A universal sensing platform based on the repair ligation-mediated light-producing DNA machine

2015 ◽  
Vol 51 (26) ◽  
pp. 5652-5655 ◽  
Author(s):  
Qinfeng Xu ◽  
Yan Zhang ◽  
Chun-yang Zhang
Keyword(s):  
Sensing Platform ◽  
Dna Machine ◽  
Dna Machines

We developed a universal sensing platform based on the repair ligation-mediated light-producing DNA machines.

scholarly journals A proteinase-free DNA replication machinery for in vitro and in vivo amplified MicroRNA imaging

2020 ◽  
Vol 48 (10) ◽  
pp. e60-e60 ◽  
Author(s):  
Jie Wei ◽  
Huimin Wang ◽  
Xue Gong ◽  
Qing Wang ◽  
Hong Wang ◽  
...  
Keyword(s):  
Dna Replication ◽  
Living Cells ◽  
Hybridization Chain Reaction ◽  
Replication Machinery ◽  
Chain Reaction ◽  
Free Dna ◽  
Sensing Platform ◽  
Dna Machine

Abstract The construction of robust, modular and compact DNA machinery facilitates us to build more intelligent and ingenious sensing strategies in complex biological systems. However, the performance of conventional DNA amplifiers is always impeded by their limited in-depth amplifications and miscellaneously enzymatic requirements. Here, a proteinase-free reciprocal DNA replication machinery is developed by exploiting the synergistic cross-activation between hybridization chain reaction (HCR) and DNAzyme. The DNAzyme provides an efficient way to simplify the sophisticated design of HCR machinery and simultaneously to promote the amplification capacity. And the HCR-assembled tandem DNAzyme nanowires produce numerous new triggers for reversely stimulating HCR amplifier as systematically explored by experiments and computer-aided simulations. The reciprocal amplifier can be executed as a versatile and powerful sensing platform for analyzing miRNA in living cells and even in mice, originating from the inherent reaction accelerations and multiple-guaranteed recognitions. The reciprocal catalytic DNA machine holds great potential in clinical diagnosis and assessment.

Optical Sensors for Detection of Amino Acids

2018 ◽  
Vol 25 (19) ◽  
pp. 2272-2290 ◽  
Author(s):  
Aafrin M. Pettiwala ◽  
Prabhat K. Singh
Keyword(s):  
Amino Acids ◽  
Optical Sensors ◽  
Supramolecular Assemblies ◽  
Significant Activity ◽  
Physiological Level ◽  
Biological Scaffolds ◽  
Detection Systems ◽  
Sensing Platform ◽  
Neurological Conditions ◽  
Detailed Search

Background: Amino acids are crucially involved in a myriad of biological processes. Any aberrant changes in physiological level of amino acids often manifest in common metabolic disorders, serious neurological conditions and cardiovascular diseases. Thus, devising methods for detection of trace amounts of amino acids becomes highly elemental to their efficient clinical diagnosis. Recently, the domain of developing optical sensors for detection of amino acids has witnessed significant activity which is the focus of the current review article. Methods: We undertook a detailed search of the peer-reviewed literature that primarily deals with optical sensors for amino acids and focuses on the use of different type of materials as a sensing platform. Results: Ninety-five papers have been included in the review, majority of which deal with optical sensors. We attempt to systematically classify these contributions based on the applications of various chemical and biological scaffolds such as polymers, supramolecular assemblies, nanoparticles, DNA, heparin etc for the sensing of amino acids. This review identifies that supramolecular assemblies and nanomaterial continue to be commonly used platforms to devise sensors for amino acids followed by surfactant assemblies. Conclusion: The broad implications of amino acids in human health and diagnosis have stirred a lot of interest to develop optimized optical detection systems for amino acids in recent years, using different materials based on chemical and biological scaffolds. We have also attempted to highlight the merits and demerits of some of the noteworthy sensor systems to instigate further efforts for constructing amino acids sensor based on unconventional concepts.

Selective Sensing Platform Utilizing Graphitized Multi-Walled Carbon Nanotubes for Monitoring of Ondansetron and Paracetamol

2020 ◽  
Vol 16 ◽  
Author(s):  
Biljana Nigović ◽  
Iva Šimunić ◽  
Ana Mornar
Keyword(s):  
Carbon Nanotubes ◽  
Cation Exchange ◽  
Supporting Electrolyte ◽  
Polymer Concentration ◽  
Fixed Dose ◽  
Sensing Platform ◽  
Multi Walled Carbon Nanotubes ◽  
Electroanalytical Method ◽  
Walled Carbon Nanotubes

Background: Ondansetron and paracetamol are often co-administrated to prevent and treat nausea and vomiting caused by anaesthesia and to control of postoperative pain. In addition, ondansetron is used as the first-line antiemetic in paracetamol overdose. Therefore, selective and sensitive method for their simultaneous analysis is of a great importance. The electroanalytical methods are highly sensitive and offer many possibilities for new sensor platform design. However, at present, no electroanalytical method for simultaneous determination of these drugs has been proposed. Objective: The aim of this study was to develop a novel nanosensor for selective monitoring of ondansetron and paracetamol in pharmaceutical and biological samples without expensive and time-consuming pretreatments. Methods: The graphitized multi-walled carbon nanotubes embedded in a cation exchange polymer matrix was selected, among various surface functionalizations evaluated, to design novel sensor. Based on its excellent sensing performance, the first electroanalytical method was developed for rapid concurrent determination of investigated drugs. Results: The scanning electron microscopy study showed interlinked nanoporous network structure and highly enlarged active surface. The developed sensor facilitated electron transfer in the oxidation of both drugs and tremendously enhanced the adsorption capacity for ondasetron, thus exhibiting significant increase of drug responses and sensitivity. To obtain much sensitive response of investigated drugs the effect of pH values of supporting electrolyte, dispersed nanomaterial amount, the cation exchange polymer concentration, drop-casting volume of nanocomposite suspension, accumulation potential and deposition time on the peak current was evaluated. The developed electroanalytical method was validated and practical utility of the proposed nanosensor was tested. Conclusion: The developed sensor is promising sensing platform with a fast response time for analysis of ondansetron and paracetamol at very different concentration levels found in their fixed-dose combination and human serum sample after recommended daily doses showing its potential usage in pharmaceutical quality control and clinical research.

Colorimetric Dye-Loaded Nanofiber Yarn: Eye-Readable and Weavable Gas Sensing Platform

ACS Nano ◽  
2020 ◽  
Vol 14 (12) ◽  
pp. 16907-16918
Author(s):  
Dong-Ha Kim ◽  
Jun-Hwe Cha ◽  
Jee Young Lim ◽  
Jaehyeong Bae ◽  
Woosung Lee ◽  
...  
Keyword(s):  
Gas Sensing ◽  
Nanofiber Yarn ◽  
Sensing Platform
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