Sequence-specific recognition of single-stranded DNA using atomic absorption spectrometry

2014 ◽  
Vol 29 (9) ◽  
pp. 1591-1597 ◽  
Author(s):  
Hong Zhang ◽  
Zhifang Zhu ◽  
Zunxiang Zeng ◽  
Liansheng Ling
Keyword(s):  
Gold Nanoparticles ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Absorption Spectrometry ◽  
Single Stranded Dna ◽  
Coated Glass ◽  
Specific Recognition

A technique for the sequence-specific recognition of single-stranded DNA using gold nanoparticles and silver-coated glass modified with oligonucleotides was developed, with determination by atomic absorption spectrometry.

scholarly journals Atomic Absorption Spectrometry as an Alternative to Determine the Presence of Gold Nanoparticles on or in Silica Matrix

2022 ◽  
Author(s):  
Cristian Giertyas ◽  
Victor Silva ◽  
Maria de Oliveira ◽  
Emerson Freire ◽  
Josué Santos ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Absorption Spectrometry ◽  
Silica Matrix ◽  
Emission Spectrometry ◽  
Sample Treatment ◽  
Plasma Atomic Emission Spectrometry ◽  
Flame Atomic Absorption ◽  
Vis Spectroscopy

Two different gold-silica-based nanomaterials were prepared: (i) silica-supported gold nanoparticles (AuNP/SiO2); and (ii) gold-silica core-shell nanoparticles (AuNP@SiO2). Three strategies for sample treatment (S), consisting in acid treatments, were employed: (S1) HNO3; (S2) HNO3 + HCl; and (S3) HF + HNO3 + HCl, applying microwave oven digestion for S2 and S3. From three calibration curves, slope, intercept, and linear correlation coefficient were obtained. The accuracy of the methods was evaluated by comparing the gold contents in a sample determined by flame atomic absorption spectrometry (FAAS) and by inductively coupled plasma atomic emission spectrometry (ICP-OES). Finally, the amount of gold for all samples was determined by FAAS. UV-Vis spectroscopy and transmission electron microscopy (TEM) were used to compare the material before and after sample treatment. By comparison, the application of S2 and S3 allowed the presence of gold on or in the silica matrix to be determined and the amount quantified.

scholarly journals Analytical Performance and Validation of a Reliable Method Based on Graphite Furnace Atomic Absorption Spectrometry for the Determination of Gold Nanoparticles in Biological Tissues

Nanomaterials ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3370
Author(s):  
Oana Cadar ◽  
Teodora Mocan ◽  
Cecilia Roman ◽  
Marin Senila
Keyword(s):  
Gold Nanoparticles ◽  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Limit Of Detection ◽  
Tissue Sample ◽  
Graphite Furnace ◽  
Absorption Spectrometry ◽  
Biological Tissues ◽  
Graphite Furnace Atomic Absorption

Gold nanoparticles (AuNPs) have a wide-ranging application and are widespread in samples with complex matrices; thus, efficient analytical procedures are necessary to identify and characterize this analyte. A sensitive analytical method for determination of AuNPs content in biological tissues, based on microwave-assisted acid wet digestion and graphite furnace atomic absorption spectrometry (GFAAS) validated in accordance with the requirements of Eurachem guideline and ISO 17025 standard, is presented in this study. The digestion procedure was optimized, and the figures of merit such as selectivity, limit of detection (0.43 µg L−1), limit of quantification (1.29 µg L−1, corresponding to 12.9 µg kg−1 in tissue sample, considering the digestion), working range, linearity, repeatability ((RSDr 4.15%), intermediate precision (RSDR 8.07%), recovery in accuracy study (97%), were methodically evaluated. The measurement uncertainty was assessed considering the main sources of uncertainties and the calculated relative expanded uncertainty (k = 2) was 12.5%. The method was applied for the determination of AuNPs in six biological tissues (liver, small intestine, heart, lungs, brain and kidneys) and the found concentrations were generally at low levels, close or lower than LOQ.

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