Surface-enhanced Raman spectroscopy for simultaneous sensitive detection of multiple microRNAs in lung cancer cells

2014 ◽  
Vol 50 (80) ◽  
pp. 11883-11886 ◽  
Author(s):  
Li-Ping Ye ◽  
Juan Hu ◽  
Li Liang ◽  
Chun-yang Zhang
Keyword(s):  
Lung Cancer ◽  
Raman Spectroscopy ◽  
Cancer Cells ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sensitive Detection ◽  
Lung Cancer Cells ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Label-Free Detection of Human Serum Using Surface-Enhanced Raman Spectroscopy Based on Highly Branched Gold Nanoparticle Substrates for Discrimination of Non-Small Cell Lung Cancer

2018 ◽  
Vol 2018 ◽  
pp. 1-13
Author(s):  
Xiaowei Cao ◽  
Zhenyu Wang ◽  
Liyan Bi ◽  
Jie Zheng
Keyword(s):  
Lung Cancer ◽  
Raman Spectroscopy ◽  
Cell Lung Cancer ◽  
Surface Enhanced Raman Spectroscopy ◽  
Small Cell ◽  
Label Free ◽  
Small Cell Lung ◽  
Surface Enhanced ◽  
Different Types ◽  
Surface Enhanced Raman

Surface-enhanced Raman spectroscopy (SERS) is a good candidate for the development of fast and easy-to-use diagnostic tools, possibly used on serum in screening tests. In this study, a potential label-free serum test based on SERS spectroscopy was developed to analyze human serum for the diagnosis of the non-small cell lung cancer (NSCLC). We firstly synthesized novel highly branched gold nanoparticles (HGNPs) at high yield through a one-step reduction of HAuCl4 with dopamine hydrochloride at 60°C. Then, HGNP substrates with good reproducibility, uniformity, and high SERS effect were fabricated by the electrostatically assisted (3-aminopropyl) triethoxysilane-(APTES-) functionalized silicon wafer surface-sedimentary self-assembly method. Using as-prepared HGNP substrates as a high-performance sensing platform, SERS spectral data of serum obtained from healthy subjects, lung adenocarcinoma patients, lung squamous carcinoma patients, and large cell lung cancer patients were collected. The difference spectra among different types of NSCLC were compared, and analysis result revealed their intrinsic difference in types and contents of nucleic acids, proteins, carbohydrates, amino acids, and lipids. SERS spectra were analyzed by principal component analysis (PCA), which was able to distinguish different types of NSCLC. Considering its time efficiency, being label-free, and sensitivity, SERS based on HGNP substrates is very promising for mass screening NSCLC and plays an important role in the detection and prevention of other diseases.

scholarly journals Designing dendronic-Raman markers for sensitive detection using surface-enhanced Raman spectroscopy

RSC Advances ◽  
2019 ◽  
Vol 9 (48) ◽  
pp. 28222-28227
Author(s):  
Priyanka Jain ◽  
Robi Sankar Patra ◽  
Sridhar Rajaram ◽  
Chandrabhas Narayana
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sensitive Detection ◽  
Trace Detection ◽  
New Approach ◽  
Sers Enhancement ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

A new approach of tuning SERS enhancement with the aid of coupling chemistry for trace detection. A greater number of Raman-active molecules are constrained in a dendronic framework as an improved SERS analyte.

Detection of leucine aminopeptidase activity in serum using surface-enhanced Raman spectroscopy

The Analyst ◽  
2019 ◽  
Vol 144 (4) ◽  
pp. 1394-1400 ◽  
Author(s):  
Dan Guo ◽  
Zhen-Fei Gan ◽  
Lei Jiang ◽  
Mao-Feng Cao ◽  
Fato Tano Patrice ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Leucine Aminopeptidase ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sensitive Detection ◽  
Aminopeptidase Activity ◽  
Specific Reaction ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

A specific reaction-based SERS approach was developed for the selective and sensitive detection of leucine aminopeptidase activity in serum.

Sensitive Detection of Melamine Using Surface Enhanced Raman Spectroscopy on Solid Substrates

2010 ◽  
Author(s):  
Evelyn Kämmer ◽  
Anne März ◽  
Paulo Augusto Da Costa Filho ◽  
Fabien Robert ◽  
Petra Rösch ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Sensitive Detection ◽  
Surface Enhanced ◽  
Solid Substrates ◽  
Surface Enhanced Raman

Uptake of silver nanoparticles by DHA-treated cancer cells examined by surface-enhanced Raman spectroscopy in a microfluidic chip

Lab on a Chip ◽  
2017 ◽  
Vol 17 (7) ◽  
pp. 1306-1313 ◽  
Author(s):  
Zhimin Zhai ◽  
Fengqiu Zhang ◽  
Xiangyu Chen ◽  
Jie Zhong ◽  
Gang Liu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Cancer Cells ◽  
Microfluidic Chip ◽  
Surface Enhanced Raman Spectroscopy ◽  
Living Cells ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

This paper reports on the synthesis and application of biocompatible and sensitive SERS nanoparticles for the study of uptake of nanoparticles into living cells in a microfluidic chip through surface-enhanced Raman spectroscopy (SERS).

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