In vitro bioactive properties of intact and enzymatically hydrolysed whey protein: targeting the enteroinsular axis

O. Power-Grant; C. Bruen; L. Brennan; L. Giblin; P. Jakeman; R. J. FitzGerald

doi:10.1039/c4fo00983e

In vitro bioactive properties of intact and enzymatically hydrolysed whey protein: targeting the enteroinsular axis

Food & Function ◽

10.1039/c4fo00983e ◽

2015 ◽

Vol 6 (3) ◽

pp. 972-980 ◽

Cited By ~ 26

Author(s):

O. Power-Grant ◽

C. Bruen ◽

L. Brennan ◽

L. Giblin ◽

P. Jakeman ◽

...

Keyword(s):

Whey Protein ◽

Protein Targeting ◽

Dipeptidyl Peptidase ◽

Protein Hydrolysates ◽

Dipeptidyl Peptidase Iv ◽

Enteroinsular Axis ◽

Whey Protein Hydrolysates ◽

Bioactive Properties

This work identified extensively hydrolysed whey protein hydrolysates with multifunctional (insulinotropic, dipeptidyl peptidase IV inhibitory and antioxidative) bioactive properties.

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Identification of dipeptidyl peptidase-IV inhibitory peptides from mare whey protein hydrolysates

Journal of Dairy Science ◽

10.3168/jds.2016-11828 ◽

2017 ◽

Vol 100 (9) ◽

pp. 6885-6894 ◽

Cited By ~ 18

Author(s):

J.J. Song ◽

Q. Wang ◽

M. Du ◽

X.M. Ji ◽

X.Y. Mao

Keyword(s):

Whey Protein ◽

Dipeptidyl Peptidase ◽

Protein Hydrolysates ◽

Dipeptidyl Peptidase Iv ◽

Inhibitory Peptides ◽

Whey Protein Hydrolysates

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Inhibitory properties of camel whey protein hydrolysates toward liver cancer cells, dipeptidyl peptidase-IV, and inflammation

Journal of Dairy Science ◽

10.3168/jds.2018-14586 ◽

2018 ◽

Vol 101 (10) ◽

pp. 8711-8720 ◽

Cited By ~ 14

Author(s):

Hina Kamal ◽

Sabika Jafar ◽

Priti Mudgil ◽

Chandraprabha Murali ◽

Amr Amin ◽

...

Keyword(s):

Liver Cancer ◽

Cancer Cells ◽

Whey Protein ◽

Dipeptidyl Peptidase ◽

Protein Hydrolysates ◽

Dipeptidyl Peptidase Iv ◽

Liver Cancer Cells ◽

Whey Protein Hydrolysates

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In Vitro Characterisation of the Antioxidative Properties of Whey Protein Hydrolysates Generated under pH- and Non pH-Controlled Conditions

Foods ◽

10.3390/foods9050582 ◽

2020 ◽

Vol 9 (5) ◽

pp. 582 ◽

Cited By ~ 3

Author(s):

Thanyaporn Kleekayai ◽

Aurélien V. Le Gouic ◽

Barbara Deracinois ◽

Benoit Cudennec ◽

Richard J. FitzGerald

Keyword(s):

Whey Protein ◽

Protein Hydrolysates ◽

Whey Protein Concentrate ◽

Trolox Equivalent Antioxidant Capacity ◽

Ros Generation ◽

Whey Protein Hydrolysates ◽

Hydrolysis Conditions ◽

The Impact ◽

Ph Controlled

Bovine whey protein concentrate (WPC) was hydrolysed under pH-stat (ST) and non pH-controlled (free-fall, FF) conditions using Debitrase (DBT) and FlavorPro Whey (FPW). The resultant whey protein hydrolysates (WPHs) were assessed for the impact of hydrolysis conditions on the physicochemical and the in vitro antioxidant and intracellular reactive oxygen species (ROS) generation in oxidatively stressed HepG2 cells. Enzyme and hydrolysis condition dependent differences in the physicochemical properties of the hydrolysates were observed, however, the extent of hydrolysis was similar under ST and FF conditions. Significantly higher (p < 0.05) in vitro and cellular antioxidant activities were observed for the DBT compared to the FPW–WPHs. The WPHs generated under ST conditions displayed significantly higher (p < 0.05) oxygen radical absorbance capacity (ORAC) and Trolox equivalent antioxidant capacity (TEAC) values compared to the FF-WPHs. The impact of hydrolysis conditions was more pronounced in the in vitro compared to the cellular antioxidant assay. WPH peptide profiles (LC-MS/MS) were also enzyme and hydrolysis conditions dependent as illustrated in the case of β-lactoglobulin. Therefore, variation in the profiles of the peptides released may explain the observed differences in the antioxidant activity. Targeted generation of antioxidant hydrolysates needs to consider the hydrolysis conditions and the antioxidant assessment method employed.

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