scholarly journals DNA mediated assembly of quantum dot–protoporphyrin IX FRET probes and the effect of FRET efficiency on ROS generation

2015 ◽  
Vol 17 (8) ◽  
pp. 5973-5981 ◽  
Author(s):  
Seema Singh ◽  
Anirban Chakraborty ◽  
Vandana Singh ◽  
Aniruddha Molla ◽  
Sahid Hussain ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Dna Hybridization ◽  
Protoporphyrin Ix ◽  
Ros Generation ◽  
Fret Efficiency

Quantum dot-protoporphyrin IX FRET probes are assembled through DNA hybridization and the efficiency of FRET and ROS generation was studied.

A quantum dot–MUC1 aptamer conjugate for targeted delivery of protoporphyrin IX and specific photokilling of cancer cells through ROS generation

2016 ◽  
Vol 8 (10) ◽  
pp. 1040-1048 ◽  
Author(s):  
Seema Singh ◽  
Pravin Jha ◽  
Vandana Singh ◽  
Kislay Sinha ◽  
Sahid Hussain ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Cancer Cells ◽  
Targeted Delivery ◽  
Protoporphyrin Ix ◽  
Ros Generation ◽  
Muc1 Aptamer

A prototype DNA assembled quantum dot-photosensitizer (PS) nanodevice for targeted delivery of PS drug.

Flexible Microfabricated Film Sensors for the in Situ Quantum Dot-Based Voltammetric Detection of DNA Hybridization in Microwells

2014 ◽  
Vol 87 (2) ◽  
pp. 853-857 ◽  
Author(s):  
Christos Kokkinos ◽  
Anastasios Economou ◽  
Thanasis Speliotis ◽  
Panagiota Petrou ◽  
Sotirios Kakabakos
Keyword(s):  
Quantum Dot ◽  
Dna Hybridization ◽  
Voltammetric Detection

scholarly journals Photophysics of Titania Nanoparticle/Quantum Dot Hybrid Structures-=SUP=-*-=/SUP=-

2020 ◽  
Vol 128 (8) ◽  
pp. 1197
Author(s):  
E.P. Kolesova ◽  
F.M. Safin ◽  
V.G. Maslov ◽  
A. Dubavik ◽  
Y.K. Gun'ko ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Electron Transfer ◽  
Quantum Dot ◽  
Electron Donor ◽  
Small Diameter ◽  
Transfer Efficiency ◽  
Titania Nanoparticles ◽  
Hybrid Structures ◽  
Ros Generation ◽  
Cdse Qds

The efficiency of the electron transfer in hybrid structures based on quantum dots of different architectures was studied. Electron transfer efficiency was estimated by two independent methods from the side of the electron donor (quantum dot) and acceptor (Titania nanoparticles). Structures based on core CdSe QDs with small diameter demonstrate the highest efficiency of electron transfer and ROS generation. The presence of the dark fraction of QDs in the ensemble reduces the functionality of hybrid structures and limits their practical applicability.

Quantum-Dot/Dendrimer Based Functional Nanotubes for Sensitive Detection of DNA Hybridization

2008 ◽  
pp. 84-90
Author(s):  
Chuan Liang Feng ◽  
Ya Ming Yu ◽  
Xin Hua Zhong ◽  
Martin Steinhart ◽  
Jean P. Majoral ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Dna Hybridization ◽  
Sensitive Detection

scholarly journals The importance of iron chelation and iron availability during PpIX-induced photodynamic therapy

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Alison Curnow ◽  
Andrew Pye
Keyword(s):  
Dna Damage ◽  
Photodynamic Therapy ◽  
Protoporphyrin Ix ◽  
Iron Chelation ◽  
Good Effect ◽  
Ros Generation ◽  
Iron Availability ◽  
Concurrent Administration ◽  
Trypan Blue Exclusion ◽  
Iron Sulphate

AbstractBackground:Protoporphyrin IX (PpIX)-induced photodynamic therapy (PDT) is being utilised as a topical method of localised ablation of certain non-melanoma skin cancers and precancers. Standardised protocols have been implemented to good effect when the disease remains superficial but improvement is required to treat thicker or acrally located conditions. Concurrent administration of an iron chelator during PpIX-PDT has been demonstrated to increase cellular accumulation of PpIX by reducing its bioconversion to haem (an iron dependent process) thus increasing cell kill on subsequent irradiation. Iron however, can also play a role in reactive oxygen species (ROS) generation and limiting its availability via chemical chelation could theoretically reduce the efficacy of PpIX-PDT, so that a response less than that maximally feasible is produced.Materials and methods:The effects of iron availability and chelation on PpIX-PDT have therefore been investigated via fluorescence quantification of PpIX accumulation, single-cell gel electrophoresis (comet assay) measurement of ROS-induced DNA damage and trypan blue exclusion assessment of cell viability. Cultured human cells were utilised and incubated in standardised iron conditions with the PpIX precursor’s aminolaevulinic acid (ALA) or its methyl ester (MAL) in the presence or absence of either of the iron chelating agents desferrioxamine (DFO) or hydroxypyridinone (CP94), or alternatively iron sulphate as a source of iron.Results:ALA or MAL incubation was found to significantly increase cellular PpIX accumulation pre-irradiation as anticipated and this observation correlated with both significantly increased DNA damage and reduced cellular viability following irradiation. Co-incubation with either of the iron chelators investigated (DFO or CP94) significantly increased pre-irradiation PpIX accumulation as well as DNA damage and cell death on irradiation indicating the positive effect of iron chelation on the effectiveness of PpIX-induced PDT. The opposite effects were observed however, when the cells were co-incubated with iron sulphate, with significant reductions in pre-irradiation PpIX accumulation (ALA only) and DNA damage (ALA and MAL) being recorded indicating the negative effects excessive iron can have on PpIX-PDT effectiveness. Some dark toxicity produced by iron sulphate administration in non-irradiated control groups was also observed.Conclusion:Iron chelation and availability have therefore been observed to positively and adversely affect the PpIX-PDT process respectively and it is concluded that the effects of increased PpIX accumulation pre-irradiation produced via iron chelation outweigh any limitations reduced iron availability may have on the ability of iron to catalyse ROS generation/cascades following PpIX-induced PDT. Further investigation of iron chelation within dermatological applications where enhanced PpIX-PDT treatment effects would be beneficial is therefore warranted.

Quantum dot-based DNA hybridization by electrochemiluminescence and anodic stripping voltammetry

The Analyst ◽  
2010 ◽  
Vol 135 (7) ◽  
pp. 1773 ◽  
Author(s):  
Haiping Huang ◽  
Jingjing Li ◽  
Yanglan Tan ◽  
Jinjun Zhou ◽  
Jun-Jie Zhu
Keyword(s):  
Quantum Dot ◽  
Dna Hybridization ◽  
Anodic Stripping Voltammetry ◽  
Stripping Voltammetry ◽  
Anodic Stripping
Sign in / Sign up

Export Citation Format

Share Document