Enhanced action of apigenin and naringenin combination on estrogen receptor activation in non-malignant colonocytes: implications on sorghum-derived phytoestrogens

2015 ◽  
Vol 6 (3) ◽  
pp. 749-755 ◽  
Author(s):  
Liyi Yang ◽  
Kimberly F. Allred ◽  
Linda Dykes ◽  
Clinton D. Allred ◽  
Joseph M. Awika
Keyword(s):  
Estrogen Receptor ◽  
Young Adult ◽  
Adult Mouse ◽  
Receptor Activation

We report unusually strong enhanced effect of apigenin-naringenin combination and natural flavonoid mixtures on estrogenic response in non-malignant young adult mouse colonocytesin vitro.

In-vitro culture of periodontium of young adult mouse

1973 ◽  
Vol 18 (7) ◽  
pp. 933-939 ◽  
Author(s):  
A.H. Melcher ◽  
R.S. Turnbull ◽  
Wilma J. Hiddleston
Keyword(s):  
Young Adult ◽  
In Vitro Culture ◽  
Adult Mouse

scholarly journals Gut Microbiota and Phytoestrogen-Associated Infertility in Southern White Rhinoceros

mBio ◽  
2019 ◽  
Vol 10 (2) ◽  
Author(s):  
Candace L. Williams ◽  
Alexis R. Ybarra ◽  
Ashley N. Meredith ◽  
Barbara S. Durrant ◽  
Christopher W. Tubbs
Keyword(s):  
Mass Spectrometry ◽  
Estrogen Receptor ◽  
Gut Microbiota ◽  
Negative Relationship ◽  
Amplicon Sequencing ◽  
Receptor Activation ◽  
Activation Assay ◽  
White Rhinoceros ◽  
Southern White Rhinoceros

ABSTRACT With recent poaching of southern white rhinoceros (SWR [Ceratotherium simum simum]) reaching record levels, the need for a robust assurance population is urgent. However, the global captive SWR population is not currently self-sustaining due to the reproductive failure of captive-born females. Dietary phytoestrogens have been proposed to play a role in this phenomenon, and recent work has demonstrated a negative relationship between diet estrogenicity and fertility of captive-born female SWR. To further examine this relationship, we compared gut microbial communities, fecal phytoestrogens, and fertility of SWR to those of another rhinoceros species—the greater one-horned rhinoceros (GOHR [Rhinoceros unicornis]), which consumes a similar diet but exhibits high levels of fertility in captivity. Using 16S rRNA amplicon sequencing and mass spectrometry, we identified a species-specific fecal microbiota and three dominant fecal phytoestrogen profiles. These profiles exhibited various levels of estrogenicity when tested in an in vitro estrogen receptor activation assay for both rhinoceros species, with profiles dominated by the microbial metabolite equol stimulating the highest levels of receptor activation. Finally, we found that SWR fertility varies significantly not only with respect to phytoestrogen profile, but also with respect to the abundance of several bacterial taxa and microbially derived phytoestrogen metabolites. Taken together, these data suggest that in addition to species differences in estrogen receptor sensitivity to phytoestrogens, reproductive outcomes may be driven by the gut microbiota’s transformation of dietary phytoestrogens in captive SWR females. IMPORTANCE Southern white rhinoceros (SWR) poaching has reached record levels, and captive infertility has rendered SWR assurance populations no longer self-sustaining. Previous work has identified dietary phytoestrogens as a likely cause of this problem. Here, we investigate the role of gut microbiota in this phenomenon by comparing two rhinoceros species to provide the first characterizations of gut microbiomes for any rhinoceros species. To our knowledge, our approach, combining parallel sequencing, mass spectrometry, and estrogen receptor activation assays, provides insight into the relationship between microbially mediated phytoestrogen metabolism and fertility that is novel for any vertebrate species. With this information, we plan to direct future work aimed at developing strategies to improve captive reproduction in the hope of alleviating their threat of extinction.

scholarly journals Gut microbiota and phytoestrogen-associated infertility in southern white rhinoceros

2018 ◽  
Author(s):  
Candace L. Williams ◽  
Alexis R. Ybarra ◽  
Ashley N. Meredith ◽  
Barbara S. Durrant ◽  
Christopher W. Tubbs
Keyword(s):  
Estrogen Receptor ◽  
Negative Relationship ◽  
Amplicon Sequencing ◽  
Receptor Activation ◽  
Fecal Microbiota ◽  
16S Rrna Amplicon Sequencing ◽  
Activation Assay ◽  
White Rhinoceros ◽  
Southern White Rhinoceros

