A comparison on the preparation of hot water extracts from Chlorella pyrenoidosa (CPEs) and radical scavenging and macrophage activation effects of CPEs

2014 ◽  
Vol 5 (12) ◽  
pp. 3252-3260 ◽  
Author(s):  
Xiuyuan Zhuang ◽  
Daojing Zhang ◽  
Wen Qin ◽  
Jia Deng ◽  
Hui Shan ◽  
...  
Keyword(s):  
High Pressure ◽  
Macrophage Activation ◽  
Growth Promotion ◽  
Radical Scavenging ◽  
Water Extract ◽  
Chlorella Pyrenoidosa ◽  
Free Radical Scavenging ◽  
Hot Water ◽  
Hot Water Extract

Hot water extract ofChlorella pyrenoidosa, prepared under high-pressure, was effective atin vitrofree radical scavenging and macrophage growth promotion.

scholarly journals Giloy (Tinospora Cordifolia): In Vitro Evaluation of Antibacterial Properties

2021 ◽  
Vol 07 (06) ◽  
Author(s):  
Tulsa Devi ◽  
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Water Extract ◽  
Hot Water ◽  
Tinospora Cordifolia ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Hot Water Extract ◽  
Agar Well Diffusion Method

Antibiotic resistance has become a global concern and hence, the search for other source of antimicrobials initiated to find a way to control infections in future. The main objective of this paper is to screen Giloy (Tinospora cordifolia) for its antibacterial activity. The stem of Tinospora cordifolia is used to prepare extract for determining it’s in vitro antibacterial activity as per the agar well diffusion method. In the agar well diffusion method 100μl of 24 hr broth culture of bacteria was aseptically and evenly swabbed on Mueller Hinton agar plates. Wells of about 8 mm diameter were aseptically cut using sterile cork-borer. 100 μl of plant extracts of different concentrations were then placed into the separate wells. The plates were incubated at 37 oC for 24hr. Antimicrobial activity of the giloy was determined by measuring the diameter of zone of inhibition. The methanolic extract of Tinospora cordifolia showed 13, 11, 9 and 5 mm zone of inhibition in S. aureus cultures by using 100, 75, 50 and 25 mg/ml concentration, respectively while hot water extract of Tinospora cordifolia showed 14, 12, 10 and 8 mm zone of inhibition for S. aureus by using 100, 75, 50 and 25 mg/ml concentration, respectively and the cold extract of Tinospora cordifolia showed 10, 8, 5 and 0 mm zone of inhibition for S. aureus by using 100, 75, 50 and 25mg/ml concentration, respectively. The methanolic extract of Tinospora cordifolia indicated 12, 10, 6 and 4 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25 mg/ml concentration, respectively and the hot water extract of Tinospora cordifolia showed 16, 14, 12 and 10 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25mg/ml concentration, respectively. The cold water extract of Tinospora cordifolia showed 13, 10, 8, and 5 mm zone of inhibition in cultures of E.coli by using 100, 75, 50 and 25 mg/ml concentration, respectively. It has been observed that Tinospora cordifolia showed very promising results as indicated by the zone of inhibition of bacterial culture through agar well diffusion method that varies from few mm to few cm. This study indicates the in-vitro antibacterial effect of Giloy which needs further validation through in-vivo studies.

scholarly journals Radical-Scavenging Activity of Hot Water Extract from Leaves of Sweet Potato Cultivar "Simon-1".

2002 ◽  
Vol 49 (10) ◽  
pp. 683-687 ◽  
Author(s):  
Tomoyuki Oki ◽  
Mami Masuda ◽  
Miyuki Osame ◽  
Mio Kobayashi ◽  
Shu Furuta ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Extract ◽  
Potato Cultivar ◽  
Hot Water ◽  
Scavenging Activity ◽  
Hot Water Extract

scholarly journals Anti-inflammatory Effect of Onion (Allium cepa) Peel Hot Water Extract in vitro and in vivo

KSBB Journal ◽  
2015 ◽  
Vol 30 (4) ◽  
pp. 148-154
Author(s):  
Bo-Kyeong Kang ◽  
Koth-Bong-Woo-Ri Kim ◽  
Na-Kyung Ahn ◽  
Yeon-Uk Choi ◽  
Min-ji Kim ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Water Extract ◽  
Hot Water ◽  
Hot Water Extract ◽  
Anti Inflammatory ◽  
Inflammatory Effect

scholarly journals Assessment of Cosmeceutical Potentials of Selected Mushroom Fruitbody Extracts Through Evaluation of Antioxidant, Anti-Hyaluronidase and Anti-Tyrosinase Activity

