Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection

2015 ◽  
Vol 51 (14) ◽  
pp. 2954-2956 ◽  
Author(s):  
Zhen-Kun Wu ◽  
Dian-Ming Zhou ◽  
Zhan Wu ◽  
Xia Chu ◽  
Ru-Qin Yu ◽  
...  
Keyword(s):  
High Specificity ◽  
Single Base ◽  
Single Base Mismatch ◽  
Base Mismatch ◽  
Mismatch Discrimination ◽  
First Time ◽  
T7 Exonuclease

T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch.

Detection of p53 Gene Mutation (Single-Base Mismatch) Using a Fluorescent Silver Nanoclusters

2017 ◽  
Vol 27 (4) ◽  
pp. 1443-1448 ◽  
Author(s):  
Morteza Hosseini ◽  
Shiva Mohammadi ◽  
Yasaman-Sadat Borghei ◽  
Mohammad Reza Ganjali
Keyword(s):  
Gene Mutation ◽  
P53 Gene ◽  
Silver Nanoclusters ◽  
P53 Gene Mutation ◽  
Single Base ◽  
Single Base Mismatch ◽  
Base Mismatch

scholarly journals Digital-resolution detection of microRNA with single-base selectivity by photonic resonator absorption microscopy

2019 ◽  
Vol 116 (39) ◽  
pp. 19362-19367 ◽  
Author(s):  
Taylor D. Canady ◽  
Nantao Li ◽  
Lucas D. Smith ◽  
Yi Lu ◽  
Manish Kohli ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Signal To Noise Ratio ◽  
Resonant Wavelength ◽  
High Signal ◽  
Single Base ◽  
Reflected Light ◽  
Single Base Mismatch ◽  
Base Mismatch ◽  
Digital Resolution ◽  
Target Probe

Circulating exosomal microRNA (miR) represents a new class of blood-based biomarkers for cancer liquid biopsy. The detection of miR at a very low concentration and with single-base discrimination without the need for sophisticated equipment, large volumes, or elaborate sample processing is a challenge. To address this, we present an approach that is highly specific for a target miR sequence and has the ability to provide “digital” resolution of individual target molecules with high signal-to-noise ratio. Gold nanoparticle tags are prepared with thermodynamically optimized nucleic acid toehold probes that, when binding to a target miR sequence, displace a probe-protecting oligonucleotide and reveal a capture sequence that is used to selectively pull down the target-probe–nanoparticle complex to a photonic crystal (PC) biosensor surface. By matching the surface plasmon-resonant wavelength of the nanoparticle tag to the resonant wavelength of the PC nanostructure, the reflected light intensity from the PC is dramatically and locally quenched by the presence of each individual nanoparticle, enabling a form of biosensor microscopy that we call Photonic Resonator Absorption Microscopy (PRAM). Dynamic PRAM imaging of nanoparticle tag capture enables direct 100-aM limit of detection and single-base mismatch selectivity in a 2-h kinetic discrimination assay. The PRAM assay demonstrates that ultrasensitivity (<1 pM) and high selectivity can be achieved on a direct readout diagnostic.

Single base mismatch detection by microsecond voltage pulses

2005 ◽  
Vol 21 (6) ◽  
pp. 888-893 ◽  
Author(s):  
F. Fixe ◽  
V. Chu ◽  
D.M.F. Prazeres ◽  
J.P. Conde
Keyword(s):  
Single Base ◽  
Mismatch Detection ◽  
Single Base Mismatch ◽  
Base Mismatch ◽  
Voltage Pulses
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