In vitro digestion of citric acid esters of mono- and diglycerides (CITREM) and CITREM-containing infant formula/emulsions

2014 ◽  
Vol 5 (7) ◽  
pp. 1409-1421 ◽  
Author(s):  
Sawsan Amara ◽  
Amaury Patin ◽  
Francesca Giuffrida ◽  
Tim J. Wooster ◽  
Sagar K. Thakkar ◽  
...  
Keyword(s):  
Citric Acid ◽  
Infant Formula ◽  
In Vitro Digestion ◽  
First Time ◽  
Digestive Lipases ◽  
Acid Esters

The GI lipolysis of CITREM is investigated for the first time using various digestive lipases and a two-stepin vitrodigestion model.

α-Glucosidase Inhibition and Docking Studies of 5-Deoxyflavonols and Dihydroflavonols Isolated from Abutilon pakistanicum

2019 ◽  
Vol 23 (17) ◽  
pp. 1857-1866
Author(s):  
Munawar Hussain ◽  
Zaheer Ahmed ◽  
Shamsun N. Khan ◽  
Syed A. A. Shah ◽  
Rizwana Razi ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Docking Studies ◽  
Hydroxyl Group ◽  
Coumaric Acid ◽  
In Vitro Activity ◽  
Aerial Parts ◽  
First Time ◽  
Acid Esters ◽  
Phenol Moiety

Three new 5-deoxyflavonoid and dihydroflavonoids 2, 3 and 4 have been isolated from the methanolic extract of Abutioln pakistanicum aerial parts, for which structures were elucidated explicitly by extensive MS- and NMR-experiments. In addition to these, 3,7,4′-trihydroxy-3′-methoxy flavonol (1) is reported for the first time from Abutioln pakistanicum. Compound 2 and 4 are p-coumaric acid esters while compounds 2–4 exhibited α-glucosidase inhibitory activity. Docking studies indicated that the ability of flavonoids 2, 3 and 4 to form multiple hydrogen bonds with catalytically important residues is decisive hence is responsible for the inhibition activity. The docking results signified the observed in-vitro activity quite well which is in accordance with previously obtained conclusion that phenol moiety and hydroxyl group are critical for the inhibition of α-glucosidase enzyme.

In vitro digestion of infant formula model systems: Influence of casein to whey protein ratio

2021 ◽  
Vol 117 ◽  
pp. 105008
Author(s):  
Arissara Phosanam ◽  
Jayani Chandrapala ◽  
Thom Huppertz ◽  
Benu Adhikari ◽  
Bogdan Zisu
Keyword(s):  
Whey Protein ◽  
Infant Formula ◽  
In Vitro Digestion ◽  
Model Systems ◽  
Protein Ratio

Effect of Pepsin Treatment on the Chemical Iron Profile of Soy-Based Foods Supplemented with Selected Iron Sources and Enhancers

1985 ◽  
Vol 48 (1) ◽  
pp. 35-38 ◽  
Author(s):  
S.W. RIZK ◽  
F.M. CLYDESDALE
Keyword(s):  
Ascorbic Acid ◽  
Citric Acid ◽  
Degradation Products ◽  
In Vitro Digestion ◽  
Soy Milk ◽  
Pepsin Digestion ◽  
Iron Solubility ◽  
Iron Profile ◽  
Solubilizing Capacity

Changes in chemical iron profile occurring from pH 2 to 6.5 in a wheat-soy blend, a corn-soy-milk mix, and a soy-extended beef patty were investigated. Iron solubility in these products, as affected by in vitro digestion with pepsin, was dependent on a combination of ligand, iron source, pH and food. The greatest solubilizing capacity of the ligands added was provided by ascorbic acid at pH 2 and 4, and by citric acid at pH 6. Improvements in percent soluble iron were related to pepsin digestion and the presumed appearance of protein degradation products.

scholarly journals Impact of Acetic Acid on the Survival ofL. plantarumupon Microencapsulation by Coaxial Electrospraying

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Laura G. Gómez-Mascaraque ◽  
Jesús Ambrosio-Martín ◽  
Rocío Perez-Masiá ◽  
Amparo Lopez-Rubio
Keyword(s):  
Acetic Acid ◽  
Inner Core ◽  
In Vitro Digestion ◽  
Probiotic Bacteria ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Outer Solution ◽  
Protein Particles ◽  
First Time

In this work, coaxial electrospraying was used for the first time to microencapsulate probiotic bacteria, specificallyLactobacillus plantarum, within edible protein particles with the aim of improving their resistance to in vitro digestion. The developed structures, based on an inner core of whey protein concentrate and an outer layer of gelatin, were obtained in the presence of acetic acid in the outer solution as a requirement for the electrospraying of gelatin. Despite the limited contact of the inner suspension and outer solution during electrospraying, the combination of the high voltage used during electrospraying with the presence of acetic acid was found to have a severe impact on the lactobacilli, not only decreasing initial viability but also negatively affecting the survival of the bacteria during storage and their resistance to different stress conditions, including simulated in vitro digestion.

scholarly journals Decreased Citrate Improves Iron Availability from Infant Formula: Application of an In Vitro Digestion/Caco-2 Cell Culture Model