AbstractWith recent poaching of southern white rhinoceros (Ceratotherium simum simum; SWR) reaching record levels, the need for a robust assurance population is urgent. However, the global captive SWR population is not currently self-sustaining due to the reproductive failure of captive-born females. Dietary phytoestrogens have been proposed to play a role in this phenomenon, and recent work has demonstrated a negative relationship between diet estrogenicity and fertility of captive-born female SWR. To further examine this relationship, we compared gut microbial communities, fecal phytoestrogens, and fertility of SWR to another rhinoceros species–the greater one-horned rhinoceros (Rhinoceros unicornis; GOHR), which consumes a similar diet but exhibits high levels of fertility in captivity. Using 16S rRNA amplicon sequencing and mass spectrometry, we identified a species-specific fecal microbiota and three dominant fecal phytoestrogen profiles. These profiles exhibited varying levels of estrogenicity when tested in an in vitro estrogen receptor activation assay for both rhinoceros species, with profiles dominated by the microbial metabolite, equol, stimulating the highest levels of receptor activation. Finally, we found that SWR fertility varies significantly with respect to phytoestrogen profile, but also with the abundance of several bacterial taxa and microbially-derived phytoestrogen metabolites. Taken together, these data suggest that in addition to species differences in estrogen receptor sensitivity to phytoestrogens, reproductive outcomes may be driven by gut microbiota’s transformation of dietary phytoestrogens in captive SWR females.

scholarly journals Neuroprotection by Estrogens in a Mouse Model of Focal Cerebral Ischemia and in Cultured Neurons: Evidence for a Receptor-Independent Antioxidative Mechanism

1999 ◽  
Vol 19 (11) ◽  
pp. 1263-1269 ◽  
Author(s):  
Carsten Culmsee ◽  
Helmut Vedder ◽  
Alexander Ravati ◽  
Vera Junker ◽  
Dörte Otto ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Focal Cerebral Ischemia ◽  
Neuroprotective Effect ◽  
Radical Scavenging ◽  
Glucose Deprivation ◽  
Receptor Activation ◽  
17Β Estradiol ◽  
Brain Tissue Damage ◽  
Radical Scavenging Properties

Estrogens have been suggested for the treatment of neurodegenerative disorders, including stroke, because of their neuroprotective activities against various neurotoxic stimuli such as glutamate, glucose deprivation, iron, or β-amyloid. Here, the authors report that 17β-estradiol (0.3 to 30 mg/kg) and 2-OH-estradiol (0.003 to 30 mg/kg) reduced brain tissue damage after permanent occlusion of the middle cerebral artery in male NMRI mice. In vitro, 17β-estradiol (1 to 10 μmol/L) and 2-OH-estradiol (0.01 to 1 μmol/L) reduced the percentage of damaged chick embryonic neurons treated with FeSO4. In these primary neurons exposed to FeSO4, the authors also found reactive oxygen species to be diminished after treatment with 17β-estradiol (1 to 10 μmol/L) or 2-OH-estradiol (0.01 to 10 μmol/L), suggesting a strong antioxidant activity of the estrogens that were used. Neither the neuroprotective effect nor the free radical scavenging properties of the estrogens were influenced by the estrogen receptor antagonist tamoxifen. The authors conclude that estrogens protect neurons against damage by radical scavenging rather than through estrogen receptor activation.

scholarly journals In Vitro Chemopreventive Potential of Phlorotannins-Rich Extract from Brown Algae by Inhibition of Benzo[a]pyrene-Induced P2X7 Activation and Toxic Effects

Marine Drugs ◽  
2021 ◽  
Vol 19 (1) ◽  
pp. 34
Author(s):  
Mélody Dutot ◽  
Elodie Olivier ◽  
Sophie Fouyet ◽  
Romain Magny ◽  
Karim Hammad ◽  
...  
Keyword(s):  
Brown Algae ◽  
P2x7 Receptor ◽  
Biological Activities ◽  
Receptor Activation ◽  
Cytotoxic Effects ◽  
Human Lung Cells ◽  
Cytochrome P450 Activity ◽  
Species Production ◽  
E Cadherin

Phlorotannins are polyphenols occurring exclusively in some species of brown algae, known for numerous biological activities, e.g., antioxidant, antiproliferative, antidiabetic, and antiallergic properties. Their effects on the response of human lung cells to benzo[a]pyrene (B[a]P) has not been characterized. Our objective was to in vitro evaluate the effects of a phlorotannin-rich extract obtained from the brown algae Ascophyllum nodosum and Fucus vesiculosus on B[a]P cytotoxic effects. The A549 cell line was incubated with B[a]P for 48 and 72 h in the presence or absence of the brown algae extract. Cytochrome P450 activity, activation of P2X7 receptor, F-actin disorganization, and loss of E-cadherin expression were assessed using microplate cytometry and fluorescence microscopy. Relative to control, incubation with the brown algae extract was associated with lower B[a]P-induced CYP1 activity, lower P2X7 receptor activation, and lower reactive oxygen species production. The brown algae extract inhibited the alterations of F-actin arrangement and the downregulation of E-cadherin expression. We identified a phlorotannins-rich extract that could be deeper investigated as a cancer chemopreventive agent to block B[a]P-mediated carcinogenesis.

Mu-opioid receptor activation promotes in vitro and in vivo tumor growth in head and neck squamous cell carcinoma

Life Sciences ◽  
2021 ◽  
Vol 278 ◽  
pp. 119541
Author(s):  
Aysegul Gorur ◽  
Miguel Patiño ◽  
Hideaki Takahashi ◽  
German Corrales ◽  
Curtis R. Pickering ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Tumor Growth ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Opioid Receptor ◽  
Receptor Activation ◽  
Mu Opioid Receptor ◽  
Mu Opioid
Sign in / Sign up

Export Citation Format

Share Document