Folding-based photoelectrochemical biosensor: binding-induced conformation change of a quantum dot-tagged DNA probe for mercury(ii) detection

2014 ◽  
Vol 50 (81) ◽  
pp. 12088-12090 ◽  
Author(s):  
Zheng-Yuan Ma ◽  
Jian-Bin Pan ◽  
Cai-Yu Lu ◽  
Wei-Wei Zhao ◽  
Jing-Juan Xu ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Dna Probe ◽  
Conformation Change ◽  
Photoelectrochemical Biosensor

scholarly journals Detection of Hepatitis B Virus M204I Mutation by Quantum Dot-Labeled DNA Probe

Sensors ◽  
2017 ◽  
Vol 17 (5) ◽  
pp. 961 ◽  
Author(s):  
Cheng Zhang ◽  
Yiping Chen ◽  
Xinmiao Liang ◽  
Guanhua Zhang ◽  
Hong Ma ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Quantum Dot ◽  
Dna Probe ◽  
B Virus

Amplified photoelectrochemical DNA biosensor based on a CdS quantum dot/WS2 nanosheet heterojunction and hybridization chain reaction-mediated enzymatic hydrolysis

2019 ◽  
Vol 11 (16) ◽  
pp. 2163-2169 ◽  
Author(s):  
Yun Ju ◽  
Xin Hu ◽  
Yang Zang ◽  
Rong Cao ◽  
Huaiguo Xue
Keyword(s):  
Enzymatic Hydrolysis ◽  
Quantum Dot ◽  
Dna Biosensor ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Cds Quantum Dot ◽  
Photoelectrochemical Biosensor

An amplified photoelectrochemical biosensor was designed for the ultrasensitive monitoring of DNA by coupling a CdS quantum dot (QD)/WS2 nanosheet heterostructure with hybridization chain reaction (HCR)-programmed enzymatic hydrolysis.

Hybrid PbS Quantum Dot/Nanoporous NiO Film Nanostructure: Preparation, Characterization, and Application for a Self-Powered Cathodic Photoelectrochemical Biosensor

2017 ◽  
Vol 89 (15) ◽  
pp. 8070-8078 ◽  
Author(s):  
Wen-Xia Dai ◽  
Ling Zhang ◽  
Wei-Wei Zhao ◽  
Xiao-Dong Yu ◽  
Jing-Juan Xu ◽  
...  
Keyword(s):  
Quantum Dot ◽  
Photoelectrochemical Biosensor ◽  
Self Powered ◽  
Pbs Quantum Dot

Quantum Dot-Bead-DNA Probe-Based Hybridization Fluorescence Assays on Microfluidic Chips

2015 ◽  
Vol 15 (10) ◽  
pp. 7918-7921 ◽  
Author(s):  
Seshadri Reddy Ankireddy ◽  
Jongsung Kim
Keyword(s):  
Quantum Dot ◽  
Simultaneous Detection ◽  
Dna Probe ◽  
Detection Methods ◽  
Microfluidic Chips ◽  
Wild Type ◽  
Research Focus ◽  
Target Dna ◽  
Type Sequence ◽  
Hybridization Detection

The development of chip-based, quantum dot (QD)-bead-DNA conjugate probes for hybridization detection is a prime research focus in the field of microfluidics. QD-Bead-DNA probe-based hybridization detection methods are often called “bead-based assays,” and their success is substantially influenced by the dispensing and manipulation capabilities of microfluidic technology. Met was identified as a prognostic marker in different cancers including lung, renal, liver, head and neck, stomach, and breast. In this report, the cancer causing Met gene was detected with QDs attached to polystyrene microbeads. We constructed a microfluidic platform using a flexible PDMS polymer. The chip consists of two channels, with two inlets and two outlets. The two channels were integrated with QD-bead-DNA probes for simultaneous detection of wild type target DNA and mutant DNA, containing three nucleotide changes compared to the wild type sequence. The fluorescence quenching ability of QDs within the channels of microfluidic chips were compared for both DNAs.

An electrochemiluminescence sensor based on CdSe@CdS-functionalized MoS2 and a GOD-labeled DNA probe for the sensitive detection of Hg(ii)

2020 ◽  
Vol 12 (4) ◽  
pp. 491-498 ◽  
Author(s):  
Zhang-Jin He ◽  
Tian-Fang Kang ◽  
Li-Ping Lu ◽  
Shui-Yuan Cheng
Keyword(s):  
Quantum Dot ◽  
Modified Electrode ◽  
Dna Probe ◽  
Sensitive Detection ◽  
Mercury Ions ◽  
Cds Quantum Dot

In this study, a novel electrochemiluminescence (ECL) biosensor based on a CdSe@CdS quantum dot (QD)-functionalized MoS2-modified electrode was developed for the sensitive detection of mercury ions.

scholarly journals Development of CdSe–ZnO Flower-Rod Core-Shell Structure Based Photoelectrochemical Biosensor for Detection of Norovirous RNA

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 2980 ◽  
Author(s):  
Zhizhong Han ◽  
Qinghua Weng ◽  
Chaofan Lin ◽  
Jinquan Yi ◽  
Jie Kang
Keyword(s):  
Visible Light ◽  
Shell Structure ◽  
Measurement Range ◽  
Dna Probe ◽  
Core Shell ◽  
Absorbance Spectrum ◽  
Exchange Method ◽  
Photoelectrochemical Biosensor ◽  
Ion Exchange Method ◽  
Core Shell Structure

In this work, the CdSe–ZnO flower-rod core-shell structure (CSZFRs) was prepared by ion-exchange method. The surface of CSZFRs was modified by 3-mercaptopropionic acid (MPA), and then the DNA probe was immobilized on the surface via chemical bond between -NH2 of DNA probe and -COOH of MPA. Finally, the target norovirous (NV) RNA was combined with the probe according to the principle of complementary base pairing, resulting in a decrease of the photocurrent. The results show that the absorbance spectrum of visible light is enhanced for CSZFRs compared with pure ZnO. Under visible light irradiation, the photocurrent of CSZFRs is up to 0.1 mA, which can improve the sensitivity of the photoelectrochemical (PEC) biosensor. In the measurement range of 0–5.10 nM, the measured concentrations (c) have a good linear relationship with the output photocurrent of the biosensor. The linear regression equation is expressed as I = 0.03256 − 0.0033c (R2 = 0.99, S/N = 3) with a detection limit of 0.50 nM. Therefore, this work realizes a rapid and sensitive method for the detection of NV RNA.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

Biodistribution 18F-markierter Quantum Dot-Antikörper-Biokonjugate

2019 ◽  
Author(s):  
O Mishchenko ◽  
A Schildan ◽  
O Sabri ◽  
M Patt
Keyword(s):  
Quantum Dot
Sign in / Sign up

Export Citation Format

Share Document