Sensitivity enhancement by population transfer in Gd(iii) spin labels

2015 ◽  
Vol 17 (11) ◽  
pp. 7334-7344 ◽  
Author(s):  
Andrin Doll ◽  
Mian Qi ◽  
Stephan Pribitzer ◽  
Nino Wili ◽  
Maxim Yulikov ◽  
...  
Keyword(s):  
Electron Spin ◽  
Sensitivity Enhancement ◽  
Spin Labels ◽  
Population Transfer ◽  
Distance Measurements ◽  
Spin Populations

Frequency-swept chirp pulses with bandwidths > 1 GHz rearrange electron spin populations in the S = 7/2 spin of Gd(iii) ions for better sensitivity, as demonstrated with distance measurements between Gd(iii) ions.

scholarly journals Room Temperature Electron Spin Resonance Distance Measurements in T4 Lysozyme using Trityl-Based Spin Labels

2012 ◽  
Vol 102 (3) ◽  
pp. 405a ◽  
Author(s):  
Zhongyu Yang ◽  
Yangping Liu ◽  
Peter Borbat ◽  
Jay Zweier ◽  
Jack Freed ◽  
...  
Keyword(s):  
Electron Spin Resonance ◽  
Electron Spin ◽  
Room Temperature ◽  
Spin Labels ◽  
T4 Lysozyme ◽  
Distance Measurements ◽  
Spin Resonance ◽  
Temperature Electron

Unfolding of Alanine-Based Peptides Using Electron Spin Resonance Distance Measurements†

Biochemistry ◽  
2006 ◽  
Vol 45 (38) ◽  
pp. 11666-11673 ◽  
Author(s):  
Sangmi Jun ◽  
James S. Becker ◽  
Mike Yonkunas ◽  
Rob Coalson ◽  
Sunil Saxena
Keyword(s):  
Electron Spin Resonance ◽  
Electron Spin ◽  
Distance Measurements ◽  
Spin Resonance

Versatile Trityl Spin Labels for Nanometer Distance Measurements on Biomolecules In Vitro and within Cells

2016 ◽  
Vol 56 (1) ◽  
pp. 177-181 ◽  
Author(s):  
J. Jacques Jassoy ◽  
Andreas Berndhäuser ◽  
Fraser Duthie ◽  
Sebastian P. Kühn ◽  
Gregor Hagelueken ◽  
...  
Keyword(s):  
Spin Labels ◽  
Distance Measurements

Triplet Fullerenes as Prospective Spin Labels for Nanoscale Distance Measurements by Pulsed Dipolar EPR Spectroscopy

2019 ◽  
Vol 131 (38) ◽  
pp. 13405-13409 ◽  
Author(s):  
Olesya A. Krumkacheva ◽  
Ivan O. Timofeev ◽  
Larisa V. Politanskaya ◽  
Yuliya F. Polienko ◽  
Evgeny V. Tretyakov ◽  
...  
Keyword(s):  
Epr Spectroscopy ◽  
Spin Labels ◽  
Distance Measurements

scholarly journals Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Eliane H. Yardeni ◽  
Thorsten Bahrenberg ◽  
Richard A. Stein ◽  
Smriti Mishra ◽  
Elia Zomot ◽  
...  
Keyword(s):  
Solution Structure ◽  
Spin Labels ◽  
Multidrug Transporter ◽  
Distance Measurements ◽  
E Coli

A new approach to distance measurements between two spin labels in the >10 nm range

2017 ◽  
Vol 19 (7) ◽  
pp. 5222-5229 ◽  
Author(s):  
A. Blank
Keyword(s):  
Esr Spectroscopy ◽  
Dipolar Interaction ◽  
Spin Labels ◽  
Interaction Frequency ◽  
Distance Measurements ◽  
New Approach ◽  
Nm Range

ESR spectroscopy can be efficiently used to acquire the distance between two spin labels placed on a macromolecule by measuring their mutual dipolar interaction frequency, as long as the distance is not greater than ∼10 nm.

Pulsed electron–electron double resonance: beyond nanometre distance measurements on biomacromolecules

2011 ◽  
Vol 434 (3) ◽  
pp. 353-363 ◽  
Author(s):  
Gunnar W. Reginsson ◽  
Olav Schiemann
Keyword(s):  
Protein Complexes ◽  
Double Resonance ◽  
Resonance Method ◽  
Spin Labels ◽  
Coupling Mechanism ◽  
Distance Measurements ◽  
Paramagnetic Resonance ◽  
Distance Distributions ◽  
Nitroxide Spin Labels ◽  
Amino Acid Radicals

PELDOR (or DEER; pulsed electron–electron double resonance) is an EPR (electron paramagnetic resonance) method that measures via the dipolar electron–electron coupling distances in the nanometre range, currently 1.5–8 nm, with high precision and reliability. Depending on the quality of the data, the error can be as small as 0.1 nm. Beyond mere mean distances, PELDOR yields distance distributions, which provide access to conformational distributions and dynamics. It can also be used to count the number of monomers in a complex and allows determination of the orientations of spin centres with respect to each other. If, in addition to the dipolar through-space coupling, a through-bond exchange coupling mechanism contributes to the overall coupling both mechanisms can be separated and quantified. Over the last 10 years PELDOR has emerged as a powerful new biophysical method without size restriction to the biomolecule to be studied, and has been applied to a large variety of nucleic acids as well as proteins and protein complexes in solution or within membranes. Small nitroxide spin labels, paramagnetic metal ions, amino acid radicals or intrinsic clusters and cofactor radicals have been used as spin centres.

Generic tags for Mn(ii) and Gd(iii) spin labels for distance measurements in proteins

2017 ◽  
Vol 19 (39) ◽  
pp. 26944-26956 ◽  
Author(s):  
Yin Yang ◽  
Yan-Jun Gong ◽  
Aleksei Litvinov ◽  
Hong-Kai Liu ◽  
Feng Yang ◽  
...  
Keyword(s):  
Spin Label ◽  
Metal Ion ◽  
Coordination Mode ◽  
Distance Distribution ◽  
Spin Labels ◽  
Distance Measurements

The coordination mode of the metal ion in the spin label affects the distance distribution determined by DEER distance measurements.

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