of proteins, after electrophoretic separation (e.g., blue native, BN and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, SDS-PAGE) into 1D and 2D gels. LA-ICP-MS was used to detect metalloproteins in protein bands of 1D gels or protein spots separated after 2D gel electrophoresis (2D-GE). In addition to elemental determination by LA-ICP-MS, matrix-assisted laser desorption/ionization (MALDI)-MS was employed to identify metal-containing proteins. Recent progress will be discussed in applying LA-ICP-MS in the life sciences, including the imaging of thin slices of tissue and applications in proteome analysis in combination with MALDI-MS to investigate phosphoproteins and metal-containing proteins.

Nanoparticles Determination by Laser Ablation Inductively Coupled Plasma Mass Spectrometry

2021 ◽  
Vol 21 (11) ◽  
pp. 5436-5442
Author(s):  
Wenhe Luo ◽  
Tao Li ◽  
Meng Wang ◽  
Wanqin Dai ◽  
Chunlei Jiao ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrum ◽  
Laser Ablation ◽  
Biological Samples ◽  
Inductively Coupled Plasma ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Dissolved Ions ◽  
Plasma Mass Spectrometry ◽  
Signal Profile

Quantitatively studying the biodistribution and transformation of nanomaterials is of great importance for nanotoxicological evaluation. Recently, laser ablation inductively coupled plasma mass spectrometry has been employed to distinguish nanoparticles (NPs) with their dissolved ions in biological samples. The principle of the proposal is based on a hypothesis that the intact NPs sampled by laser ablation will generate discrete sharp pulses of signals in ICP-MS measurement, being totally different from the continuous, relatively lower signals generated by ions. However, it is still a controversy whether NPs could maintain their intactness during the laser ablation. This work found a way to exactly determine the number of NPs sampled for each LA-ICP-MS measurement. It made possible to reveal the signal profile of a single NP in LA-ICP-MS analysis. The results suggest that AuNR, AgNP and TIO2 NP were broken into much smaller secondary NPs during the laser ablation, therefore generating continuous signals in the analyzer. There was a certain probability that the fragmentation of large-sized NP or multiple NPs by laser ablation was not sufficient, leaving some NPs unbroken or some secondary NPs with relatively large sizes to generate discrete pulses of signals in the analyzer. When the intactness of NPs during laser ablation cannot be assured, it is impossible to determine the attribution of mass spectrum signals. These findings compromise the reliability of distinguishing NPs from their dissolved ions by LA-ICP-MS.

Laser ablation inductively coupled plasma mass spectrometry for multi-elemental determination in dried blood spots

2017 ◽  
Vol 32 (8) ◽  
pp. 1500-1507 ◽  
Author(s):  
Jorge Moreda-Piñeiro ◽  
Alicia Cantarero-Roldán ◽  
Antonio Moreda-Piñeiro ◽  
José Ángel Cocho ◽  
Pilar Bermejo-Barrera
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Inductively Coupled Plasma ◽  
Dried Blood Spots ◽  
Element Determination ◽  
Inductively Coupled ◽  
Inductively Coupled Mass Spectrometry ◽  
Icp Ms ◽  
Elemental Determination ◽  
Plasma Mass Spectrometry

An approach by laser ablation (LA) coupled with inductively coupled mass spectrometry (ICP-MS) for multi-element determination in whole blood is described.

Micrometer-Resolved Binding of Lead to Iron in Urban River Sediments

2004 ◽  
Vol 57 (10) ◽  
pp. 921 ◽  
Author(s):  
Sebastien Rauch ◽  
Gregory M. Morrison
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Inductively Coupled Plasma ◽  
River Sediments ◽  
Element Analysis ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Recent Developments ◽  
Plasma Mass Spectrometry ◽  
Multi Element Analysis

Recent developments in laser microprobes and the coupling of laser ablation (LA) to inductively coupled plasma mass spectrometry (ICP-MS) has opened up the possibility for detailed analysis of individual sediment grains and the elucidation of metal association with bulk elements and phases. Here, LA–ICP-MS is used for the micrometer-resolved, multi-element analysis of Pb and bulk elements such as Fe, Mg, Al, and Si. Normalized Pb signals show a clear association to Fe coatings at the surface of the particles.

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