1998 ◽  
Vol 128 (2) ◽  
pp. 257-264 ◽  
Author(s):  
Raymond P. Glahn ◽  
Cindy Lai ◽  
Jean Hsu ◽  
John F. Thompson ◽  
Mingruo Guo ◽  
...  
Keyword(s):  
Cell Culture ◽  
Infant Formula ◽  
In Vitro Digestion ◽  
Iron Availability ◽  
Cell Culture Model ◽  
Culture Model

Development of antioxidant gliadin particle stabilized Pickering high internal phase emulsions (HIPEs) as oral delivery systems and the in vitro digestion fate

2018 ◽  
Vol 9 (2) ◽  
pp. 959-970 ◽  
Author(s):  
F. Z. Zhou ◽  
T. Zeng ◽  
S. W. Yin ◽  
C. H. Tang ◽  
D. B. Yuan ◽  
...  
Keyword(s):  
Lipid Oxidation ◽  
Chemical Stability ◽  
Oral Delivery ◽  
In Vitro Digestion ◽  
Delivery Systems ◽  
Algal Oil ◽  
Internal Phase ◽  
First Time ◽  
Oral Delivery Systems

In this paper, we demonstrate for the first time the use of gliadin particles to structure algal oil (rich in DHA) and to exert chemical stability against lipid oxidation via the Pickering high internal phase emulsion (HIPE) strategy.

scholarly journals Moringa oleifera—Storage Stability, In Vitro-Simulated Digestion and Cytotoxicity Assessment of Microencapsulated Extract

Processes ◽  
2020 ◽  
Vol 8 (7) ◽  
pp. 770
Author(s):  
Cecilia Castro-López ◽  
Catarina Gonçalves ◽  
Janeth M. Ventura-Sobrevilla ◽  
Lorenzo M. Pastrana ◽  
Cristóbal N. Aguilar-González ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
In Vitro Digestion ◽  
Storage Conditions ◽  
Simulated Digestion ◽  
A Cell ◽  
Total Polyphenolic Content ◽  
Cytotoxicity Assessment ◽  
First Time ◽  
And Storage

Moringa extract was microencapsulated for the first time by spray-drying technique using tragacanth gum (MorTG) to improve its stability under gastrointestinal and storage conditions, assessing total polyphenolic content (TPC) and antioxidant activity. Additionally, cytotoxicity of the microencapsulated components was evaluated after contact with Caco-2 cells. Results showed that TPC was released as follows—oral (9.7%) < gastric (35.2%) < intestinal (57.6%). In addition, the antioxidant activity in in vitro digestion reached up to 16.76 ±0.15 mg GAE g−1, which was 300% higher than the initial value. Furthermore, microencapsulated moringa extract presented a half-life up to 45 days of storage, where the noticeably change was observed at 35 °C and 52.9% relative humidity. Finally, direct treatment with 0.125 mg mL−1 MorTG on Caco-2 cells showed a slight antiproliferative effect, with a cell viability of approx. 87%. Caco-2 cells’ viability demonstrated non-cytotoxicity, supporting the safety of the proposed formulation and potential use within the food field.

scholarly journals A Way to Increase the Bioaccesibility and Photostability of Roflumilast, a COPD Treatment, by Cyclodextrin Monomers

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 801 ◽  
Author(s):  
Adrián Matencio ◽  
Samanta Hernández-García ◽  
Francisco García-Carmona ◽  
José Manuel López-Nicolás
Keyword(s):  
Adverse Effects ◽  
In Vitro Digestion ◽  
Chronic Obstructive ◽  
Fluorescence Signal ◽  
Complexation Constant ◽  
Chronic Obstructive Pulmonary Diseases ◽  
Increasing Temperature ◽  
First Time ◽  
Low Solubility

Roflumilast is an orally available inhibitor of phosphodiesterase (PDE) type 4, which is widely used in chronic obstructive pulmonary diseases. However, it has low solubility and adverse effects include diarrhea and nausea. Since its solubilization may improve treatment and, dismissing any adverse effects, its interaction with cyclodextrins (CDs) was studied. The Higuchi-Connors method was used to determine the complexation constant with different CDs, pH values and temperatures. Molecular docking was used to predict interaction between the complexes. An in vitro digestion experiment was carried out to test roflumilast protection. Finally, the photostability of the complex was evaluated. The complex formed with β-CD had the highest K11 value (646 ± 34 M−1), although this value decreased with increasing temperature. Similarly, K11 decreased as the pH increased. In vitro digestion showed that CDs protect the drug during digestion and even improve its bioaccessibility. Finally, CDs reduced the drug’s extreme photosensitivity, originating a fluorescence signal, which is described for first time. The kinetic parameters of the reaction were obtained. This study not only completes the complexation study of roflumilast-CD, but also points to the need to protect roflumilast from light, suggesting that tablets containing the drug might be reformulated.

scholarly journals A comprehensive review on in vitro digestion of infant formula

2015 ◽  
Vol 76 ◽  
pp. 373-386 ◽  
Author(s):  
Thao T.P. Nguyen ◽  
Bhesh Bhandari ◽  
Julie Cichero ◽  
Sangeeta Prakash
Keyword(s):  
Infant Formula ◽  
In Vitro Digestion ◽  
Comprehensive Review
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