Elemental and molecular mass spectrometry for integrated selenosugar speciation in liver and kidney tissues of maternal feeding and supplemented rats

2015 ◽  
Vol 30 (1) ◽  
pp. 267-276 ◽  
Author(s):  
Héctor González-Iglesias ◽  
María Luisa Fernández-Sánchez ◽  
Ying Lu ◽  
Sonia Fernández Menéndez ◽  
Spiros A. Pergantis ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Mass ◽  
Rat Liver ◽  
Ion Sources ◽  
Selenium Speciation ◽  
Molecular Ion ◽  
Molecular Mass Spectrometry ◽  
Liver And Kidney

The integrated use of elemental and molecular ion sources for enhanced selenium speciation data in rat liver and kidneys, along with its potential for Se-supplement studies are highlighted.

scholarly journals Properties of subunits of the multicatalytic proteinase complex revealed by the use of subunit-specific antibodies

1991 ◽  
Vol 278 (1) ◽  
pp. 171-177 ◽  
Author(s):  
A J Rivett ◽  
S T Sweeney
Keyword(s):  
Molecular Mass ◽  
Rat Liver ◽  
Gel Filtration ◽  
Immunoblot Analysis ◽  
Rat Tissues ◽  
Specific Antibodies ◽  
Multicatalytic Proteinase ◽  
Cell Extracts ◽  
Liver And Kidney ◽  
Lysosomal Proteinase

The multicatalytic proteinase (MCP) is a high-molecular-mass non-lysosomal proteinase that gives rise to a characteristic pattern of bands of molecular mass 22-34 kDa on SDS/PAGE gels. Isoelectric-focusing gels of the enzyme purified from rat liver show 16 bands with isoelectric points in the range of pH 5-8.5. Two-dimensional PAGE gels reveal that there are more than the previously reported 13 polypeptides associated with the MCP from rat liver and show a pattern of 15-20 major spots and several minor ones, similar to that of MCP isolated from some other sources. Possible relationships between the different polypeptides were investigated by immunoblot analysis of electrophoretically purified proteinase subunits with affinity-purified subunit-specific antibodies as well as antibodies raised against individual denatured subunits of the complex. The results demonstrate that many of the major polypeptide components of the MCP complex are antigenically distinct. Moreover comparison of immunoreactive material in crude cell extracts with that in purified MCP preparations has shown that the polypeptides are not derived from a smaller number of higher-molecular-mass subunits. Also, individual subunits have the same apparent molecular mass in a variety of rat tissues, suggesting close similarity between MCPs of different tissues. The highest concentrations of MCP subunits occur in liver and kidney. Gel-filtration analysis of crude extracts has demonstrated that MCP polypeptides are also associated with a higher-molecular-mass complex, which may be the 26 S proteinase that has been implicated in the degradation of ubiquitin-protein conjugates.

Use of molecular mass spectrometry in studying composition of dispersion media in lubricating greases

1978 ◽  
Vol 14 (9) ◽  
pp. 699-702
Author(s):  
L. N. Sosulina ◽  
L. I. Nazarova ◽  
E. M. Nikonorov ◽  
T. G. Zubkevich
Keyword(s):  
Mass Spectrometry ◽  
Molecular Mass ◽  
Molecular Mass Spectrometry ◽  
Dispersion Media

Use of elemental and molecular-mass spectrometry to assess the toxicological effects of inorganic mercury in the mouse Mus musculus

2014 ◽  
Vol 406 (24) ◽  
pp. 5853-5865 ◽  
Author(s):  
Miguel Ángel García-Sevillano ◽  
Tamara García-Barrera ◽  
Francisco Navarro ◽  
Jürgen Gailer ◽  
José Luiz Gómez-Ariza
Keyword(s):  
Mass Spectrometry ◽  
Molecular Mass ◽  
Mus Musculus ◽  
Inorganic Mercury ◽  
Toxicological Effects ◽  
Molecular Mass Spectrometry

Atomic (HPLC-ICP-MS) and molecular mass spectrometry (ESI-Q-TOF) to study cis-platin interactions with serum proteins

2008 ◽  
Vol 23 (3) ◽  
pp. 378-384 ◽  
Author(s):  
Diego Esteban-Fernández ◽  
M. Montes-Bayón ◽  
E. Blanco González ◽  
M. M. Gómez Gómez ◽  
M. A. Palacios ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Mass ◽  
Serum Proteins ◽  
Icp Ms ◽  
Molecular Mass Spectrometry

Imaging by Elemental and Molecular Mass Spectrometry Reveals the Uptake of an Arsenolipid in the Brain of Drosophila melanogaster

2016 ◽  
Vol 88 (10) ◽  
pp. 5258-5263 ◽  
Author(s):  
Ann-Christin Niehoff ◽  
Jacqueline Schulz ◽  
Jens Soltwisch ◽  
Sören Meyer ◽  
Hans Kettling ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drosophila Melanogaster ◽  
Molecular Mass ◽  
Molecular Mass Spectrometry ◽  
The Brain
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