J ◽  
2020 ◽  
Vol 3 (3) ◽  
pp. 329-342
Author(s):  
Dang Lelamurni Abd Razak ◽  
Anisah Jamaluddin ◽  
Nur Yuhasliza Abd Rashid ◽  
Nor Ajila Sani ◽  
Musaalbakri Abdul Manan
Keyword(s):  
Cold Water ◽  
Schizophyllum Commune ◽  
Radical Scavenging ◽  
Water Extract ◽  
Antioxidant Property ◽  
Tyrosinase Activity ◽  
Hot Water ◽  
Scavenging Activity ◽  
Hyaluronidase Activity ◽  
Hot Water Extract

Cosmeceutical formulations containing naturally derived active ingredients are currently preferred by consumers worldwide. Mushrooms are one of the potential sources for cosmeceutical ingredients but relevant research is still lacking. In this study, hot- and cold-water extractions were performed on four locally-cultivated mushrooms—Pleurotus ostreatus, Ganoderma lucidum, Auricularia polytricha and Schizophyllum commune—with the aim to assess the cosmeceutical potential of these mushroom fruitbody extracts. Total phenolics, polysaccharide and glucan content were determined. Antioxidant property of the mushroom extracts was assessed by determining the DPPH radical scavenging, ferric-reducing (FRAP) and superoxide anion (SOA) scavenging activity. Anti-hyaluronidase activity was used as an indicator for the anti-aging and anti-inflammatory property, while anti-tyrosinase activity was evaluated to assess the anti-pigmentation or whitening property of these extracts. Our results showed that total polysaccharide content of P. ostreatus extracts was the highest (235.8–253.6 mg GE/g extract), while extracts from G. lucidum contained the lowest glucan (10.12–10.67%). Cold-water extract from S. commune exhibited substantial tyrosinase inhibition activity (98.15%) and SOA scavenging activity (94.82%). The greatest hyaluronidase activity was exhibited by G. lucidum hot-water extract, with the value of 72.78%. The findings from the correlation analyses suggest that the cosmeceutical properties of these mushrooms can be attributed mainly to the combination of different types of compound such as polysaccharides and phenolics. Overall, cold-water extract of S. commune and hot-water extract of G. lucidum showed the best results and may be further investigated.

scholarly journals Skin Antiaging Effects of the Fermented Outer Layers of Leaf Skin of Aloe barbadensis Miller Associated with the Enhancement of Mitochondrial Activities of UVb-Irradiated Human Skin Fibroblasts

2021 ◽  
Vol 11 (12) ◽  
pp. 5660
Author(s):  
Hyeonwoo Lee ◽  
Woonyong Choi ◽  
Hyangseon Ro ◽  
Gyurae Kim ◽  
Hyeonyong Lee
Keyword(s):  
Human Skin ◽  
Radical Scavenging ◽  
Aloe Vera ◽  
Water Extract ◽  
Hot Water ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Bioactive Substances ◽  
Aloe Barbadensis ◽  
Hot Water Extract

This study is the first to show that increased mitochondrial activities improved the antiaging effects of Aloe vera leaf skin fermented by Lactobacillus plantarum on UVb-irradiated skin fibroblasts. The fermented extract (AF) increased the activities of mitochondrial reductase and the complex II and significantly reduced reactive oxygen species (ROS) production, even under UVb stress conditions, and also increased DPPH free radical scavenging activities compared with the hot water extract of outer layers of aloe leaf (AW) and quercetin itself. AF exerted a synergistic effect with quercetin and bioactive substances derived from the fermentation process. Moreover, mitochondrial activation of UVb-irradiated human skin fibroblasts by 0.3% (w/v) of the AF plays important roles in increasing collagen production up to 125 ± 5.45% and decreasing MMP-1 secretion down to 69.41 ± 2.63% of the control levels. The AF enhanced the upregulation of collagen gene expression, and this change was also greater than those induced by the AW and quercetin. Therefore, this study concludes that fermentation of the skin of aloe leaves increases the activation of mitochondria and inhibits the photo-aging of UVb-irradiated skin fibroblasts.

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